Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test
Flavonoids are a group of polyphenolic compounds produced in plants as secondary metabolites. Flavonoids have favorable biochemical effects on multiple diseases and other bioactivities. This study aimed to isolate the active compounds contained in malacca (Phyllanthus emblica) leaves and analyze tox...
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Universitas Airlangga, Faculty of Veterinary Medicine
2023-12-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/46240 |
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| author | Muhammad Zakwan Amalia Sutriana Nurliana Nurliana Nuzul Asmilia Muhammad Ammar Arindita Niatazya Novianti |
| author_facet | Muhammad Zakwan Amalia Sutriana Nurliana Nurliana Nuzul Asmilia Muhammad Ammar Arindita Niatazya Novianti |
| author_sort | Muhammad Zakwan |
| collection | DOAJ |
| description | Flavonoids are a group of polyphenolic compounds produced in plants as secondary metabolites. Flavonoids have favorable biochemical effects on multiple diseases and other bioactivities. This study aimed to isolate the active compounds contained in malacca (Phyllanthus emblica) leaves and analyze toxic properties using the Brine Shrimp Lethality Test (BSLT) method in Artemia salina leach shrimp larvae. This study used malacca leaf powder with ethyl acetate solvent then macerated. Ethyl acetate extract was concentrated and fractionated, then toxicity tests were carried out. Extracts from the evaporation results were further fractionated using solvent petroleum ether, diethyl ether, and ethyl acetate. Then the ethyl acetate fraction was hydrolyzed using reflagged with 7% sulfuric acid for two hours, then the filtrate was extracted with ethanol solvent. The obtained fraction washed with aquades and dried using a vacuum desiccator. The toxicity test results showed that the flavonoid compound of malacca leaves was not toxic with a value of LC50 > 1000 ppm, an increase in the concentration of the extract was followed by an increase in the average mortality of larvae. |
| format | Article |
| id | doaj-art-aea5ff95244d4f4a9fa2b29a1dbcf522 |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-aea5ff95244d4f4a9fa2b29a1dbcf5222025-01-03T00:12:40ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2023-12-0163151910.20473/jmv.vol6.iss3.2023.15-1944326Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality TestMuhammad Zakwan0https://orcid.org/0000-0002-9269-514XAmalia Sutriana1https://orcid.org/0000-0002-6669-7491Nurliana Nurliana2https://orcid.org/0000-0003-4250-8753Nuzul Asmilia3https://orcid.org/0000-0003-0531-2049Muhammad Ammar4https://orcid.org/0000-0003-1455-8796Arindita Niatazya Novianti5https://orcid.org/0009-0002-3735-2686Veterinary Public Health Study Program, Faculty of Veterinary Medicine, Syiah Kuala University, Banda AcehLaboratory of Pharmacology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda AcehLaboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Syiah Kuala University, Banda AcehLaboratory of Clinic, Faculty of Veterinary Medicine, Syiah Kuala University, Banda AcehStudy Program of Veterinary Medicine, Faculty of Veterinary Medicine, Syiah Kuala University, Banda AcehDivision of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, SurabayaFlavonoids are a group of polyphenolic compounds produced in plants as secondary metabolites. Flavonoids have favorable biochemical effects on multiple diseases and other bioactivities. This study aimed to isolate the active compounds contained in malacca (Phyllanthus emblica) leaves and analyze toxic properties using the Brine Shrimp Lethality Test (BSLT) method in Artemia salina leach shrimp larvae. This study used malacca leaf powder with ethyl acetate solvent then macerated. Ethyl acetate extract was concentrated and fractionated, then toxicity tests were carried out. Extracts from the evaporation results were further fractionated using solvent petroleum ether, diethyl ether, and ethyl acetate. Then the ethyl acetate fraction was hydrolyzed using reflagged with 7% sulfuric acid for two hours, then the filtrate was extracted with ethanol solvent. The obtained fraction washed with aquades and dried using a vacuum desiccator. The toxicity test results showed that the flavonoid compound of malacca leaves was not toxic with a value of LC50 > 1000 ppm, an increase in the concentration of the extract was followed by an increase in the average mortality of larvae.https://e-journal.unair.ac.id/JMV/article/view/46240artemia salinabrine shrimp lethality testflavonoidsphyllanthus emblica |
| spellingShingle | Muhammad Zakwan Amalia Sutriana Nurliana Nurliana Nuzul Asmilia Muhammad Ammar Arindita Niatazya Novianti Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test Jurnal Medik Veteriner artemia salina brine shrimp lethality test flavonoids phyllanthus emblica |
| title | Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test |
| title_full | Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test |
| title_fullStr | Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test |
| title_full_unstemmed | Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test |
| title_short | Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test |
| title_sort | toxicity test of flavonoid compounds from ethyl acetate extract of malacca leaves with brine shrimp lethality test |
| topic | artemia salina brine shrimp lethality test flavonoids phyllanthus emblica |
| url | https://e-journal.unair.ac.id/JMV/article/view/46240 |
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