Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies
Abstract Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-th...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-03-01
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| Series: | Cell Regeneration |
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| Online Access: | https://doi.org/10.1186/s13619-025-00227-z |
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| author | Xing Hu Baoqiang Kang Mingquan Wang Huaisong Lin Zhiyong Liu Zhishuai Zhang Jiaming Gu Yuchan Mai Xinrui Guo Wanli Ma Han Yan Shuoting Wang Jingxi Huang Junwei Wang Jian Zhang Tianyu Zhang Bo Feng Yanling Zhu Guangjin Pan |
| author_facet | Xing Hu Baoqiang Kang Mingquan Wang Huaisong Lin Zhiyong Liu Zhishuai Zhang Jiaming Gu Yuchan Mai Xinrui Guo Wanli Ma Han Yan Shuoting Wang Jingxi Huang Junwei Wang Jian Zhang Tianyu Zhang Bo Feng Yanling Zhu Guangjin Pan |
| author_sort | Xing Hu |
| collection | DOAJ |
| description | Abstract Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented. Here, we develop a scalable approach to generate iNEUs in a chemical defined condition. iNEUs display typical neutrophil characters in terms of phagocytosis, migration, formation of neutrophil extracellular traps (NETs), etc. Importantly, iNEUs display a strong killing potency against various bacteria such as K.pneumoniae, P.aeruginosa, E.coli and S.aureus. Moreover, transfusions of iNEUs in mice with neutrophil dysfunction largely enhance their survival in lethal infection of different bacteria. Together, our data show that hiPSCs derived neutrophils hold strong anti-microbial potencies to protect severe infections under neutrophil dysfunction conditions. |
| format | Article |
| id | doaj-art-aebb91d2e855404db770ce391b80223d |
| institution | Kabale University |
| issn | 2045-9769 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj-art-aebb91d2e855404db770ce391b80223d2025-08-20T04:03:03ZengSpringerOpenCell Regeneration2045-97692025-03-0114111410.1186/s13619-025-00227-zHuman induced pluripotent stem cells derived neutrophils display strong anti-microbial potenciesXing Hu0Baoqiang Kang1Mingquan Wang2Huaisong Lin3Zhiyong Liu4Zhishuai Zhang5Jiaming Gu6Yuchan Mai7Xinrui Guo8Wanli Ma9Han Yan10Shuoting Wang11Jingxi Huang12Junwei Wang13Jian Zhang14Tianyu Zhang15Bo Feng16Yanling Zhu17Guangjin Pan18National Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCentre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, Chinese Academy of SciencesCentre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesThe Third Affiliated Hospital of Sun Yat-Sen UniversityNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesSchool of Biomedical Sciences, Faculty of Medicine, CUHK-GIBH CAS Joint Research Laboratory on Stem Cell and Regenerative Medicine, The Chinese University of Hong KongNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNational Key Laboratory of Immune Response and Immunotherapy, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesAbstract Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented. Here, we develop a scalable approach to generate iNEUs in a chemical defined condition. iNEUs display typical neutrophil characters in terms of phagocytosis, migration, formation of neutrophil extracellular traps (NETs), etc. Importantly, iNEUs display a strong killing potency against various bacteria such as K.pneumoniae, P.aeruginosa, E.coli and S.aureus. Moreover, transfusions of iNEUs in mice with neutrophil dysfunction largely enhance their survival in lethal infection of different bacteria. Together, our data show that hiPSCs derived neutrophils hold strong anti-microbial potencies to protect severe infections under neutrophil dysfunction conditions.https://doi.org/10.1186/s13619-025-00227-zhiPSCsNeutrophilsBacteriaCell therapy |
| spellingShingle | Xing Hu Baoqiang Kang Mingquan Wang Huaisong Lin Zhiyong Liu Zhishuai Zhang Jiaming Gu Yuchan Mai Xinrui Guo Wanli Ma Han Yan Shuoting Wang Jingxi Huang Junwei Wang Jian Zhang Tianyu Zhang Bo Feng Yanling Zhu Guangjin Pan Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies Cell Regeneration hiPSCs Neutrophils Bacteria Cell therapy |
| title | Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies |
| title_full | Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies |
| title_fullStr | Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies |
| title_full_unstemmed | Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies |
| title_short | Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies |
| title_sort | human induced pluripotent stem cells derived neutrophils display strong anti microbial potencies |
| topic | hiPSCs Neutrophils Bacteria Cell therapy |
| url | https://doi.org/10.1186/s13619-025-00227-z |
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