Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways
Abstract Background Cholangiocarcinoma (CCA) represents a significant global health concern. The gut and bile microbiota, which can influence the gut-liver axis and disease progression, have not been thoroughly characterized in CCA patients. Methods We selected two clinical centers at our hospital a...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12876-025-04096-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849332943108964352 |
|---|---|
| author | Tianyu De Tian Ma Wei Wang Xusheng An Di Liu Hongkun Yin Qi Wang Ting Zhao Hao Wang |
| author_facet | Tianyu De Tian Ma Wei Wang Xusheng An Di Liu Hongkun Yin Qi Wang Ting Zhao Hao Wang |
| author_sort | Tianyu De |
| collection | DOAJ |
| description | Abstract Background Cholangiocarcinoma (CCA) represents a significant global health concern. The gut and bile microbiota, which can influence the gut-liver axis and disease progression, have not been thoroughly characterized in CCA patients. Methods We selected two clinical centers at our hospital and collected stool samples from CCA patients and healthy controls (HC). These samples underwent whole-genome metagenomic shotgun sequencing, followed by analysis using both marker gene-based and assembly-based methods. Additionally, KEGG pathway enrichment was performed using the cholangiocarcinoma (CHOL) RNA-seq samples. Results Our results revealed distinct dysbiosis of the gut microbiota in our regional CCA patients. The results revealed greater heterogeneity in the gut microbiome of CCA patients compared to HC samples. We found Blautia species to be significantly less abundant in CCA samples, and can distinguish CCA patients from HC. Blautia can also play a role in influencing the modification of secondary bile acids. Additionally, down-regulation of arachidonic acid and linoleic acid metabolism was observed in the tumor tissues of CHOL patients. In summary, the results revealed significant heterogeneity difference in the gut microbiome of CCA patients compared to HC samples, and detected the specifically decreased Blautia species in CCA patients, suggesting that Blautia may influence bile acid metabolic pathways. Further investigation is warranted to explore Blautia as a potential biomarker for CCA. |
| format | Article |
| id | doaj-art-aefa75a3ddfc4a2a9c5a4741868aff0d |
| institution | Kabale University |
| issn | 1471-230X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj-art-aefa75a3ddfc4a2a9c5a4741868aff0d2025-08-20T03:46:03ZengBMCBMC Gastroenterology1471-230X2025-07-0125111310.1186/s12876-025-04096-3Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathwaysTianyu De0Tian Ma1Wei Wang2Xusheng An3Di Liu4Hongkun Yin5Qi Wang6Ting Zhao7Hao Wang8General Hospital of Ningxia Medical UniversityGeneral Hospital of Ningxia Medical UniversityGeneral Hospital of Ningxia Medical UniversityPeking University First Hospital Ningxia Women and Children’s HospitalGeneral Hospital of Ningxia Medical UniversitySchool of Clinical Medicine, Ningxia Medical UniversityGeneral Hospital of Ningxia Medical UniversityPeking University First Hospital Ningxia Women and Children’s HospitalDepartment of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical UniversityAbstract Background Cholangiocarcinoma (CCA) represents a significant global health concern. The gut and bile microbiota, which can influence the gut-liver axis and disease progression, have not been thoroughly characterized in CCA patients. Methods We selected two clinical centers at our hospital and collected stool samples from CCA patients and healthy controls (HC). These samples underwent whole-genome metagenomic shotgun sequencing, followed by analysis using both marker gene-based and assembly-based methods. Additionally, KEGG pathway enrichment was performed using the cholangiocarcinoma (CHOL) RNA-seq samples. Results Our results revealed distinct dysbiosis of the gut microbiota in our regional CCA patients. The results revealed greater heterogeneity in the gut microbiome of CCA patients compared to HC samples. We found Blautia species to be significantly less abundant in CCA samples, and can distinguish CCA patients from HC. Blautia can also play a role in influencing the modification of secondary bile acids. Additionally, down-regulation of arachidonic acid and linoleic acid metabolism was observed in the tumor tissues of CHOL patients. In summary, the results revealed significant heterogeneity difference in the gut microbiome of CCA patients compared to HC samples, and detected the specifically decreased Blautia species in CCA patients, suggesting that Blautia may influence bile acid metabolic pathways. Further investigation is warranted to explore Blautia as a potential biomarker for CCA.https://doi.org/10.1186/s12876-025-04096-3CholangiocarcinomaBacterial floraBlautiaMicrobiomarkers |
| spellingShingle | Tianyu De Tian Ma Wei Wang Xusheng An Di Liu Hongkun Yin Qi Wang Ting Zhao Hao Wang Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways BMC Gastroenterology Cholangiocarcinoma Bacterial flora Blautia Microbiomarkers |
| title | Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways |
| title_full | Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways |
| title_fullStr | Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways |
| title_full_unstemmed | Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways |
| title_short | Intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated Blautia species and bile acid metabolic pathways |
| title_sort | intestinal microbiota in adults with cholangiocarcinoma identifies the dysregulated blautia species and bile acid metabolic pathways |
| topic | Cholangiocarcinoma Bacterial flora Blautia Microbiomarkers |
| url | https://doi.org/10.1186/s12876-025-04096-3 |
| work_keys_str_mv | AT tianyude intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT tianma intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT weiwang intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT xushengan intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT diliu intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT hongkunyin intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT qiwang intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT tingzhao intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways AT haowang intestinalmicrobiotainadultswithcholangiocarcinomaidentifiesthedysregulatedblautiaspeciesandbileacidmetabolicpathways |