Hydrolysis of lactose with -D-galactosidase

The conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactos...

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Main Authors: Vesna Stehlik-Tomas, Slobodan Grba, Damir Stanzer, Vlatka Gulan-Zetić
Format: Article
Language:English
Published: Croatian Dairy Union 2001-06-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=3555
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author Vesna Stehlik-Tomas
Slobodan Grba
Damir Stanzer
Vlatka Gulan-Zetić
author_facet Vesna Stehlik-Tomas
Slobodan Grba
Damir Stanzer
Vlatka Gulan-Zetić
author_sort Vesna Stehlik-Tomas
collection DOAJ
description The conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactose concentrations in medium and different concentrations of added enzyme. The results show that optimal temperature for hydrolysis was 40°C. The optimal amount of enzyme preparation was 2 gL-1 in lactose medium with 5-10 % lactose.
format Article
id doaj-art-aee5a1c0cbbb4f22b5758d700e28c3b5
institution Kabale University
issn 0026-704X
1846-4025
language English
publishDate 2001-06-01
publisher Croatian Dairy Union
record_format Article
series Mljekarstvo
spelling doaj-art-aee5a1c0cbbb4f22b5758d700e28c3b52025-08-20T03:39:04ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252001-06-01513187196Hydrolysis of lactose with -D-galactosidaseVesna Stehlik-TomasSlobodan GrbaDamir StanzerVlatka Gulan-ZetićThe conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactose concentrations in medium and different concentrations of added enzyme. The results show that optimal temperature for hydrolysis was 40°C. The optimal amount of enzyme preparation was 2 gL-1 in lactose medium with 5-10 % lactose.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=3555Saccharomyces cerevisiaewheyhidrolysis lactose
spellingShingle Vesna Stehlik-Tomas
Slobodan Grba
Damir Stanzer
Vlatka Gulan-Zetić
Hydrolysis of lactose with -D-galactosidase
Mljekarstvo
Saccharomyces cerevisiae
whey
hidrolysis lactose
title Hydrolysis of lactose with -D-galactosidase
title_full Hydrolysis of lactose with -D-galactosidase
title_fullStr Hydrolysis of lactose with -D-galactosidase
title_full_unstemmed Hydrolysis of lactose with -D-galactosidase
title_short Hydrolysis of lactose with -D-galactosidase
title_sort hydrolysis of lactose with d galactosidase
topic Saccharomyces cerevisiae
whey
hidrolysis lactose
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=3555
work_keys_str_mv AT vesnastehliktomas hydrolysisoflactosewithdgalactosidase
AT slobodangrba hydrolysisoflactosewithdgalactosidase
AT damirstanzer hydrolysisoflactosewithdgalactosidase
AT vlatkagulanzetic hydrolysisoflactosewithdgalactosidase