Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling
The TGF-β signaling pathway is closely associated with human health and disease, and the systematic identification of factors involved in the TGF-β signaling pathway significantly contributes to the understanding and treatment of various diseases. Through kinome-wide CRISPR screen, we identified 13...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580824002280 |
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| author | Dingding Wang Xinhao Zhang Jianxun Guo Weijia Liu Yanchi Zhou Renxian Wang |
| author_facet | Dingding Wang Xinhao Zhang Jianxun Guo Weijia Liu Yanchi Zhou Renxian Wang |
| author_sort | Dingding Wang |
| collection | DOAJ |
| description | The TGF-β signaling pathway is closely associated with human health and disease, and the systematic identification of factors involved in the TGF-β signaling pathway significantly contributes to the understanding and treatment of various diseases. Through kinome-wide CRISPR screen, we identified 13 candidate regulatory targets. Notably, the well-known hallmark genes TGFBR1 and TGFBR2 emerged as the top two candidate targets. OXSR1 and EXOSC10 were ranked third and fourth as positive candidate targets, respectively, with EXOSC10 being a novel discovery. Importantly, our findings revealed the down-regulation of OXSR1 and EXOSC10 using CRISPR knockout and RNAi technology effectively suppressed the TGF-β signaling pathway in HeLa and HaCaT cells, providing new insights of TGF-β signaling. |
| format | Article |
| id | doaj-art-aecbb43694934f1ab4f7ac48b6dfb829 |
| institution | OA Journals |
| issn | 2405-5808 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-aecbb43694934f1ab4f7ac48b6dfb8292025-08-20T02:19:51ZengElsevierBiochemistry and Biophysics Reports2405-58082024-12-014010186410.1016/j.bbrep.2024.101864Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signalingDingding Wang0Xinhao Zhang1Jianxun Guo2Weijia Liu3Yanchi Zhou4Renxian Wang5JST Sarcopenia Research Centre, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, ChinaDepartment of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China; Center for Bioinformatics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100005, ChinaJST Sarcopenia Research Centre, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, ChinaJST Sarcopenia Research Centre, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, ChinaDepartment of Hepatobiliary Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, ChinaJST Sarcopenia Research Centre, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, 100035, ChinaThe TGF-β signaling pathway is closely associated with human health and disease, and the systematic identification of factors involved in the TGF-β signaling pathway significantly contributes to the understanding and treatment of various diseases. Through kinome-wide CRISPR screen, we identified 13 candidate regulatory targets. Notably, the well-known hallmark genes TGFBR1 and TGFBR2 emerged as the top two candidate targets. OXSR1 and EXOSC10 were ranked third and fourth as positive candidate targets, respectively, with EXOSC10 being a novel discovery. Importantly, our findings revealed the down-regulation of OXSR1 and EXOSC10 using CRISPR knockout and RNAi technology effectively suppressed the TGF-β signaling pathway in HeLa and HaCaT cells, providing new insights of TGF-β signaling.http://www.sciencedirect.com/science/article/pii/S2405580824002280TGF-β signalingEXOSC10Kinome-wide CRISPR screen |
| spellingShingle | Dingding Wang Xinhao Zhang Jianxun Guo Weijia Liu Yanchi Zhou Renxian Wang Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling Biochemistry and Biophysics Reports TGF-β signaling EXOSC10 Kinome-wide CRISPR screen |
| title | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
| title_full | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
| title_fullStr | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
| title_full_unstemmed | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
| title_short | Kinome-wide CRISPR-Cas9 screens revealed EXOSC10 as a positive regulator of TGF-β signaling |
| title_sort | kinome wide crispr cas9 screens revealed exosc10 as a positive regulator of tgf β signaling |
| topic | TGF-β signaling EXOSC10 Kinome-wide CRISPR screen |
| url | http://www.sciencedirect.com/science/article/pii/S2405580824002280 |
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