Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved]
Background In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal hist...
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2025-07-01
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| author | Hery Purnobasuki Erma Safitri Sri Hidanah Mitsuhiro Takagi Goo Jang Siti Darodjah Rasad Martia Rani Tacharina Jola Rahmahani Sri Mulyati Chairul Anwar Nidom Mohammad Anam Al Arif Muhammad Thohawi Elziyad Purnama Eka Pramyrtha Hestianah Maulida Ilma Sadida Merisa Wahyu Erdhina Suzanita Utama Tita Damayanti Lestari |
| author_facet | Hery Purnobasuki Erma Safitri Sri Hidanah Mitsuhiro Takagi Goo Jang Siti Darodjah Rasad Martia Rani Tacharina Jola Rahmahani Sri Mulyati Chairul Anwar Nidom Mohammad Anam Al Arif Muhammad Thohawi Elziyad Purnama Eka Pramyrtha Hestianah Maulida Ilma Sadida Merisa Wahyu Erdhina Suzanita Utama Tita Damayanti Lestari |
| author_sort | Hery Purnobasuki |
| collection | DOAJ |
| description | Background In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal histopathology ameliorations against ND in vaccinated broilers with aflatoxin B1 (AFB1) and ochratoxin A (OTA) toxication. Methods A total of 20 broilers were randomly assigned into 4 groups with 5 replications i.e. (C-) broiler groups with basal feed, (C+) broiler groups with AFB1 and OTA feed contamination, (T1) and (T2) broiler groups with exposed AFB1, OTA, and toxin binders as feed additives with dose 1.1 g/kg and 1.6 g/kg feed, respectively. ND vaccination was carried out on day 7 and 21. Antibody titers were evaluated from serum samples of broiler on days 14, 28, and 35 for further hemagglutination inhibition (HI) test. Histopathology of the cecum and colon organs was evaluated using HE staining on day 36. HI test and histological scoring were analyzed using the One-Way ANOVA, followed by Duncan’s test with a p < 0.05 in SPSS v.26 software (IBM Corp., Armonk, NY). Results As a result, histopathological improvement of the cecum and colon was reported based on mucosal rupture, hemorrhage and necrosis on day 35. A significant increase in antibody titer was observed on day 35 in the C− and T2 groups, which differed significantly (p < 0.05) from the C+ and T1 groups. Conclusions This study revealed that a 1.6 g/kg toxin binder dose in feed can increase antibody titer and histopathology of cecum and colon in broiler chickens after ND vaccination fed with mycotoxin-contaminated feed. |
| format | Article |
| id | doaj-art-af18d7c58a434df09af89c1261995e00 |
| institution | DOAJ |
| issn | 2046-1402 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-af18d7c58a434df09af89c1261995e002025-08-20T03:07:13ZengF1000 Research LtdF1000Research2046-14022025-07-011310.12688/f1000research.158103.3185498Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved]Hery Purnobasuki0Erma Safitri1Sri Hidanah2Mitsuhiro Takagi3Goo Jang4Siti Darodjah Rasad5Martia Rani Tacharina6Jola Rahmahani7Sri Mulyati8Chairul Anwar Nidom9Mohammad Anam Al Arif10Muhammad Thohawi Elziyad Purnama11https://orcid.org/0000-0002-9496-0330Eka Pramyrtha Hestianah12Maulida Ilma Sadida13Merisa Wahyu Erdhina14Suzanita Utama15Tita Damayanti Lestari16Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaLaboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Yamaguchi Prefecture, 753-8515, JapanDepartment of Theriogenology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul, 151-742, South KoreaDepartment of Animal Production, Faculty of Animal Science, Universitas Padjadjaran, Sumedang, West Java, 45363, IndonesiaDepartment of Veterinary Science, Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaUndergraduate Student, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaUndergraduate Student, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaDepartment of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, IndonesiaBackground In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal histopathology ameliorations against ND in vaccinated broilers with aflatoxin B1 (AFB1) and ochratoxin A (OTA) toxication. Methods A total of 20 broilers were randomly assigned into 4 groups with 5 replications i.e. (C-) broiler groups with basal feed, (C+) broiler groups with AFB1 and OTA feed contamination, (T1) and (T2) broiler groups with exposed AFB1, OTA, and toxin binders as feed additives with dose 1.1 g/kg and 1.6 g/kg feed, respectively. ND vaccination was carried out on day 7 and 21. Antibody titers were evaluated from serum samples of broiler on days 14, 28, and 35 for further hemagglutination inhibition (HI) test. Histopathology of the cecum and colon organs was evaluated using HE staining on day 36. HI test and histological scoring were analyzed using the One-Way ANOVA, followed by Duncan’s test with a p < 0.05 in SPSS v.26 software (IBM Corp., Armonk, NY). Results As a result, histopathological improvement of the cecum and colon was reported based on mucosal rupture, hemorrhage and necrosis on day 35. A significant increase in antibody titer was observed on day 35 in the C− and T2 groups, which differed significantly (p < 0.05) from the C+ and T1 groups. Conclusions This study revealed that a 1.6 g/kg toxin binder dose in feed can increase antibody titer and histopathology of cecum and colon in broiler chickens after ND vaccination fed with mycotoxin-contaminated feed.https://f1000research.com/articles/13-1518/v3aflatoxin ochratoxin antibody titer broiler toxin binders food productioneng |
| spellingShingle | Hery Purnobasuki Erma Safitri Sri Hidanah Mitsuhiro Takagi Goo Jang Siti Darodjah Rasad Martia Rani Tacharina Jola Rahmahani Sri Mulyati Chairul Anwar Nidom Mohammad Anam Al Arif Muhammad Thohawi Elziyad Purnama Eka Pramyrtha Hestianah Maulida Ilma Sadida Merisa Wahyu Erdhina Suzanita Utama Tita Damayanti Lestari Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] F1000Research aflatoxin ochratoxin antibody titer broiler toxin binders food production eng |
| title | Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] |
| title_full | Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] |
| title_fullStr | Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] |
| title_full_unstemmed | Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] |
| title_short | Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers [version 3; peer review: 1 approved, 3 approved with reservations, 1 not approved] |
| title_sort | mycotoxin binder for immune and intestinal histopathology ameliorations against newcastle disease in vaccinated broilers version 3 peer review 1 approved 3 approved with reservations 1 not approved |
| topic | aflatoxin ochratoxin antibody titer broiler toxin binders food production eng |
| url | https://f1000research.com/articles/13-1518/v3 |
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