Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological di...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/760324 |
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| _version_ | 1850160517677580288 |
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| author | Àgnes Bànkfalvi Klaus Schmitz Tobias Mock Mechthild Kemper Christiane Cubick Werner Böcker |
| author_facet | Àgnes Bànkfalvi Klaus Schmitz Tobias Mock Mechthild Kemper Christiane Cubick Werner Böcker |
| author_sort | Àgnes Bànkfalvi |
| collection | DOAJ |
| description | The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological differentiation grade in serial paraffin sections from 39 breast carcinomas. Ten sections were double-stained for AgNOR/MIB1. AgNOR parameters correlated significantly with MIB1 growth fraction and p53 protein expression. Significant inverse correlation was found between proliferation markers and oestrogen/progesterone receptor status and histopathological grade. AgNOR expression was significantly higher in cycling (MIB1 positive) tumour cells, than in resting (MIB1 negative) ones, however with exceptions. We conclude, that standardised AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. However, AgNORs seem to reflect proliferation independent cellular and nucleolar activity of tumour cells, as well. We recommend the use of standardised AgNOR analysis for obtaining sound results in routine paraffin sections. |
| format | Article |
| id | doaj-art-ae9e73a8efa94337abbecfd8da0c0fb9 |
| institution | OA Journals |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-ae9e73a8efa94337abbecfd8da0c0fb92025-08-20T02:23:08ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0117423124210.1155/1998/760324Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast CarcinomasÀgnes Bànkfalvi0Klaus Schmitz1Tobias Mock2Mechthild Kemper3Christiane Cubick4Werner Böcker5Domagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyDomagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyDomagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyDomagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyDomagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyDomagk Institute of Pathology, University of Münster, Domagkstrasse 17, D-48149 Münster, GermanyThe present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological differentiation grade in serial paraffin sections from 39 breast carcinomas. Ten sections were double-stained for AgNOR/MIB1. AgNOR parameters correlated significantly with MIB1 growth fraction and p53 protein expression. Significant inverse correlation was found between proliferation markers and oestrogen/progesterone receptor status and histopathological grade. AgNOR expression was significantly higher in cycling (MIB1 positive) tumour cells, than in resting (MIB1 negative) ones, however with exceptions. We conclude, that standardised AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. However, AgNORs seem to reflect proliferation independent cellular and nucleolar activity of tumour cells, as well. We recommend the use of standardised AgNOR analysis for obtaining sound results in routine paraffin sections.http://dx.doi.org/10.1155/1998/760324 |
| spellingShingle | Àgnes Bànkfalvi Klaus Schmitz Tobias Mock Mechthild Kemper Christiane Cubick Werner Böcker Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas Analytical Cellular Pathology |
| title | Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas |
| title_full | Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas |
| title_fullStr | Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas |
| title_full_unstemmed | Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas |
| title_short | Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas |
| title_sort | relationship between agnor proteins ki 67 antigen p53 immunophenotype and differentiation markers in archival breast carcinomas |
| url | http://dx.doi.org/10.1155/1998/760324 |
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