Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity
Anthocyanin is an important flavonoid with antioxidant, anticancer, and anti-inflammatory properties. This research investigates the anthocyanin content of Cavendish banana inflorescence, a by-product often discarded as agricultural waste. The study employs two drying methods, namely oven-drying and...
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2024-11-01
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| author | Nuwanthi Senevirathna Morteza Hassanpour Ian O’Hara Azharul Karim |
| author_facet | Nuwanthi Senevirathna Morteza Hassanpour Ian O’Hara Azharul Karim |
| author_sort | Nuwanthi Senevirathna |
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| description | Anthocyanin is an important flavonoid with antioxidant, anticancer, and anti-inflammatory properties. This research investigates the anthocyanin content of Cavendish banana inflorescence, a by-product often discarded as agricultural waste. The study employs two drying methods, namely oven-drying and freeze-drying, followed by accelerated solvent extraction using acidified water and methanol. Liquid chromatography-mass spectroscopy (LC-MS) results confirm banana inflorescence as a rich source of anthocyanins. According to LC-MS analysis, freeze-dried banana inflorescence extracted in methanol at 80 °C exhibits the highest anthocyanin content (130.01 mg/100 g). This sample also demonstrates superior characteristics, including a chroma value of 40.02 ± 0.01, a redness value of 38.09 ± 0.16, 18.46 ± 0.02 °Brix, a total phenolic content of 42.5 ± 1.00 mg/g, expressed as gallic acid equivalents, and a total antioxidant activity of 71.33 ± 0.08% when assessed with the DPPH method. Furthermore, the study identifies the predominant anthocyanin as cyanidin, along with the presence of other anthocyanins such as delphinidin (Dp), malvidin (Mv), petunidin (Pt), pelargonidin (Pg), and peonidin (Pn). Interestingly, the extracted anthocyanins demonstrate pH sensitivity, changing from red to brown as pH increases. These findings highlight the potential of utilizing Cavendish banana inflorescence for anthocyanin extraction, offering sustainable waste valorization methods with promising applications in biomimetics and bioinspiration fields. |
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| spelling | doaj-art-af10ab467d6445c8a6a16a5beb4a1c2b2025-08-20T02:08:08ZengMDPI AGBiomimetics2313-76732024-11-0191170210.3390/biomimetics9110702Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH SensitivityNuwanthi Senevirathna0Morteza Hassanpour1Ian O’Hara2Azharul Karim3School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, AustraliaSchool of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, AustraliaSchool of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, AustraliaSchool of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, AustraliaAnthocyanin is an important flavonoid with antioxidant, anticancer, and anti-inflammatory properties. This research investigates the anthocyanin content of Cavendish banana inflorescence, a by-product often discarded as agricultural waste. The study employs two drying methods, namely oven-drying and freeze-drying, followed by accelerated solvent extraction using acidified water and methanol. Liquid chromatography-mass spectroscopy (LC-MS) results confirm banana inflorescence as a rich source of anthocyanins. According to LC-MS analysis, freeze-dried banana inflorescence extracted in methanol at 80 °C exhibits the highest anthocyanin content (130.01 mg/100 g). This sample also demonstrates superior characteristics, including a chroma value of 40.02 ± 0.01, a redness value of 38.09 ± 0.16, 18.46 ± 0.02 °Brix, a total phenolic content of 42.5 ± 1.00 mg/g, expressed as gallic acid equivalents, and a total antioxidant activity of 71.33 ± 0.08% when assessed with the DPPH method. Furthermore, the study identifies the predominant anthocyanin as cyanidin, along with the presence of other anthocyanins such as delphinidin (Dp), malvidin (Mv), petunidin (Pt), pelargonidin (Pg), and peonidin (Pn). Interestingly, the extracted anthocyanins demonstrate pH sensitivity, changing from red to brown as pH increases. These findings highlight the potential of utilizing Cavendish banana inflorescence for anthocyanin extraction, offering sustainable waste valorization methods with promising applications in biomimetics and bioinspiration fields.https://www.mdpi.com/2313-7673/9/11/702anthocyaninantioxidantbanana inflorescencecyanidinpH-sensitive |
| spellingShingle | Nuwanthi Senevirathna Morteza Hassanpour Ian O’Hara Azharul Karim Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity Biomimetics anthocyanin antioxidant banana inflorescence cyanidin pH-sensitive |
| title | Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity |
| title_full | Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity |
| title_fullStr | Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity |
| title_full_unstemmed | Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity |
| title_short | Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity |
| title_sort | extraction isolation identification and characterization of anthocyanin from banana inflorescence by liquid chromatography mass spectroscopy and its ph sensitivity |
| topic | anthocyanin antioxidant banana inflorescence cyanidin pH-sensitive |
| url | https://www.mdpi.com/2313-7673/9/11/702 |
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