Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya
Background: Salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. The presence of Salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard...
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The Journal of Infection in Developing Countries
2010-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/446 |
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| author | Gideon M. Kikuvi Jackson N. Ombui Eric S. Mitema |
| author_facet | Gideon M. Kikuvi Jackson N. Ombui Eric S. Mitema |
| author_sort | Gideon M. Kikuvi |
| collection | DOAJ |
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Background: Salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. The presence of Salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard.
Methodology: Samples were collected from randomly selected pigs at the Ndumbuini abattoir in Nairobi. Isolates were confirmed to be Salmonella by biochemical tests and characterised by serotyping, phage typing and plasmid analysis. Minimum inhibitory concentrations (MICs) of eight antimicrobials were determined and the resistant isolates were screened for resistance genes by PCR.
Results: Sixteen (13.8%) of 116 samples were positive for Salmonella. Three Salmonella enterica subsp. enterica serovars, namely Saintpaul, Braenderup, and Heidelberg were identified, S. Saintpaul being predominant. Antimicrobial resistance was found in 35.7% of the isolates. The S. Heidelberg isolates were susceptible to all the antimicrobials tested. Multidrug resistance was found in 7.1% of the Salmonella isolates. Plasmids were only detected in S. Heidelberg. Ampicillin resistance was based on expression of a blaTEM gene, while chloramphenicol, streptomycin, and tetracycline resistances were encoded by the genes catA1, strA, and tet(A), respectively.
Conclusions: Pigs may serve as reservoirs of antimicrobial resistant Salmonella and slaughterhouse cross-contamination of pork may be a food safety risk.
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| format | Article |
| id | doaj-art-ae63bb2cbefd420da644d13629257a26 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-03-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ae63bb2cbefd420da644d13629257a262025-08-20T02:27:15ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-03-0140410.3855/jidc.446Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in KenyaGideon M. Kikuvi0Jackson N. Ombui1Eric S. Mitema2Jomo Kenyatta University of Agriculture and Technology, NairobiUniversity of NairobiUniversity of Nairobi Background: Salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. The presence of Salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard. Methodology: Samples were collected from randomly selected pigs at the Ndumbuini abattoir in Nairobi. Isolates were confirmed to be Salmonella by biochemical tests and characterised by serotyping, phage typing and plasmid analysis. Minimum inhibitory concentrations (MICs) of eight antimicrobials were determined and the resistant isolates were screened for resistance genes by PCR. Results: Sixteen (13.8%) of 116 samples were positive for Salmonella. Three Salmonella enterica subsp. enterica serovars, namely Saintpaul, Braenderup, and Heidelberg were identified, S. Saintpaul being predominant. Antimicrobial resistance was found in 35.7% of the isolates. The S. Heidelberg isolates were susceptible to all the antimicrobials tested. Multidrug resistance was found in 7.1% of the Salmonella isolates. Plasmids were only detected in S. Heidelberg. Ampicillin resistance was based on expression of a blaTEM gene, while chloramphenicol, streptomycin, and tetracycline resistances were encoded by the genes catA1, strA, and tet(A), respectively. Conclusions: Pigs may serve as reservoirs of antimicrobial resistant Salmonella and slaughterhouse cross-contamination of pork may be a food safety risk. https://jidc.org/index.php/journal/article/view/446SerotypesAntimicrobial ResistanceSalmonellaPigsKenya |
| spellingShingle | Gideon M. Kikuvi Jackson N. Ombui Eric S. Mitema Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya Journal of Infection in Developing Countries Serotypes Antimicrobial Resistance Salmonella Pigs Kenya |
| title | Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya |
| title_full | Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya |
| title_fullStr | Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya |
| title_full_unstemmed | Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya |
| title_short | Serotypes and antimicrobial resistance profiles of Salmonella isolates from pigs at slaughter in Kenya |
| title_sort | serotypes and antimicrobial resistance profiles of salmonella isolates from pigs at slaughter in kenya |
| topic | Serotypes Antimicrobial Resistance Salmonella Pigs Kenya |
| url | https://jidc.org/index.php/journal/article/view/446 |
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