Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells
Long non-coding RNA (lncRNA) plays important biological regulatory functions at different levels. lncRNA NONRATT021477.2 (lncRNA77.2) was identified as a key gene under cold stress conditions in rats. From the literature, lncRNA77.2 may have important roles during antioxidant, but this conjecture re...
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Kafkas University, Faculty of Veterinary Medicine
2024-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3153 |
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| author | Hong JI Yue HUA Chun-Wei LIU Zi-Yi SHAO Jia-Jun ZHANG Shui-Qing YU |
| author_facet | Hong JI Yue HUA Chun-Wei LIU Zi-Yi SHAO Jia-Jun ZHANG Shui-Qing YU |
| author_sort | Hong JI |
| collection | DOAJ |
| description | Long non-coding RNA (lncRNA) plays important biological regulatory functions at
different levels. lncRNA NONRATT021477.2 (lncRNA77.2) was identified as a key
gene under cold stress conditions in rats. From the literature, lncRNA77.2 may have
important roles during antioxidant, but this conjecture requires investigation. To address
this knowledge gap, we investigated the effects of lncRNA77.2 interference on H2O2-
induced oxidative stress in rat liver cells (Buffalo rat liver, BRL).In the current study,
H2O2 treatment simulated BRL cell oxidative stress. H2O2 treatment led to a significant
increase in oxidative stress levels in BRL cells, whereas the gene expression levels of
antioxidase and lncRNA 77.2 and cell viability were significantly reduced. Additionally,
the expression of Nrf2 and Keap1 proteins decreased significantly compared to the
control group. BRL cells were transfected with antisense oligonucleotides (ASO) or
a negative control ASO (ASO-NC) and then treated with H2O2. The results showed
lncRNA77.2 interference increased oxidative stress levels, reduced gene expression
of antioxidant enzymes, Nrf2 and Keap1 expression levels. The results indicated that
lncRNA77.2 interference aggravated H2O2-induced oxidative stress in BRL cells, which
suggested that lncRNA77.2 is an antioxidant factor that plays an important role in the
regulation of oxidative stress in BRL cells. |
| format | Article |
| id | doaj-art-ae5fc70b937d4c8d8b8aa261da6618a5 |
| institution | OA Journals |
| issn | 1309-2251 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-ae5fc70b937d4c8d8b8aa261da6618a52025-08-20T02:22:09ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-11-0130677978510.9775/kvfd.2024.322473153Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cellsHong JI0Yue HUA1Chun-Wei LIU2Zi-Yi SHAO3Jia-Jun ZHANG4Shui-Qing YU5College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINACollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINACollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINACollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINACollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINACollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, CHINALong non-coding RNA (lncRNA) plays important biological regulatory functions at different levels. lncRNA NONRATT021477.2 (lncRNA77.2) was identified as a key gene under cold stress conditions in rats. From the literature, lncRNA77.2 may have important roles during antioxidant, but this conjecture requires investigation. To address this knowledge gap, we investigated the effects of lncRNA77.2 interference on H2O2- induced oxidative stress in rat liver cells (Buffalo rat liver, BRL).In the current study, H2O2 treatment simulated BRL cell oxidative stress. H2O2 treatment led to a significant increase in oxidative stress levels in BRL cells, whereas the gene expression levels of antioxidase and lncRNA 77.2 and cell viability were significantly reduced. Additionally, the expression of Nrf2 and Keap1 proteins decreased significantly compared to the control group. BRL cells were transfected with antisense oligonucleotides (ASO) or a negative control ASO (ASO-NC) and then treated with H2O2. The results showed lncRNA77.2 interference increased oxidative stress levels, reduced gene expression of antioxidant enzymes, Nrf2 and Keap1 expression levels. The results indicated that lncRNA77.2 interference aggravated H2O2-induced oxidative stress in BRL cells, which suggested that lncRNA77.2 is an antioxidant factor that plays an important role in the regulation of oxidative stress in BRL cells.https://vetdergikafkas.org/pdf.php?id=3153brl cellslncrnaoxidative stress |
| spellingShingle | Hong JI Yue HUA Chun-Wei LIU Zi-Yi SHAO Jia-Jun ZHANG Shui-Qing YU Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells Kafkas Universitesi Veteriner Fakültesi Dergisi brl cells lncrna oxidative stress |
| title | Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells |
| title_full | Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells |
| title_fullStr | Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells |
| title_full_unstemmed | Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells |
| title_short | Lncrna nonratt021477.2 interference aggravates h2o2-induced oxidative stress in brl cells |
| title_sort | lncrna nonratt021477 2 interference aggravates h2o2 induced oxidative stress in brl cells |
| topic | brl cells lncrna oxidative stress |
| url | https://vetdergikafkas.org/pdf.php?id=3153 |
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