Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats
Brahma-related gene 1 (Brg1) is a key gene in inducing the expression of important endogenous antioxidant enzymes, including heme oxygenase-1 (HO-1) which is central to cardioprotection, while cardiac HO-1 expression is reduced in diabetes. It is unknown whether or not cardiac Brg1 expression is red...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2013/716219 |
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| author | Jinjin Xu Shaoqing Lei Yanan Liu Xia Gao Michael G. Irwin Zhong-yuan Xia Ziqing Hei Xiaoliang Gan Tingting Wang Zhengyuan Xia |
| author_facet | Jinjin Xu Shaoqing Lei Yanan Liu Xia Gao Michael G. Irwin Zhong-yuan Xia Ziqing Hei Xiaoliang Gan Tingting Wang Zhengyuan Xia |
| author_sort | Jinjin Xu |
| collection | DOAJ |
| description | Brahma-related gene 1 (Brg1) is a key gene in inducing the expression of important endogenous antioxidant enzymes, including heme oxygenase-1 (HO-1) which is central to cardioprotection, while cardiac HO-1 expression is reduced in diabetes. It is unknown whether or not cardiac Brg1 expression is reduced in diabetes. We hypothesize that cardiac Brg1 expression is reduced in diabetes which can be restored by antioxidant treatment with N-acetylcysteine (NAC). Control (C) and streptozotocin-induced diabetic (D) rats were treated with NAC in drinking water or placebo for 4 weeks. Plasma and cardiac free15-F2t-isoprostane in diabetic rats were increased, accompanied with increased plasma levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6), while cardiac Brg1, p-STAT3 and HO-1 protein expression levels were significantly decreased. Left ventricle weight/body weight ratio was higher, while the peak velocities of early (E) and late (A) flow ratio was lower in diabetic than in C rats. NAC normalized tissue and plasma levels of 15-F2t-isoprostane, significantly increased cardiac Brg1, HO-1 and p-STAT3 protein expression levels and reduced TNF-alpha and IL-6, resulting in improved cardiac function. In conclusion, myocardial Brg1 is reduced in diabetes and enhancement of cardiac Brg1 expression may represent a novel mechanism whereby NAC confers cardioprotection. |
| format | Article |
| id | doaj-art-ae8804e2e32d4fa68cd76dcd9d7448ac |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-ae8804e2e32d4fa68cd76dcd9d7448ac2025-08-20T02:23:08ZengWileyJournal of Diabetes Research2314-67452314-67532013-01-01201310.1155/2013/716219716219Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic RatsJinjin Xu0Shaoqing Lei1Yanan Liu2Xia Gao3Michael G. Irwin4Zhong-yuan Xia5Ziqing Hei6Xiaoliang Gan7Tingting Wang8Zhengyuan Xia9Department of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Anesthesiology, 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Anesthesiology, 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongDepartment of Anaesthesiology, The University of Hong Kong, HKSAR, Hong KongBrahma-related gene 1 (Brg1) is a key gene in inducing the expression of important endogenous antioxidant enzymes, including heme oxygenase-1 (HO-1) which is central to cardioprotection, while cardiac HO-1 expression is reduced in diabetes. It is unknown whether or not cardiac Brg1 expression is reduced in diabetes. We hypothesize that cardiac Brg1 expression is reduced in diabetes which can be restored by antioxidant treatment with N-acetylcysteine (NAC). Control (C) and streptozotocin-induced diabetic (D) rats were treated with NAC in drinking water or placebo for 4 weeks. Plasma and cardiac free15-F2t-isoprostane in diabetic rats were increased, accompanied with increased plasma levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6), while cardiac Brg1, p-STAT3 and HO-1 protein expression levels were significantly decreased. Left ventricle weight/body weight ratio was higher, while the peak velocities of early (E) and late (A) flow ratio was lower in diabetic than in C rats. NAC normalized tissue and plasma levels of 15-F2t-isoprostane, significantly increased cardiac Brg1, HO-1 and p-STAT3 protein expression levels and reduced TNF-alpha and IL-6, resulting in improved cardiac function. In conclusion, myocardial Brg1 is reduced in diabetes and enhancement of cardiac Brg1 expression may represent a novel mechanism whereby NAC confers cardioprotection.http://dx.doi.org/10.1155/2013/716219 |
| spellingShingle | Jinjin Xu Shaoqing Lei Yanan Liu Xia Gao Michael G. Irwin Zhong-yuan Xia Ziqing Hei Xiaoliang Gan Tingting Wang Zhengyuan Xia Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats Journal of Diabetes Research |
| title | Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats |
| title_full | Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats |
| title_fullStr | Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats |
| title_full_unstemmed | Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats |
| title_short | Antioxidant N-Acetylcysteine Attenuates the Reduction of Brg1 Protein Expression in the Myocardium of Type 1 Diabetic Rats |
| title_sort | antioxidant n acetylcysteine attenuates the reduction of brg1 protein expression in the myocardium of type 1 diabetic rats |
| url | http://dx.doi.org/10.1155/2013/716219 |
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