Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice
<b>Background/Objectives</b>: Hallucinogenic substances such as psilocybin, psilocin, ergometrine, ergotamine, and lysergic acid diethylamide (LSD) have been demonstrated to enhance the force of contraction (FOC), in part due to the phosphorylation of phospholamban in human atrial prepar...
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| author | Pauline Braekow Joachim Neumann Uwe Kirchhefer Ulrich Gergs |
| author_facet | Pauline Braekow Joachim Neumann Uwe Kirchhefer Ulrich Gergs |
| author_sort | Pauline Braekow |
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| description | <b>Background/Objectives</b>: Hallucinogenic substances such as psilocybin, psilocin, ergometrine, ergotamine, and lysergic acid diethylamide (LSD) have been demonstrated to enhance the force of contraction (FOC), in part due to the phosphorylation of phospholamban in human atrial preparations via 5-HT<sub>4</sub> serotonin receptors and/or H<sub>2</sub> histamine receptors. However, whether psilocybin or psilocin acts at isolated mammalian ventricular preparations and whether they increase protein phosphorylation in the mammalian ventricle remains to be elucidated. <b>Methods</b>: To this end, the FOC and phospholamban phosphorylation in isolated perfused hearts from transgenic mice with cardiomyocyte-specific overexpression of either human 5-HT<sub>4</sub> receptors (5-HT<sub>4</sub>-TG) or human H<sub>2</sub> receptors (H<sub>2</sub>-TG) and their wild-type littermates (WT) were examined. Furthermore, the ergot alkaloids ergometrine, ergotamine, and LSD were used as references. <b>Results</b>: Psilocybin and psilocin enhanced the FOC to 137% and to 152%, respectively, and elevated the phospholamban phosphorylation in isolated perfused hearts from 5-HT<sub>4</sub>-TG. In H<sub>2</sub>-TG hearts, psilocybin and psilocin increased the FOC to a much lesser extent but had no effect on the phospholamban phosphorylation. In contrast, LSD increased the FOC and phosphorylation state of phospholamban in isolated hearts of both 5-HT<sub>4</sub>-TG and H<sub>2</sub>-TG. On the other hand, ergometrine and ergotamine increased the FOC only in H<sub>2</sub>-TG. Ergometrine increased the phosphorylation state of phospholamban in perfused hearts from H<sub>2</sub>-TG, but not from 5-HT<sub>4</sub>-TG. Ergotamine failed to increase the phospholamban phosphorylation in both H<sub>2</sub>-TG and 5-HT<sub>4</sub>-TG. Psilocybin, psilocin, ergotamine, ergometrine, and LSD were unable to increase FOC and phospholamban phosphorylation in perfused hearts from WT. <b>Conclusions</b>: The increase in the phosphorylation state of phospholamban could provide a partial explanation for the positive inotropic effects and the relaxant effects of not only psilocybin and psilocin but also ergometrine and LSD in the isolated hearts of the animals used in this study. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-ae8bfe4541c04b68add30929f7210a8b2025-08-20T03:56:49ZengMDPI AGPharmaceuticals1424-82472025-07-01187100910.3390/ph18071009Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic MicePauline Braekow0Joachim Neumann1Uwe Kirchhefer2Ulrich Gergs3Institute for Pharmacology and Toxicology, Medical Faculty, Martin Luther University Halle-Wittenberg, 06097 Halle, GermanyInstitute for Pharmacology and Toxicology, Medical Faculty, Martin Luther University Halle-Wittenberg, 06097 Halle, GermanyInstitute of Pharmacology and Toxicology, University of Münster, 48149 Münster, GermanyInstitute for Pharmacology and Toxicology, Medical Faculty, Martin Luther University Halle-Wittenberg, 06097 Halle, Germany<b>Background/Objectives</b>: Hallucinogenic substances such as psilocybin, psilocin, ergometrine, ergotamine, and lysergic acid diethylamide (LSD) have been demonstrated to enhance the force of contraction (FOC), in part due to the phosphorylation of phospholamban in human atrial preparations via 5-HT<sub>4</sub> serotonin receptors and/or H<sub>2</sub> histamine receptors. However, whether psilocybin or psilocin acts at isolated mammalian ventricular preparations and whether they increase protein phosphorylation in the mammalian ventricle remains to be elucidated. <b>Methods</b>: To this end, the FOC and phospholamban phosphorylation in isolated perfused hearts from transgenic mice with cardiomyocyte-specific overexpression of either human 5-HT<sub>4</sub> receptors (5-HT<sub>4</sub>-TG) or human H<sub>2</sub> receptors (H<sub>2</sub>-TG) and their wild-type littermates (WT) were examined. Furthermore, the ergot alkaloids ergometrine, ergotamine, and LSD were used as references. <b>Results</b>: Psilocybin and psilocin enhanced the FOC to 137% and to 152%, respectively, and elevated the phospholamban phosphorylation in isolated perfused hearts from 5-HT<sub>4</sub>-TG. In H<sub>2</sub>-TG hearts, psilocybin and psilocin increased the FOC to a much lesser extent but had no effect on the phospholamban phosphorylation. In contrast, LSD increased the FOC and phosphorylation state of phospholamban in isolated hearts of both 5-HT<sub>4</sub>-TG and H<sub>2</sub>-TG. On the other hand, ergometrine and ergotamine increased the FOC only in H<sub>2</sub>-TG. Ergometrine increased the phosphorylation state of phospholamban in perfused hearts from H<sub>2</sub>-TG, but not from 5-HT<sub>4</sub>-TG. Ergotamine failed to increase the phospholamban phosphorylation in both H<sub>2</sub>-TG and 5-HT<sub>4</sub>-TG. Psilocybin, psilocin, ergotamine, ergometrine, and LSD were unable to increase FOC and phospholamban phosphorylation in perfused hearts from WT. <b>Conclusions</b>: The increase in the phosphorylation state of phospholamban could provide a partial explanation for the positive inotropic effects and the relaxant effects of not only psilocybin and psilocin but also ergometrine and LSD in the isolated hearts of the animals used in this study.https://www.mdpi.com/1424-8247/18/7/1009psilocybinpsilocinergometrineergotamineLSDLangendorff heart |
| spellingShingle | Pauline Braekow Joachim Neumann Uwe Kirchhefer Ulrich Gergs Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice Pharmaceuticals psilocybin psilocin ergometrine ergotamine LSD Langendorff heart |
| title | Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice |
| title_full | Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice |
| title_fullStr | Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice |
| title_full_unstemmed | Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice |
| title_short | Effects of Psilocin and Psilocybin on Human 5-HT<sub>4</sub> Serotonin and H<sub>2</sub> Histamine Receptors in Perfused Hearts of Transgenic Mice |
| title_sort | effects of psilocin and psilocybin on human 5 ht sub 4 sub serotonin and h sub 2 sub histamine receptors in perfused hearts of transgenic mice |
| topic | psilocybin psilocin ergometrine ergotamine LSD Langendorff heart |
| url | https://www.mdpi.com/1424-8247/18/7/1009 |
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