Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga
ABSTRACT Saccharina japonica, one of the most widely cultivated brown algae species, is considered a promising biorefinery feedstock due to its high carbohydrate content. Dilute acid hydrolysis can be performed to recover sugars from S. japonica; however, the impact of sugar derivatives (potential i...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | GCB Bioenergy |
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| Online Access: | https://doi.org/10.1111/gcbb.70038 |
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| author | Jihyun Bae Jeongho Lee Hyeok Ki Kwon Giwon Lee Taek Lee Hyun Gyu Lim Sang Woo Seo Gyoo Yeol Jung Hah Young Yoo Chulhwan Park |
| author_facet | Jihyun Bae Jeongho Lee Hyeok Ki Kwon Giwon Lee Taek Lee Hyun Gyu Lim Sang Woo Seo Gyoo Yeol Jung Hah Young Yoo Chulhwan Park |
| author_sort | Jihyun Bae |
| collection | DOAJ |
| description | ABSTRACT Saccharina japonica, one of the most widely cultivated brown algae species, is considered a promising biorefinery feedstock due to its high carbohydrate content. Dilute acid hydrolysis can be performed to recover sugars from S. japonica; however, the impact of sugar derivatives (potential inhibitors) generated during the hydrolysis process on lactic acid production remains unexplored. In this study, the inhibitory effects of sugar derivatives on the fermentation performance of Lacticaseibacillus rhamnosus were systematically examined to enhance the bioconversion efficiency of S. japonica. Firstly, the sugar derivatives present in S. japonica hydrolysate were identified, revealing the presence of acetic acid, formic acid, and furfural. Subsequently, their inhibitory effects on lactic acid production were assessed, demonstrating significant inhibition (p < 0.05) at the following concentrations: > 2 g/L acetic acid, > 0.5 g/L formic acid, and > 1 g/L furfural. Based on the information, 5% H2SO4 was determined to be the optimal solvent for S. japonica hydrolysis, enabling the production of hydrolysate with high fermentable sugar content and minimal sugar derivatives: 23.23 g/L mannitol, 0.86 g/L glucose, 0.21 g/L acetic acid, 0.14 g/L formic acid, and no detectable furfural. The resulting S. japonica hydrolysate contained sugar derivatives at non‐inhibitory levels, allowing for direct application to fermentation without detoxification. As a result, lactic acid production and yield were determined to be 18.26 g/L and 92.3%, respectively, comparable to the control group (17.32 g/L and 87.6%). This study addresses a critical knowledge gap in the bioconversion of macroalgae to lactic acid by elucidating the effects of sugar derivatives on fermentation performance. |
| format | Article |
| id | doaj-art-ae61ecd71b53426088de76b4e6c8f5e7 |
| institution | OA Journals |
| issn | 1757-1693 1757-1707 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj-art-ae61ecd71b53426088de76b4e6c8f5e72025-08-20T02:28:42ZengWileyGCB Bioenergy1757-16931757-17072025-05-01175n/an/a10.1111/gcbb.70038Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown MacroalgaJihyun Bae0Jeongho Lee1Hyeok Ki Kwon2Giwon Lee3Taek Lee4Hyun Gyu Lim5Sang Woo Seo6Gyoo Yeol Jung7Hah Young Yoo8Chulhwan Park9Department of Chemical Engineering Kwangwoon University Seoul Republic of KoreaDepartment of Biotechnology Sangmyung University Seoul Republic of KoreaDepartment of Chemical Engineering Kwangwoon University Seoul Republic of KoreaDepartment of Chemical Engineering Kwangwoon University Seoul Republic of KoreaDepartment of Chemical Engineering Kwangwoon University Seoul Republic of KoreaDepartment of Biological Sciences and Bioengineering Inha University Incheon Republic of KoreaSchool of Chemical and Biological Engineering, Institute of Chemical Processes, Bio‐MAX Institute, Institute of Bio Engineering Seoul National University Seoul Republic of KoreaDepartment of Chemical Engineering Pohang University of Science and Technology Pohang Republic of KoreaDepartment of Biotechnology Sangmyung University Seoul Republic of KoreaDepartment of Chemical Engineering Kwangwoon University Seoul Republic of KoreaABSTRACT Saccharina japonica, one of the most widely cultivated brown algae species, is considered a promising biorefinery feedstock due to its high carbohydrate content. Dilute acid hydrolysis can be performed to recover sugars from S. japonica; however, the impact of sugar derivatives (potential inhibitors) generated during the hydrolysis process on lactic acid production remains unexplored. In this study, the inhibitory effects of sugar derivatives on the fermentation performance of Lacticaseibacillus rhamnosus were systematically examined to enhance the bioconversion efficiency of S. japonica. Firstly, the sugar derivatives present in S. japonica hydrolysate were identified, revealing the presence of acetic acid, formic acid, and furfural. Subsequently, their inhibitory effects on lactic acid production were assessed, demonstrating significant inhibition (p < 0.05) at the following concentrations: > 2 g/L acetic acid, > 0.5 g/L formic acid, and > 1 g/L furfural. Based on the information, 5% H2SO4 was determined to be the optimal solvent for S. japonica hydrolysis, enabling the production of hydrolysate with high fermentable sugar content and minimal sugar derivatives: 23.23 g/L mannitol, 0.86 g/L glucose, 0.21 g/L acetic acid, 0.14 g/L formic acid, and no detectable furfural. The resulting S. japonica hydrolysate contained sugar derivatives at non‐inhibitory levels, allowing for direct application to fermentation without detoxification. As a result, lactic acid production and yield were determined to be 18.26 g/L and 92.3%, respectively, comparable to the control group (17.32 g/L and 87.6%). This study addresses a critical knowledge gap in the bioconversion of macroalgae to lactic acid by elucidating the effects of sugar derivatives on fermentation performance.https://doi.org/10.1111/gcbb.70038acid hydrolysisalgaebio‐value chaininhibitory effectlactic acidSaccharina japonica |
| spellingShingle | Jihyun Bae Jeongho Lee Hyeok Ki Kwon Giwon Lee Taek Lee Hyun Gyu Lim Sang Woo Seo Gyoo Yeol Jung Hah Young Yoo Chulhwan Park Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga GCB Bioenergy acid hydrolysis algae bio‐value chain inhibitory effect lactic acid Saccharina japonica |
| title | Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga |
| title_full | Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga |
| title_fullStr | Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga |
| title_full_unstemmed | Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga |
| title_short | Strategy for Sustainable Bio‐Value Chain Development: Algal Biomass Utilization Without Inhibitory Effects on Lactic Acid Production via Efficient Diluted Acid Hydrolysis of Brown Macroalga |
| title_sort | strategy for sustainable bio value chain development algal biomass utilization without inhibitory effects on lactic acid production via efficient diluted acid hydrolysis of brown macroalga |
| topic | acid hydrolysis algae bio‐value chain inhibitory effect lactic acid Saccharina japonica |
| url | https://doi.org/10.1111/gcbb.70038 |
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