Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model
Due to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the ant...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/8626987 |
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| author | Livia Aparecida Procópio Gomes Lívia Mara Alves Figueiredo Ana Luiza do Rosário Palma Barbara Maria Corrêa Geraldo Kelly Cristine Isler Castro Luciana Ruano de Oliveira Fugisaki Antônio Olavo Cardoso Jorge Luciane Dias de Oliveira Juliana Campos Junqueira |
| author_facet | Livia Aparecida Procópio Gomes Lívia Mara Alves Figueiredo Ana Luiza do Rosário Palma Barbara Maria Corrêa Geraldo Kelly Cristine Isler Castro Luciana Ruano de Oliveira Fugisaki Antônio Olavo Cardoso Jorge Luciane Dias de Oliveira Juliana Campos Junqueira |
| author_sort | Livia Aparecida Procópio Gomes |
| collection | DOAJ |
| description | Due to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P<0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella. |
| format | Article |
| id | doaj-art-af248a3d9f7b473f9991cbf73beb6399 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-af248a3d9f7b473f9991cbf73beb63992025-02-03T01:01:51ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/86269878626987Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella ModelLivia Aparecida Procópio Gomes0Lívia Mara Alves Figueiredo1Ana Luiza do Rosário Palma2Barbara Maria Corrêa Geraldo3Kelly Cristine Isler Castro4Luciana Ruano de Oliveira Fugisaki5Antônio Olavo Cardoso Jorge6Luciane Dias de Oliveira7Juliana Campos Junqueira8Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDepartment of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, BrazilDue to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P<0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella.http://dx.doi.org/10.1155/2016/8626987 |
| spellingShingle | Livia Aparecida Procópio Gomes Lívia Mara Alves Figueiredo Ana Luiza do Rosário Palma Barbara Maria Corrêa Geraldo Kelly Cristine Isler Castro Luciana Ruano de Oliveira Fugisaki Antônio Olavo Cardoso Jorge Luciane Dias de Oliveira Juliana Campos Junqueira Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model The Scientific World Journal |
| title | Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model |
| title_full | Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model |
| title_fullStr | Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model |
| title_full_unstemmed | Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model |
| title_short | Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model |
| title_sort | punica granatum l pomegranate extract in vivo study of antimicrobial activity against porphyromonas gingivalis in galleria mellonella model |
| url | http://dx.doi.org/10.1155/2016/8626987 |
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