Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity
Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. Here, we describe a protocol for differentiating hiPSCs into thalamic and cortical organoids and then fusing the...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500036X |
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| author | Anjana Nityanandam Mary H. Patton Ildar T. Bayazitov Kyle D. Newman Kristen T. Thomas Stanislav S. Zakharenko |
| author_facet | Anjana Nityanandam Mary H. Patton Ildar T. Bayazitov Kyle D. Newman Kristen T. Thomas Stanislav S. Zakharenko |
| author_sort | Anjana Nityanandam |
| collection | DOAJ |
| description | Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. Here, we describe a protocol for differentiating hiPSCs into thalamic and cortical organoids and then fusing them to generate thalamocortical assembloids. We detail the steps for using whole-cell patch-clamp electrophysiology to investigate the properties of synaptic transmission and synaptic plasticity in this model system.For complete details on the use and execution of this protocol, please refer to Patton et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-aebcf53ad3b64528adb6594cd08654b8 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-aebcf53ad3b64528adb6594cd08654b82025-02-09T05:01:24ZengElsevierSTAR Protocols2666-16672025-03-0161103630Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticityAnjana Nityanandam0Mary H. Patton1Ildar T. Bayazitov2Kyle D. Newman3Kristen T. Thomas4Stanislav S. Zakharenko5Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA; Corresponding authorDepartment of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA; Corresponding authorSummary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. Here, we describe a protocol for differentiating hiPSCs into thalamic and cortical organoids and then fusing them to generate thalamocortical assembloids. We detail the steps for using whole-cell patch-clamp electrophysiology to investigate the properties of synaptic transmission and synaptic plasticity in this model system.For complete details on the use and execution of this protocol, please refer to Patton et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672500036XNeuroscienceStem CellsOrganoids |
| spellingShingle | Anjana Nityanandam Mary H. Patton Ildar T. Bayazitov Kyle D. Newman Kristen T. Thomas Stanislav S. Zakharenko Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity STAR Protocols Neuroscience Stem Cells Organoids |
| title | Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| title_full | Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| title_fullStr | Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| title_full_unstemmed | Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| title_short | Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| title_sort | protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity |
| topic | Neuroscience Stem Cells Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S266616672500036X |
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