Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil.
<h4>Background</h4>The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that c...
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Public Library of Science (PLoS)
2016-10-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005048&type=printable |
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| author | Claire L Donald Benjamin Brennan Stephanie L Cumberworth Veronica V Rezelj Jordan J Clark Marli T Cordeiro Rafael Freitas de Oliveira França Lindomar J Pena Gavin S Wilkie Ana Da Silva Filipe Christopher Davis Joseph Hughes Margus Varjak Martin Selinger Luíza Zuvanov Ania M Owsianka Arvind H Patel John McLauchlan Brett D Lindenbach Gamou Fall Amadou A Sall Roman Biek Jan Rehwinkel Esther Schnettler Alain Kohl |
| author_facet | Claire L Donald Benjamin Brennan Stephanie L Cumberworth Veronica V Rezelj Jordan J Clark Marli T Cordeiro Rafael Freitas de Oliveira França Lindomar J Pena Gavin S Wilkie Ana Da Silva Filipe Christopher Davis Joseph Hughes Margus Varjak Martin Selinger Luíza Zuvanov Ania M Owsianka Arvind H Patel John McLauchlan Brett D Lindenbach Gamou Fall Amadou A Sall Roman Biek Jan Rehwinkel Esther Schnettler Alain Kohl |
| author_sort | Claire L Donald |
| collection | DOAJ |
| description | <h4>Background</h4>The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that contribute to the associated pathogenesis. Determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative processes and virus-host interactions.<h4>Methodology/principal findings</h4>We obtained a ZIKV isolate from a patient who presented with classical ZIKV-associated symptoms, and used high throughput sequencing and other molecular biology approaches to determine its full genome sequence, including non-coding regions. Genome regions were characterized and compared to the sequences of other isolates where available. Furthermore, we identified a subgenomic flavivirus RNA (sfRNA) in ZIKV-infected cells that has antagonist activity against RIG-I induced type I interferon induction, with a lesser effect on MDA-5 mediated action.<h4>Conclusions/significance</h4>The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus. Detection of sfRNA that counteracts interferon responses is likely to be important for further understanding of pathogenesis and virus-host interactions. |
| format | Article |
| id | doaj-art-aec56e53090642d5b80da2d66a065408 |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-aec56e53090642d5b80da2d66a0654082025-08-20T02:03:23ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-10-011010e000504810.1371/journal.pntd.0005048Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil.Claire L DonaldBenjamin BrennanStephanie L CumberworthVeronica V RezeljJordan J ClarkMarli T CordeiroRafael Freitas de Oliveira FrançaLindomar J PenaGavin S WilkieAna Da Silva FilipeChristopher DavisJoseph HughesMargus VarjakMartin SelingerLuíza ZuvanovAnia M OwsiankaArvind H PatelJohn McLauchlanBrett D LindenbachGamou FallAmadou A SallRoman BiekJan RehwinkelEsther SchnettlerAlain Kohl<h4>Background</h4>The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that contribute to the associated pathogenesis. Determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative processes and virus-host interactions.<h4>Methodology/principal findings</h4>We obtained a ZIKV isolate from a patient who presented with classical ZIKV-associated symptoms, and used high throughput sequencing and other molecular biology approaches to determine its full genome sequence, including non-coding regions. Genome regions were characterized and compared to the sequences of other isolates where available. Furthermore, we identified a subgenomic flavivirus RNA (sfRNA) in ZIKV-infected cells that has antagonist activity against RIG-I induced type I interferon induction, with a lesser effect on MDA-5 mediated action.<h4>Conclusions/significance</h4>The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus. Detection of sfRNA that counteracts interferon responses is likely to be important for further understanding of pathogenesis and virus-host interactions.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005048&type=printable |
| spellingShingle | Claire L Donald Benjamin Brennan Stephanie L Cumberworth Veronica V Rezelj Jordan J Clark Marli T Cordeiro Rafael Freitas de Oliveira França Lindomar J Pena Gavin S Wilkie Ana Da Silva Filipe Christopher Davis Joseph Hughes Margus Varjak Martin Selinger Luíza Zuvanov Ania M Owsianka Arvind H Patel John McLauchlan Brett D Lindenbach Gamou Fall Amadou A Sall Roman Biek Jan Rehwinkel Esther Schnettler Alain Kohl Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. PLoS Neglected Tropical Diseases |
| title | Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. |
| title_full | Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. |
| title_fullStr | Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. |
| title_full_unstemmed | Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. |
| title_short | Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil. |
| title_sort | full genome sequence and sfrna interferon antagonist activity of zika virus from recife brazil |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005048&type=printable |
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