Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides
The study aimed to establish the effective extraction methods for Chlamydomonas reinhardtii proteins and screen peptide segments with inhibitory effects on monoamine oxidase A (MAO-A). The proteins were extracted, separated, and purified by swelling, ultrasonication, alkaline dissolution, and saltin...
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The editorial department of Science and Technology of Food Industry
2025-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070176 |
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| author | Meiting LIU Ya MAO Jintao XIE Wenxia LIU Lixia WU Yishan WU Mei WANG Xuewu ZHANG |
| author_facet | Meiting LIU Ya MAO Jintao XIE Wenxia LIU Lixia WU Yishan WU Mei WANG Xuewu ZHANG |
| author_sort | Meiting LIU |
| collection | DOAJ |
| description | The study aimed to establish the effective extraction methods for Chlamydomonas reinhardtii proteins and screen peptide segments with inhibitory effects on monoamine oxidase A (MAO-A). The proteins were extracted, separated, and purified by swelling, ultrasonication, alkaline dissolution, and salting-out. Subsequently, the polypeptide content, amino acid composition, molecular weight distribution and the MAO-A inhibitory effect of proteins treated with enzymatic hydrolysis and ultrafiltration were determined. Peptide sequences with high inhibitory potential were selected by bioinformatics tools and molecular docking with MAO-A was applied to analyze the binding sites. The results indicated that a protein extraction yield of 50.98%±2.50% was achieved through swelling for 4 h, followed by four cycles of 240 W ultrasonication (10 min per cycle), alkaline dissolution at pH9.5, acid precipitation at pH3.5, and the treatment with saturated ammonium sulfate at a ratio of 1:2. Notably, the alkaline protease hydrolysate (APH) and neutral protease hydrolysate (NPH) exhibited high inhibitory effects on MAO-A (P<0.05), with IC50 values of 3.437 and 2.699 mg/mL, respectively. The fractions with molecular weights less than 3 kDa from APH and NPH (APH-Ⅲ and NPH-Ⅲ) displayed significantly higher MAO-A inhibitory effect (P<0.05), with IC50 values of 3.116 and 0.909 mg/mL, respectively. Molecular docking results showed that two peptides, ASDDAVHGWGGPGGY and AYSGETQSEGGFAPNR from NPH-Ⅲ, could effectively bind to the active sites His488, Glu286, Tyr53, Tyr268, Asp343 and Glu485 of MAO-A. |
| format | Article |
| id | doaj-art-af1cf223ef104956bd14e28acd3b09b1 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-af1cf223ef104956bd14e28acd3b09b12025-08-20T02:08:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-01461222623610.13386/j.issn1002-0306.20240701762024070176-12Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory PeptidesMeiting LIU0Ya MAO1Jintao XIE2Wenxia LIU3Lixia WU4Yishan WU5Mei WANG6Xuewu ZHANG7College of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaZhanjiang Institute of Supervision and Test on Quality & Measurement, Zhanjiang 524094, ChinaCollege of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaThe study aimed to establish the effective extraction methods for Chlamydomonas reinhardtii proteins and screen peptide segments with inhibitory effects on monoamine oxidase A (MAO-A). The proteins were extracted, separated, and purified by swelling, ultrasonication, alkaline dissolution, and salting-out. Subsequently, the polypeptide content, amino acid composition, molecular weight distribution and the MAO-A inhibitory effect of proteins treated with enzymatic hydrolysis and ultrafiltration were determined. Peptide sequences with high inhibitory potential were selected by bioinformatics tools and molecular docking with MAO-A was applied to analyze the binding sites. The results indicated that a protein extraction yield of 50.98%±2.50% was achieved through swelling for 4 h, followed by four cycles of 240 W ultrasonication (10 min per cycle), alkaline dissolution at pH9.5, acid precipitation at pH3.5, and the treatment with saturated ammonium sulfate at a ratio of 1:2. Notably, the alkaline protease hydrolysate (APH) and neutral protease hydrolysate (NPH) exhibited high inhibitory effects on MAO-A (P<0.05), with IC50 values of 3.437 and 2.699 mg/mL, respectively. The fractions with molecular weights less than 3 kDa from APH and NPH (APH-Ⅲ and NPH-Ⅲ) displayed significantly higher MAO-A inhibitory effect (P<0.05), with IC50 values of 3.116 and 0.909 mg/mL, respectively. Molecular docking results showed that two peptides, ASDDAVHGWGGPGGY and AYSGETQSEGGFAPNR from NPH-Ⅲ, could effectively bind to the active sites His488, Glu286, Tyr53, Tyr268, Asp343 and Glu485 of MAO-A.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070176chlamydomonas reinhardtiiprotein extractionmao-a inhibitory peptidesmolecular docking |
| spellingShingle | Meiting LIU Ya MAO Jintao XIE Wenxia LIU Lixia WU Yishan WU Mei WANG Xuewu ZHANG Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides Shipin gongye ke-ji chlamydomonas reinhardtii protein extraction mao-a inhibitory peptides molecular docking |
| title | Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides |
| title_full | Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides |
| title_fullStr | Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides |
| title_full_unstemmed | Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides |
| title_short | Extraction of Proteins from Chlamydomonas reinhardtii and Screening of MAO-A Inhibitory Peptides |
| title_sort | extraction of proteins from chlamydomonas reinhardtii and screening of mao a inhibitory peptides |
| topic | chlamydomonas reinhardtii protein extraction mao-a inhibitory peptides molecular docking |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070176 |
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