Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities
Antarctic krill (<i>Euphausia superba</i>) is a nutrient-rich marine resource. Although several terrestrial proteases have been used to prepare Antarctic krill peptides (AKPs), there has been no report on the preparation of AKPs using a marine protease. Here, marine bacterial protease A6...
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MDPI AG
2025-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/23/6/226 |
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| author | Rui Liu Wen-Jie Cao Wen-Xiao Zhao Xiao-Jie Yuan Yu-Zhong Zhang Qi-Long Qin Xiao-Yan Song Xi-Ying Zhang Jian Li Xiu-Lan Chen Yu-Qiang Zhang |
| author_facet | Rui Liu Wen-Jie Cao Wen-Xiao Zhao Xiao-Jie Yuan Yu-Zhong Zhang Qi-Long Qin Xiao-Yan Song Xi-Ying Zhang Jian Li Xiu-Lan Chen Yu-Qiang Zhang |
| author_sort | Rui Liu |
| collection | DOAJ |
| description | Antarctic krill (<i>Euphausia superba</i>) is a nutrient-rich marine resource. Although several terrestrial proteases have been used to prepare Antarctic krill peptides (AKPs), there has been no report on the preparation of AKPs using a marine protease. Here, marine bacterial protease A69 was used to prepare AKPs with multi-bioactivities. Through optimizing hydrolysis parameters, we established a process for AKPs preparation by hydrolyzing Antarctic krill powder with A69. In the prepared AKPs, peptides less than 3000 Da and 1000 Da accounted for 99.23% and 88.37%, respectively. The scavenging ratios of the AKPs to ABTS<sup>+</sup>, DPPH· and ·OH reached 93.23 ± 0.09%, 99.90 ± 0.15%, and 93.90 ± 0.47%, respectively. The AKPs also had high angiotensin-converting enzyme (ACE)-inhibitory activity, with an IC<sub>50</sub> of 0.22 ± 0.04 mg/mL. At 40 mg/mL, the AKPs inhibited α-glucosidase and dipeptidyl peptidase IV (DPP-IV) activities by 7.18% and 13.62%, respectively, and displayed antibacterial activity to <i>Escherichia coli</i>. Moreover, 14 antioxidant peptides, 24 ACE-inhibitory peptides, 2 α-glucosidase-inhibitory peptides, and 10 DPP-Ⅳ-inhibitory peptides were identified from the AKPs. These results demonstrate that the prepared AKPs contain diverse bioactive peptides and have multi-bioactivities. This study indicates that marine bacterial protease A69 has promising application potential in preparing AKPs with multi-bioactivities. |
| format | Article |
| id | doaj-art-aece7b20d4124267b57193c05638dba2 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-aece7b20d4124267b57193c05638dba22025-08-20T03:27:30ZengMDPI AGMarine Drugs1660-33972025-05-0123622610.3390/md23060226Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-BioactivitiesRui Liu0Wen-Jie Cao1Wen-Xiao Zhao2Xiao-Jie Yuan3Yu-Zhong Zhang4Qi-Long Qin5Xiao-Yan Song6Xi-Ying Zhang7Jian Li8Xiu-Lan Chen9Yu-Qiang Zhang10State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, ChinaAntarctic krill (<i>Euphausia superba</i>) is a nutrient-rich marine resource. Although several terrestrial proteases have been used to prepare Antarctic krill peptides (AKPs), there has been no report on the preparation of AKPs using a marine protease. Here, marine bacterial protease A69 was used to prepare AKPs with multi-bioactivities. Through optimizing hydrolysis parameters, we established a process for AKPs preparation by hydrolyzing Antarctic krill powder with A69. In the prepared AKPs, peptides less than 3000 Da and 1000 Da accounted for 99.23% and 88.37%, respectively. The scavenging ratios of the AKPs to ABTS<sup>+</sup>, DPPH· and ·OH reached 93.23 ± 0.09%, 99.90 ± 0.15%, and 93.90 ± 0.47%, respectively. The AKPs also had high angiotensin-converting enzyme (ACE)-inhibitory activity, with an IC<sub>50</sub> of 0.22 ± 0.04 mg/mL. At 40 mg/mL, the AKPs inhibited α-glucosidase and dipeptidyl peptidase IV (DPP-IV) activities by 7.18% and 13.62%, respectively, and displayed antibacterial activity to <i>Escherichia coli</i>. Moreover, 14 antioxidant peptides, 24 ACE-inhibitory peptides, 2 α-glucosidase-inhibitory peptides, and 10 DPP-Ⅳ-inhibitory peptides were identified from the AKPs. These results demonstrate that the prepared AKPs contain diverse bioactive peptides and have multi-bioactivities. This study indicates that marine bacterial protease A69 has promising application potential in preparing AKPs with multi-bioactivities.https://www.mdpi.com/1660-3397/23/6/226marine bacterial protease A69Antarctic krill peptidesantioxidant activityangiotensin-converting enzyme (ACE)-inhibitory activityantibacterial activityα-glucosidase-inhibitory activity |
| spellingShingle | Rui Liu Wen-Jie Cao Wen-Xiao Zhao Xiao-Jie Yuan Yu-Zhong Zhang Qi-Long Qin Xiao-Yan Song Xi-Ying Zhang Jian Li Xiu-Lan Chen Yu-Qiang Zhang Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities Marine Drugs marine bacterial protease A69 Antarctic krill peptides antioxidant activity angiotensin-converting enzyme (ACE)-inhibitory activity antibacterial activity α-glucosidase-inhibitory activity |
| title | Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities |
| title_full | Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities |
| title_fullStr | Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities |
| title_full_unstemmed | Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities |
| title_short | Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities |
| title_sort | potential of marine bacterial metalloprotease a69 in the preparation of antarctic krill peptides with multi bioactivities |
| topic | marine bacterial protease A69 Antarctic krill peptides antioxidant activity angiotensin-converting enzyme (ACE)-inhibitory activity antibacterial activity α-glucosidase-inhibitory activity |
| url | https://www.mdpi.com/1660-3397/23/6/226 |
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