Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα
Renal ischemia-reperfusion (I/R) can induce oxidative stress and injury via the generation of reactive oxygen species (ROS). Renal proximal tubular cells are susceptible to oxidative stress, and the dysregulation of renal proximal tubular cellular homeostasis can damage cells via apoptotic pathways....
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/9972704 |
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| author | Sujuan Xu Edward Lee Zhaoxing Sun Xiaoyan Wang Ting Ren Zhouping Zou Jifu Jin Jie Li Jian Zhang Yingxiang Li Qiang Yang Yang Zhang Man Guo Yi Fang Xiaoqiang Ding |
| author_facet | Sujuan Xu Edward Lee Zhaoxing Sun Xiaoyan Wang Ting Ren Zhouping Zou Jifu Jin Jie Li Jian Zhang Yingxiang Li Qiang Yang Yang Zhang Man Guo Yi Fang Xiaoqiang Ding |
| author_sort | Sujuan Xu |
| collection | DOAJ |
| description | Renal ischemia-reperfusion (I/R) can induce oxidative stress and injury via the generation of reactive oxygen species (ROS). Renal proximal tubular cells are susceptible to oxidative stress, and the dysregulation of renal proximal tubular cellular homeostasis can damage cells via apoptotic pathways. A recent study showed that the generation of ROS can increase perilipin 2 (Plin2) expression in HepG2 cells. Some evidence has also demonstrated the association between Plin2 expression and renal tumors. However, the underlying mechanism of Plin2 in I/R-induced acute kidney injury (AKI) remains elusive. Here, using a mouse model of I/R-induced AKI, we found that ROS generation was increased and the expression of Plin2 was significantly upregulated. An in vitro study further revealed that the expression of Plin2, and the generation of ROS were significantly upregulated in primary tubular cells treated with hydrogen peroxide. Accordingly, Plin2 knockdown decreased apoptosis in renal proximal tubular epithelial cells treated with hydrogen peroxide, which depended on the activation of peroxisome proliferator-activated receptor α (PPARα). Overall, the present study demonstrated that Plin2 is involved in AKI; knockdown of this marker might limit apoptosis via the activation of PPARα. Consequently, the downregulation of Plin2 could be a novel therapeutic strategy for AKI. |
| format | Article |
| id | doaj-art-aec8f3ad60574bc2bc8341777ea34e7c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-aec8f3ad60574bc2bc8341777ea34e7c2025-02-03T01:25:48ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/99727049972704Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARαSujuan Xu0Edward Lee1Zhaoxing Sun2Xiaoyan Wang3Ting Ren4Zhouping Zou5Jifu Jin6Jie Li7Jian Zhang8Yingxiang Li9Qiang Yang10Yang Zhang11Man Guo12Yi Fang13Xiaoqiang Ding14Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, ChinaRenal ischemia-reperfusion (I/R) can induce oxidative stress and injury via the generation of reactive oxygen species (ROS). Renal proximal tubular cells are susceptible to oxidative stress, and the dysregulation of renal proximal tubular cellular homeostasis can damage cells via apoptotic pathways. A recent study showed that the generation of ROS can increase perilipin 2 (Plin2) expression in HepG2 cells. Some evidence has also demonstrated the association between Plin2 expression and renal tumors. However, the underlying mechanism of Plin2 in I/R-induced acute kidney injury (AKI) remains elusive. Here, using a mouse model of I/R-induced AKI, we found that ROS generation was increased and the expression of Plin2 was significantly upregulated. An in vitro study further revealed that the expression of Plin2, and the generation of ROS were significantly upregulated in primary tubular cells treated with hydrogen peroxide. Accordingly, Plin2 knockdown decreased apoptosis in renal proximal tubular epithelial cells treated with hydrogen peroxide, which depended on the activation of peroxisome proliferator-activated receptor α (PPARα). Overall, the present study demonstrated that Plin2 is involved in AKI; knockdown of this marker might limit apoptosis via the activation of PPARα. Consequently, the downregulation of Plin2 could be a novel therapeutic strategy for AKI.http://dx.doi.org/10.1155/2021/9972704 |
| spellingShingle | Sujuan Xu Edward Lee Zhaoxing Sun Xiaoyan Wang Ting Ren Zhouping Zou Jifu Jin Jie Li Jian Zhang Yingxiang Li Qiang Yang Yang Zhang Man Guo Yi Fang Xiaoqiang Ding Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα Journal of Immunology Research |
| title | Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα |
| title_full | Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα |
| title_fullStr | Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα |
| title_full_unstemmed | Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα |
| title_short | Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα |
| title_sort | perilipin 2 impacts acute kidney injury via regulation of pparα |
| url | http://dx.doi.org/10.1155/2021/9972704 |
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