Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability

Chitin is the second most prevalent polysaccharide found in nature, following cellulose. Amino-oligosaccharides, the byproducts of chitin degradation, exhibit favorable biological properties and potential for various uses. Chitinases play a crucial function in the breakdown of chitin, and their exce...

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Main Authors: Mati Ullah, Yutong Xia, Dalal Sulaiman Alshaya, Jianda Han, Kotb A. Attia, Tawaf Ali Shah, Huayou Chen
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/18/4302
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author Mati Ullah
Yutong Xia
Dalal Sulaiman Alshaya
Jianda Han
Kotb A. Attia
Tawaf Ali Shah
Huayou Chen
author_facet Mati Ullah
Yutong Xia
Dalal Sulaiman Alshaya
Jianda Han
Kotb A. Attia
Tawaf Ali Shah
Huayou Chen
author_sort Mati Ullah
collection DOAJ
description Chitin is the second most prevalent polysaccharide found in nature, following cellulose. Amino-oligosaccharides, the byproducts of chitin degradation, exhibit favorable biological properties and potential for various uses. Chitinases play a crucial function in the breakdown of chitin, and their exceptionally effective production has garnered significant interest. Here, in this study, the exochitinase PbChiA, obtained from <i>Paenibacillus barengoltzii</i>, was recombinantly produced and immobilized using the CotG surface protein of <i>Bacillus subtilis</i> WB800N. The resulting strain <i>Bacillus subtilis</i> WB800N pHS-CotG-Chi exhibited exceptional heat stability and efficacy across various pH levels. The chitinolytic activity of the enzyme, which had been isolated and immobilized on the spore surface, was measured to be approximately 16.06 U/mL. Including Ni<sup>2+</sup>, Zn<sup>+2</sup>, and K<sup>+</sup>, and EDTA at various concentration levels in the reaction system, has significantly enhanced the activity of the immobilized enzyme. The immobilized exochitinase demonstrated a notable rate of recycling, as the recombinant spores sustained a relative enzyme activity of more than 70% after three cycles and 62.7% after four cycles. These findings established a basis for additional investigation into the role and practical use of the immobilized bacterial exochitinase in industry.
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spelling doaj-art-af16fe9aca1442f1b7dacba5f3f84b072025-08-20T01:55:42ZengMDPI AGMolecules1420-30492024-09-012918430210.3390/molecules29184302Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and RecyclabilityMati Ullah0Yutong Xia1Dalal Sulaiman Alshaya2Jianda Han3Kotb A. Attia4Tawaf Ali Shah5Huayou Chen6School of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaSchool of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaCenter of Excellence in Biotechnology Research, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaCollege of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo 255000, ChinaSchool of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaChitin is the second most prevalent polysaccharide found in nature, following cellulose. Amino-oligosaccharides, the byproducts of chitin degradation, exhibit favorable biological properties and potential for various uses. Chitinases play a crucial function in the breakdown of chitin, and their exceptionally effective production has garnered significant interest. Here, in this study, the exochitinase PbChiA, obtained from <i>Paenibacillus barengoltzii</i>, was recombinantly produced and immobilized using the CotG surface protein of <i>Bacillus subtilis</i> WB800N. The resulting strain <i>Bacillus subtilis</i> WB800N pHS-CotG-Chi exhibited exceptional heat stability and efficacy across various pH levels. The chitinolytic activity of the enzyme, which had been isolated and immobilized on the spore surface, was measured to be approximately 16.06 U/mL. Including Ni<sup>2+</sup>, Zn<sup>+2</sup>, and K<sup>+</sup>, and EDTA at various concentration levels in the reaction system, has significantly enhanced the activity of the immobilized enzyme. The immobilized exochitinase demonstrated a notable rate of recycling, as the recombinant spores sustained a relative enzyme activity of more than 70% after three cycles and 62.7% after four cycles. These findings established a basis for additional investigation into the role and practical use of the immobilized bacterial exochitinase in industry.https://www.mdpi.com/1420-3049/29/18/4302bacterial exochitinase<i>Bacillus substilis</i> WB800Nspore surface display
spellingShingle Mati Ullah
Yutong Xia
Dalal Sulaiman Alshaya
Jianda Han
Kotb A. Attia
Tawaf Ali Shah
Huayou Chen
Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
Molecules
bacterial exochitinase
<i>Bacillus substilis</i> WB800N
spore surface display
title Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
title_full Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
title_fullStr Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
title_full_unstemmed Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
title_short Display of Bacterial Exochitanase on <i>Bacillus subtilis</i> Spores Improved Enzyme Stability and Recyclability
title_sort display of bacterial exochitanase on i bacillus subtilis i spores improved enzyme stability and recyclability
topic bacterial exochitinase
<i>Bacillus substilis</i> WB800N
spore surface display
url https://www.mdpi.com/1420-3049/29/18/4302
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