Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad
Leishmaniasis is a group of parasitic diseases caused by Leishmania spp., an endemic infectious agent in developing countries, including Iraq. Diagnosis of cutaneous lesion by stained smears, serology or histopathology are inaccurate and unable to detect the species of Leishmania. Here, two molecula...
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| Language: | English |
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University of Baghdad, College of Science for Women
2021-03-01
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| Series: | مجلة بغداد للعلوم |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4470 |
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| author | Duha Bayram Hayder Zuhair Ali |
| author_facet | Duha Bayram Hayder Zuhair Ali |
| author_sort | Duha Bayram |
| collection | DOAJ |
| description | Leishmaniasis is a group of parasitic diseases caused by Leishmania spp., an endemic infectious agent in developing countries, including Iraq. Diagnosis of cutaneous lesion by stained smears, serology or histopathology are inaccurate and unable to detect the species of Leishmania. Here, two molecular typing methods were examined to identify the promastigotes of suspected cutaneous leishmaniasis samples, on a species level. The first was species-specific B6-PCR and the second was ITS1-PCR followed by restriction fragment length polymorphism (RFLP) using restriction enzyme HaeIII. DNA was extracted from in vitro promastigote culture followed by amplification of kDNA by B6 or amplification and digestion of LITSR/L5.8S. PCR produced bands of ~359 bp and ~450 bp for B6 and ITS1, respectively. Digestion of ITS1 by RFLP revealed two distinct bands of ~150 bp and ~300 bp size. The results reviled that the two isolates belong to cutaneous Leishmaniasis, specifically Leishmania tropica. In conclusion, the confirmation of the studied methods will improve rapid and accurate diagnosis of Leishmania species of the most prevalent Iraqi strain of cutaneous leishmaniasis, L. tropica. |
| format | Article |
| id | doaj-art-aeef9a4166f34979ae36422df57db293 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-aeef9a4166f34979ae36422df57db2932025-08-20T03:20:27ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862021-03-0118110.21123/bsj.2021.18.1.0029Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in BaghdadDuha Bayram0Hayder Zuhair Ali1University of BaghdadUniversity of BaghdadLeishmaniasis is a group of parasitic diseases caused by Leishmania spp., an endemic infectious agent in developing countries, including Iraq. Diagnosis of cutaneous lesion by stained smears, serology or histopathology are inaccurate and unable to detect the species of Leishmania. Here, two molecular typing methods were examined to identify the promastigotes of suspected cutaneous leishmaniasis samples, on a species level. The first was species-specific B6-PCR and the second was ITS1-PCR followed by restriction fragment length polymorphism (RFLP) using restriction enzyme HaeIII. DNA was extracted from in vitro promastigote culture followed by amplification of kDNA by B6 or amplification and digestion of LITSR/L5.8S. PCR produced bands of ~359 bp and ~450 bp for B6 and ITS1, respectively. Digestion of ITS1 by RFLP revealed two distinct bands of ~150 bp and ~300 bp size. The results reviled that the two isolates belong to cutaneous Leishmaniasis, specifically Leishmania tropica. In conclusion, the confirmation of the studied methods will improve rapid and accurate diagnosis of Leishmania species of the most prevalent Iraqi strain of cutaneous leishmaniasis, L. tropica.https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4470 |
| spellingShingle | Duha Bayram Hayder Zuhair Ali Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad مجلة بغداد للعلوم |
| title | Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad |
| title_full | Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad |
| title_fullStr | Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad |
| title_full_unstemmed | Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad |
| title_short | Molecular Typing of Two Suspected Cutaneous Leishmaniasis Isolates in Baghdad |
| title_sort | molecular typing of two suspected cutaneous leishmaniasis isolates in baghdad |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4470 |
| work_keys_str_mv | AT duhabayram moleculartypingoftwosuspectedcutaneousleishmaniasisisolatesinbaghdad AT hayderzuhairali moleculartypingoftwosuspectedcutaneousleishmaniasisisolatesinbaghdad |