Multidrug Resistance, Biofilm-Forming Ability, and Molecular Characterization of <i>Vibrio</i> Species Isolated from Foods in Thailand
Background: <i>Vibrio</i> species are common foodborne pathogens that cause gastrointestinal tract inflammation. Multidrug resistance (MDR) in <i>Vibrio</i> spp. is a global health concern, especially in aquaculture systems and food chain systems. This study aimed to detect &...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/14/3/235 |
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| Summary: | Background: <i>Vibrio</i> species are common foodborne pathogens that cause gastrointestinal tract inflammation. Multidrug resistance (MDR) in <i>Vibrio</i> spp. is a global health concern, especially in aquaculture systems and food chain systems. This study aimed to detect <i>Vibrio</i> contamination in food collected from 14 markets in Nakhon Si Thammarat, Thailand, and determine their antibiotic susceptibility. Methods: One hundred and thirty-six food samples were investigated for <i>Vibrio</i> contamination. All isolates were tested for antibiogram and biofilm-forming ability. Moreover, the ceftazidime or cefotaxime resistance isolates were additionally investigated for extended-spectrum β-lactamase (ESBL) producers. The isolates were additionally examined for the presence of antibiotic resistance genes. The ESBL-suspected isolates with moderate-to-high biofilm-forming ability were further analyzed for their whole genome. Results: The prevalence of <i>Vibrio</i> contamination in food samples was 42.65%, with <i>V. parahaemolyticus</i> demonstrating the highest prevalence. Most isolates were resistant to β-lactam antibiotics, followed by aminoglycosides. The overall MDR of isolated <i>Vibrio</i> was 18.29%, with an average multiple antibiotic resistance (MAR) index of 16.41%. Most isolates were found to have β-lactam resistance-related genes (<i>bla</i><sub>TEM</sub>) for 41.46%, followed by aminoglycoside resistance genes (<i>aac</i>(<i>6′</i>)-<i>Ib</i>) for 18.29%. Most <i>Vibrio</i> showed moderate to strong biofilm-forming ability, particularly in MDR isolates (92.86%). Two ESBL-suspected isolates, one <i>V. parahaemolyticus</i> isolate and one <i>V. navarrensis</i>, were sequenced. Interestingly, <i>V. parahaemolyticus</i> was an ESBL producer that harbored the <i>bla</i><sub>CTX-M-55</sub> gene located in the mobile genetic element region. While <i>V. navarrensis</i> was not ESBL producer, this isolate carried the <i>bla</i><sub>AmpC</sub> gene in the region of horizontal gene transfer event. Remarkably, the <i>Inoviridae</i> sp. DNA integration event was present in two <i>Vibrio</i> genomes. Conclusions: These findings impact the understanding of antibiotic-resistant <i>Vibrio</i> spp. in food samples, which could be applied for implementing control measures in aquaculture farming and food safety plans. |
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| ISSN: | 2079-6382 |