Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry
Platelet counting is a fundamental clinical test for diagnosing haemorrhagic diseases, coagulation abnormalities, and certain autoimmune disorders, and it also serves as a critical basis for decisions regarding platelet transfusion. Common automated methods for platelet counting include the internat...
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De Gruyter
2025-02-01
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| Series: | Journal of Laboratory Medicine |
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| Online Access: | https://doi.org/10.1515/labmed-2024-0135 |
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| author | Chen Yong Hu Yonggang Hu Hai |
| author_facet | Chen Yong Hu Yonggang Hu Hai |
| author_sort | Chen Yong |
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| description | Platelet counting is a fundamental clinical test for diagnosing haemorrhagic diseases, coagulation abnormalities, and certain autoimmune disorders, and it also serves as a critical basis for decisions regarding platelet transfusion. Common automated methods for platelet counting include the international harmonization protocol (IHP) based on flow cytometry, CD61 immunoplatelet count (CD61-imm), impedance platelet count (PLT-I), hybrid platelet count (PLT-H), optical platelet count (PLT-O), and fluorescence platelet count (PLT-F). The IHP, based on flow cytometry, is recommended as the reference measurement procedure (RMP) by the Ministry of Health of the People’s Republic of China, the International Council for Standardization in Hematology (ICSH), and the International Society of Laboratory Hematology (ISLH) due to its superior precision and accuracy. Despite the significant improvements in efficiency and standardization brought about by automation, traditional blood smear microscopic examination (PLT-M) remains indispensable in specific scenarios, such as low platelet counts or abnormal platelet morphology, to ensure the accuracy and reliability of platelet counting results from automated methods. |
| format | Article |
| id | doaj-art-ae9a66873c2942ae83d0f57db7956701 |
| institution | Kabale University |
| issn | 2567-9430 2567-9449 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | De Gruyter |
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| series | Journal of Laboratory Medicine |
| spelling | doaj-art-ae9a66873c2942ae83d0f57db79567012025-02-10T13:24:33ZengDe GruyterJournal of Laboratory Medicine2567-94302567-94492025-02-0149121310.1515/labmed-2024-0135Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometryChen Yong0Hu Yonggang1Hu Hai2Department of Clinical Laboratory, People’s Hospital of Naxi District, Luzhou City, Sichuan Province, ChinaDepartment of Clinical Laboratory, People’s Hospital of Naxi District, Luzhou City, Sichuan Province, ChinaDepartment of Clinical Laboratory, People’s Hospital of Naxi District, Luzhou City, Sichuan Province, ChinaPlatelet counting is a fundamental clinical test for diagnosing haemorrhagic diseases, coagulation abnormalities, and certain autoimmune disorders, and it also serves as a critical basis for decisions regarding platelet transfusion. Common automated methods for platelet counting include the international harmonization protocol (IHP) based on flow cytometry, CD61 immunoplatelet count (CD61-imm), impedance platelet count (PLT-I), hybrid platelet count (PLT-H), optical platelet count (PLT-O), and fluorescence platelet count (PLT-F). The IHP, based on flow cytometry, is recommended as the reference measurement procedure (RMP) by the Ministry of Health of the People’s Republic of China, the International Council for Standardization in Hematology (ICSH), and the International Society of Laboratory Hematology (ISLH) due to its superior precision and accuracy. Despite the significant improvements in efficiency and standardization brought about by automation, traditional blood smear microscopic examination (PLT-M) remains indispensable in specific scenarios, such as low platelet counts or abnormal platelet morphology, to ensure the accuracy and reliability of platelet counting results from automated methods.https://doi.org/10.1515/labmed-2024-0135platelet countthe international harmonization protocolcd61 immunoplatelet countimpedance platelet countautomated methodsmicroscopic methods |
| spellingShingle | Chen Yong Hu Yonggang Hu Hai Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry Journal of Laboratory Medicine platelet count the international harmonization protocol cd61 immunoplatelet count impedance platelet count automated methods microscopic methods |
| title | Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry |
| title_full | Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry |
| title_fullStr | Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry |
| title_full_unstemmed | Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry |
| title_short | Advances and challenges in platelet counting: evolving from traditional microscopy to modern flow cytometry |
| title_sort | advances and challenges in platelet counting evolving from traditional microscopy to modern flow cytometry |
| topic | platelet count the international harmonization protocol cd61 immunoplatelet count impedance platelet count automated methods microscopic methods |
| url | https://doi.org/10.1515/labmed-2024-0135 |
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