Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers

Abstract The Tibetan Plateau (TP) glaciers are influenced by monsoon and westerlies. They are highly sensitive to climate change, with atmospheric nitrogen deposition significantly impacting microbial communities and functions. However, key uncertainties persist regarding biogeography and drivers of...

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Main Authors: Zhihao Zhang, Yongqin Liu, Weishu Zhao, Keshao Liu, Yuying Chen, Feng Wang, Guannan Mao, Mukan Ji
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61002-x
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author Zhihao Zhang
Yongqin Liu
Weishu Zhao
Keshao Liu
Yuying Chen
Feng Wang
Guannan Mao
Mukan Ji
author_facet Zhihao Zhang
Yongqin Liu
Weishu Zhao
Keshao Liu
Yuying Chen
Feng Wang
Guannan Mao
Mukan Ji
author_sort Zhihao Zhang
collection DOAJ
description Abstract The Tibetan Plateau (TP) glaciers are influenced by monsoon and westerlies. They are highly sensitive to climate change, with atmospheric nitrogen deposition significantly impacting microbial communities and functions. However, key uncertainties persist regarding biogeography and drivers of genes and microbial communities involved in nitrogen cycling. Here, we investigate the diversity and transcriptional activity of microbial communities and nitrogen-cycling genes using 85 metagenomes and 28 metatranscriptomes from the ablation zone of 21 TP glaciers. Our results show that over 90% of the glacial taxa possess the potential for nitrogen metabolism, with ~33% exhibiting transcriptional activity. Moreover, monsoon-dominated glaciers present greater microbial diversity and higher prevalence of nitrogen-fixing genes than westerlies-dominated glaciers, linked to higher temperatures. Comparatively, the latter show elevated genomic potential for nitrous oxide emissions, likely due to higher nitrate concentrations. These findings establish temperature-nitrogen co-regulation of microbial nitrogen transformations, critical for predicting climate feedback in the extreme environment.
format Article
id doaj-art-aee9b2c6f76e46b8a02e7e074c5286a2
institution Kabale University
issn 2041-1723
language English
publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-aee9b2c6f76e46b8a02e7e074c5286a22025-08-20T03:45:31ZengNature PortfolioNature Communications2041-17232025-07-0116111510.1038/s41467-025-61002-xDistinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciersZhihao Zhang0Yongqin Liu1Weishu Zhao2Keshao Liu3Yuying Chen4Feng Wang5Guannan Mao6Mukan Ji7State Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of SciencesState Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of SciencesState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of SciencesCenter for the Pan-third Pole Environment, Lanzhou UniversityState Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of SciencesCenter for the Pan-third Pole Environment, Lanzhou UniversityCenter for the Pan-third Pole Environment, Lanzhou UniversityAbstract The Tibetan Plateau (TP) glaciers are influenced by monsoon and westerlies. They are highly sensitive to climate change, with atmospheric nitrogen deposition significantly impacting microbial communities and functions. However, key uncertainties persist regarding biogeography and drivers of genes and microbial communities involved in nitrogen cycling. Here, we investigate the diversity and transcriptional activity of microbial communities and nitrogen-cycling genes using 85 metagenomes and 28 metatranscriptomes from the ablation zone of 21 TP glaciers. Our results show that over 90% of the glacial taxa possess the potential for nitrogen metabolism, with ~33% exhibiting transcriptional activity. Moreover, monsoon-dominated glaciers present greater microbial diversity and higher prevalence of nitrogen-fixing genes than westerlies-dominated glaciers, linked to higher temperatures. Comparatively, the latter show elevated genomic potential for nitrous oxide emissions, likely due to higher nitrate concentrations. These findings establish temperature-nitrogen co-regulation of microbial nitrogen transformations, critical for predicting climate feedback in the extreme environment.https://doi.org/10.1038/s41467-025-61002-x
spellingShingle Zhihao Zhang
Yongqin Liu
Weishu Zhao
Keshao Liu
Yuying Chen
Feng Wang
Guannan Mao
Mukan Ji
Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
Nature Communications
title Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
title_full Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
title_fullStr Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
title_full_unstemmed Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
title_short Distinct genes and microbial communities involved in nitrogen cycling between monsoon- and westerlies-dominated Tibetan glaciers
title_sort distinct genes and microbial communities involved in nitrogen cycling between monsoon and westerlies dominated tibetan glaciers
url https://doi.org/10.1038/s41467-025-61002-x
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