An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars
This study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) and Nishika Canal Sealer BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial root canals of the upper first molars were pulp...
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2025-01-01
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| Series: | Journal of Functional Biomaterials |
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| author | Shintaro Takahara Naoki Edanami Razi Saifullah Ibn Belal Kunihiko Yoshiba Shoji Takenaka Naoto Ohkura Nagako Yoshiba Susan Gomez-Kasimoto Yuichiro Noiri |
| author_facet | Shintaro Takahara Naoki Edanami Razi Saifullah Ibn Belal Kunihiko Yoshiba Shoji Takenaka Naoto Ohkura Nagako Yoshiba Susan Gomez-Kasimoto Yuichiro Noiri |
| author_sort | Shintaro Takahara |
| collection | DOAJ |
| description | This study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) and Nishika Canal Sealer BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial root canals of the upper first molars were pulpectomized and overfilled with EBC, NBG, or, as reference, epoxy resin-based AH Plus (AHP). After 28 days, periapical tissue reactions were assessed using microcomputed tomography and histological staining. The elemental composition and chemical composition of the extruded EBC and NBG were analyzed at Day 1 and 28 using an electron probe microanalyzer and micro-Raman spectroscopy. No periapical lesions were observed with the sealer extrusion. Additionally, inflammation around the extruded EBC and NBG was minimal to mild on Day 28, whereas moderate inflammation was found around the extruded AHP. Silicon concentration in the extruded EBC and NBG decreased significantly from Day 1 to 28, with almost no silicon present on Day 28. Furthermore, the extruded EBC and NBG became calcium- and phosphorus-rich, showing a Raman band for hydroxyapatite on Day 28. In conclusion, EBC and NBG demonstrated favorable biocompatibility and the ability to release silicon elements and produce hydroxyapatite when extruded into the periapical tissues. AHP showed moderate periapical tissue irritancy. |
| format | Article |
| id | doaj-art-aed9841759034e3a806583128af0e10f |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-aed9841759034e3a806583128af0e10f2025-01-24T13:36:07ZengMDPI AGJournal of Functional Biomaterials2079-49832025-01-011611410.3390/jfb16010014An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat MolarsShintaro Takahara0Naoki Edanami1Razi Saifullah Ibn Belal2Kunihiko Yoshiba3Shoji Takenaka4Naoto Ohkura5Nagako Yoshiba6Susan Gomez-Kasimoto7Yuichiro Noiri8Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Oral Science for Health Promotion, Department of Oral Health and Welfare, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Oral Science for Health Promotion, Department of Oral Health and Welfare, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8126, JapanThis study assessed the biocompatibility and chemical properties of two bioceramic root canal sealers, EndoSequence BC Sealer (EBC) and Nishika Canal Sealer BG (NBG), using a sealer extrusion model. Eight-week-old male Wistar rats were used. The mesial root canals of the upper first molars were pulpectomized and overfilled with EBC, NBG, or, as reference, epoxy resin-based AH Plus (AHP). After 28 days, periapical tissue reactions were assessed using microcomputed tomography and histological staining. The elemental composition and chemical composition of the extruded EBC and NBG were analyzed at Day 1 and 28 using an electron probe microanalyzer and micro-Raman spectroscopy. No periapical lesions were observed with the sealer extrusion. Additionally, inflammation around the extruded EBC and NBG was minimal to mild on Day 28, whereas moderate inflammation was found around the extruded AHP. Silicon concentration in the extruded EBC and NBG decreased significantly from Day 1 to 28, with almost no silicon present on Day 28. Furthermore, the extruded EBC and NBG became calcium- and phosphorus-rich, showing a Raman band for hydroxyapatite on Day 28. In conclusion, EBC and NBG demonstrated favorable biocompatibility and the ability to release silicon elements and produce hydroxyapatite when extruded into the periapical tissues. AHP showed moderate periapical tissue irritancy.https://www.mdpi.com/2079-4983/16/1/14bioceramic root canal sealersealer extrusionbiocompatibilitysilicon element releasehydroxyapatite formation |
| spellingShingle | Shintaro Takahara Naoki Edanami Razi Saifullah Ibn Belal Kunihiko Yoshiba Shoji Takenaka Naoto Ohkura Nagako Yoshiba Susan Gomez-Kasimoto Yuichiro Noiri An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars Journal of Functional Biomaterials bioceramic root canal sealer sealer extrusion biocompatibility silicon element release hydroxyapatite formation |
| title | An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars |
| title_full | An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars |
| title_fullStr | An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars |
| title_full_unstemmed | An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars |
| title_short | An Evaluation of the Biocompatibility and Chemical Properties of Two Bioceramic Root Canal Sealers in a Sealer Extrusion Model of Rat Molars |
| title_sort | evaluation of the biocompatibility and chemical properties of two bioceramic root canal sealers in a sealer extrusion model of rat molars |
| topic | bioceramic root canal sealer sealer extrusion biocompatibility silicon element release hydroxyapatite formation |
| url | https://www.mdpi.com/2079-4983/16/1/14 |
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