Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4
Simulated body fluid with and without bovine serum albumin (1 mg/ml and 10 mg/ml) was used to study the impact of proteins on bioactive glass S53P4 reactions in vitro. The glass particles were exposed to dynamic and static solutions for up to 6 days. Also, a 3-day simulated body fluid pretreatment w...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Open Ceramics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666539525000148 |
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| author | Minna Siekkinen Adrian Stiller Xiaoju Wang Leena Hupa |
| author_facet | Minna Siekkinen Adrian Stiller Xiaoju Wang Leena Hupa |
| author_sort | Minna Siekkinen |
| collection | DOAJ |
| description | Simulated body fluid with and without bovine serum albumin (1 mg/ml and 10 mg/ml) was used to study the impact of proteins on bioactive glass S53P4 reactions in vitro. The glass particles were exposed to dynamic and static solutions for up to 6 days. Also, a 3-day simulated body fluid pretreatment was done before an additional 3-day exposure to protein solutions. The solution with a higher protein concentration hindered calcium phosphate precipitation on samples in static conditions, whereas the protein did not affect calcium phosphate precipitation in dynamic conditions. Protein adsorbed slightly more on static samples. The protein concentrations suggested immediate adsorption in the dynamic experiments, implying that calcium phosphate is not needed for adsorption. The results indicated that in low protein concentration solutions in vitro, the protein might bind to calcium ions, thus affecting protein adsorption and calcium phosphate precipitation. |
| format | Article |
| id | doaj-art-ae682fb464584ccaa14842ebc6f12ce9 |
| institution | Kabale University |
| issn | 2666-5395 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Open Ceramics |
| spelling | doaj-art-ae682fb464584ccaa14842ebc6f12ce92025-02-12T05:32:52ZengElsevierOpen Ceramics2666-53952025-03-0121100747Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4Minna Siekkinen0Adrian Stiller1Xiaoju Wang2Leena Hupa3Johan Gadolin Process Chemistry Centre, High-Temperature Processes and Materials, Åbo Akademi University, Henrikinkatu 2, 20500 Turku, Finland; Corresponding author.Johan Gadolin Process Chemistry Centre, High-Temperature Processes and Materials, Åbo Akademi University, Henrikinkatu 2, 20500 Turku, FinlandPharmaceutical Sciences Laboratory, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland; Laboratory of Natural Materials Technology, Åbo Akademi University, Henrikinkatu 2, 20500 Turku, FinlandJohan Gadolin Process Chemistry Centre, High-Temperature Processes and Materials, Åbo Akademi University, Henrikinkatu 2, 20500 Turku, FinlandSimulated body fluid with and without bovine serum albumin (1 mg/ml and 10 mg/ml) was used to study the impact of proteins on bioactive glass S53P4 reactions in vitro. The glass particles were exposed to dynamic and static solutions for up to 6 days. Also, a 3-day simulated body fluid pretreatment was done before an additional 3-day exposure to protein solutions. The solution with a higher protein concentration hindered calcium phosphate precipitation on samples in static conditions, whereas the protein did not affect calcium phosphate precipitation in dynamic conditions. Protein adsorbed slightly more on static samples. The protein concentrations suggested immediate adsorption in the dynamic experiments, implying that calcium phosphate is not needed for adsorption. The results indicated that in low protein concentration solutions in vitro, the protein might bind to calcium ions, thus affecting protein adsorption and calcium phosphate precipitation.http://www.sciencedirect.com/science/article/pii/S2666539525000148Bioactive glassIn vitroBovine serum albuminSimulated body fluidSurface characterization |
| spellingShingle | Minna Siekkinen Adrian Stiller Xiaoju Wang Leena Hupa Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 Open Ceramics Bioactive glass In vitro Bovine serum albumin Simulated body fluid Surface characterization |
| title | Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 |
| title_full | Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 |
| title_fullStr | Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 |
| title_full_unstemmed | Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 |
| title_short | Impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass S53P4 |
| title_sort | impact of bovine serum albumin in simulated body fluid on the dynamic dissolution behavior of bioactive glass s53p4 |
| topic | Bioactive glass In vitro Bovine serum albumin Simulated body fluid Surface characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2666539525000148 |
| work_keys_str_mv | AT minnasiekkinen impactofbovineserumalbumininsimulatedbodyfluidonthedynamicdissolutionbehaviorofbioactiveglasss53p4 AT adrianstiller impactofbovineserumalbumininsimulatedbodyfluidonthedynamicdissolutionbehaviorofbioactiveglasss53p4 AT xiaojuwang impactofbovineserumalbumininsimulatedbodyfluidonthedynamicdissolutionbehaviorofbioactiveglasss53p4 AT leenahupa impactofbovineserumalbumininsimulatedbodyfluidonthedynamicdissolutionbehaviorofbioactiveglasss53p4 |