Gene-specific features of tick-borne borreliosis in Russia
Aim. To study the gene-specific composition of Borrelia spp. in ixodid ticks inhabiting the foci of ixodid tick-borne borreliosis (ITBB) in the south of Western Siberia.Materials and Methods. The gene-specific composition of Borrelia spp. in ixodid ticks was determined by inoculation on a BSK-H nutr...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State Medical University
2021-09-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/441 |
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| author | S. A. Rudakova N. V. Rudakov S. V. Shtrek O. E. Teslova N. E. Kaneshova |
| author_facet | S. A. Rudakova N. V. Rudakov S. V. Shtrek O. E. Teslova N. E. Kaneshova |
| author_sort | S. A. Rudakova |
| collection | DOAJ |
| description | Aim. To study the gene-specific composition of Borrelia spp. in ixodid ticks inhabiting the foci of ixodid tick-borne borreliosis (ITBB) in the south of Western Siberia.Materials and Methods. The gene-specific composition of Borrelia spp. in ixodid ticks was determined by inoculation on a BSK-H nutrient medium, real-time polymerase chain reaction, and sequencing (1148 specimens of ixodid ticks collected from vegetation and 2183 specimens withdrawn from humans).Results. Infection of ticks with borrelia ranged from 22.4% in the Altai Republic to 56.9% in the Novosibirsk Region. There were no significant differences in the levels of Borrelia infection between two major ixodides, I. persulcatus and I. pavlovskiy (average infection levels 40.0% and 38.8%, respectively). At least five gene species of pathogenic Borrelia (B. garinii, B. afzelii, B. bavariensis, B. miyamotoi, and B. spielmanii) have been identified. The GenBank database contains 45 nucleotide sequences of the intergenic spacer rrf (5S)-rrl (23S). Prevalence of gene species B. garinii and B. afzelii in different ticks (I. persulcatus and I. pavlovskiy) did not differ significantly, yet B. garinii was found to be more frequent than B. afzelii. The frequency of infection of I. persulcatus ticks with B. miyamotoi was significantly (3.5-fold) as compared to those of B. garinii and B. afzelii. In D. reticulatus ticks, the DNA of B. spielmanii and B. miyamotoi was detected.Conclusion. It is necessary to continue studies to assess the role of the meadow ticks D. reticulatus in the circulation of different Borrelia spp. in various natural foci within the Russian Federation. |
| format | Article |
| id | doaj-art-ae6ab2affb514237a286cd6f88f12cca |
| institution | Kabale University |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2021-09-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-ae6ab2affb514237a286cd6f88f12cca2025-08-20T03:42:38ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412021-09-0163949910.23946/2500-0764-2021-6-3-94-99280Gene-specific features of tick-borne borreliosis in RussiaS. A. Rudakova0N. V. Rudakov1S. V. Shtrek2O. E. Teslova3N. E. Kaneshova4Omsk Research Institute of Natural Focal InfectionsOmsk Research Institute of Natural Focal Infections; Omsk State Medical UniversityOmsk Research Institute of Natural Focal Infections; Omsk State Medical UniversityOmsk Research Institute of Natural Focal Infections; Omsk State Medical UniversityOmsk Research Institute of Natural Focal Infections; Omsk State Medical UniversityAim. To study the gene-specific composition of Borrelia spp. in ixodid ticks inhabiting the foci of ixodid tick-borne borreliosis (ITBB) in the south of Western Siberia.Materials and Methods. The gene-specific composition of Borrelia spp. in ixodid ticks was determined by inoculation on a BSK-H nutrient medium, real-time polymerase chain reaction, and sequencing (1148 specimens of ixodid ticks collected from vegetation and 2183 specimens withdrawn from humans).Results. Infection of ticks with borrelia ranged from 22.4% in the Altai Republic to 56.9% in the Novosibirsk Region. There were no significant differences in the levels of Borrelia infection between two major ixodides, I. persulcatus and I. pavlovskiy (average infection levels 40.0% and 38.8%, respectively). At least five gene species of pathogenic Borrelia (B. garinii, B. afzelii, B. bavariensis, B. miyamotoi, and B. spielmanii) have been identified. The GenBank database contains 45 nucleotide sequences of the intergenic spacer rrf (5S)-rrl (23S). Prevalence of gene species B. garinii and B. afzelii in different ticks (I. persulcatus and I. pavlovskiy) did not differ significantly, yet B. garinii was found to be more frequent than B. afzelii. The frequency of infection of I. persulcatus ticks with B. miyamotoi was significantly (3.5-fold) as compared to those of B. garinii and B. afzelii. In D. reticulatus ticks, the DNA of B. spielmanii and B. miyamotoi was detected.Conclusion. It is necessary to continue studies to assess the role of the meadow ticks D. reticulatus in the circulation of different Borrelia spp. in various natural foci within the Russian Federation.https://fcm.kemsmu.ru/jour/article/view/441ixodid ticksborreliatick-borne borreliosis |
| spellingShingle | S. A. Rudakova N. V. Rudakov S. V. Shtrek O. E. Teslova N. E. Kaneshova Gene-specific features of tick-borne borreliosis in Russia Фундаментальная и клиническая медицина ixodid ticks borrelia tick-borne borreliosis |
| title | Gene-specific features of tick-borne borreliosis in Russia |
| title_full | Gene-specific features of tick-borne borreliosis in Russia |
| title_fullStr | Gene-specific features of tick-borne borreliosis in Russia |
| title_full_unstemmed | Gene-specific features of tick-borne borreliosis in Russia |
| title_short | Gene-specific features of tick-borne borreliosis in Russia |
| title_sort | gene specific features of tick borne borreliosis in russia |
| topic | ixodid ticks borrelia tick-borne borreliosis |
| url | https://fcm.kemsmu.ru/jour/article/view/441 |
| work_keys_str_mv | AT sarudakova genespecificfeaturesoftickborneborreliosisinrussia AT nvrudakov genespecificfeaturesoftickborneborreliosisinrussia AT svshtrek genespecificfeaturesoftickborneborreliosisinrussia AT oeteslova genespecificfeaturesoftickborneborreliosisinrussia AT nekaneshova genespecificfeaturesoftickborneborreliosisinrussia |