Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts
Aim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors’ secretion (EGF, bFGF, and VEGF). Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the foll...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2015/258941 |
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| author | Andreas S. Gkogkos Ioannis K. Karoussis Ioannis D. Prevezanos Kleopatra E. Marcopoulou Kyriaki Kyriakidou Ioannis A. Vrotsos |
| author_facet | Andreas S. Gkogkos Ioannis K. Karoussis Ioannis D. Prevezanos Kleopatra E. Marcopoulou Kyriaki Kyriakidou Ioannis A. Vrotsos |
| author_sort | Andreas S. Gkogkos |
| collection | DOAJ |
| description | Aim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors’ secretion (EGF, bFGF, and VEGF). Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the following energy densities: 2.6 J/cm2, 5.3 J/cm2, 7.9 J/cm2, and 15.8 J/cm2. Controls were not irradiated. Cultures were examined for cell proliferation and growth factors’ secretion after 24, 48, and 72 hours. All experimental procedures were performed in duplicate. Data were analyzed by Student’s t-test (p<0.05). Results. All laser-irradiation doses applied promoted a higher cell proliferation at 48 hours in a dose-response relationship compared to controls. This difference reached statistical significance for the cultures receiving 15.8 J/cm2 (p=0.03). Regarding EGF, all laser irradiation doses applied promoted a higher secretion at 48 hours in a reverse dose-response pattern compared to controls. This difference reached statistical significance for the cultures receiving 2.6 J/cm2 (p=0.04). EGF levels at the other time points, bFGF, and VEGF showed a random variation between the groups. Conclusion. Within the limits of this study, LLLT (Nd:YAG) may induce gingival fibroblasts’ proliferation and upregulate the secretion of EGF. Further studies are needed to confirm these results. |
| format | Article |
| id | doaj-art-ae9d6b28b9c5446cb5543ee076231995 |
| institution | OA Journals |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-ae9d6b28b9c5446cb5543ee0762319952025-08-20T02:23:08ZengWileyInternational Journal of Dentistry1687-87281687-87362015-01-01201510.1155/2015/258941258941Effect of Nd:YAG Low Level Laser Therapy on Human Gingival FibroblastsAndreas S. Gkogkos0Ioannis K. Karoussis1Ioannis D. Prevezanos2Kleopatra E. Marcopoulou3Kyriaki Kyriakidou4Ioannis A. Vrotsos5Department of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceDepartment of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceDepartment of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceDepartment of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceDepartment of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceDepartment of Periodontology, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 115 27 Athens, GreeceAim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors’ secretion (EGF, bFGF, and VEGF). Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the following energy densities: 2.6 J/cm2, 5.3 J/cm2, 7.9 J/cm2, and 15.8 J/cm2. Controls were not irradiated. Cultures were examined for cell proliferation and growth factors’ secretion after 24, 48, and 72 hours. All experimental procedures were performed in duplicate. Data were analyzed by Student’s t-test (p<0.05). Results. All laser-irradiation doses applied promoted a higher cell proliferation at 48 hours in a dose-response relationship compared to controls. This difference reached statistical significance for the cultures receiving 15.8 J/cm2 (p=0.03). Regarding EGF, all laser irradiation doses applied promoted a higher secretion at 48 hours in a reverse dose-response pattern compared to controls. This difference reached statistical significance for the cultures receiving 2.6 J/cm2 (p=0.04). EGF levels at the other time points, bFGF, and VEGF showed a random variation between the groups. Conclusion. Within the limits of this study, LLLT (Nd:YAG) may induce gingival fibroblasts’ proliferation and upregulate the secretion of EGF. Further studies are needed to confirm these results.http://dx.doi.org/10.1155/2015/258941 |
| spellingShingle | Andreas S. Gkogkos Ioannis K. Karoussis Ioannis D. Prevezanos Kleopatra E. Marcopoulou Kyriaki Kyriakidou Ioannis A. Vrotsos Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts International Journal of Dentistry |
| title | Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts |
| title_full | Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts |
| title_fullStr | Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts |
| title_full_unstemmed | Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts |
| title_short | Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts |
| title_sort | effect of nd yag low level laser therapy on human gingival fibroblasts |
| url | http://dx.doi.org/10.1155/2015/258941 |
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