Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry
A comprehensive analytical method was developed for the determination of diclazuril and toltrazuril residue makers in eggs by ultra-high performance liquid chromatography-atmospheric pressure chemical ionization-triple quadrupole mass spectrometry (UPLC-APCI-MS/MS). Samples were extracted with aceto...
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China Food Publishing Company
2025-04-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-8-035.pdf |
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| author | ZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru |
| author_facet | ZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru |
| author_sort | ZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru |
| collection | DOAJ |
| description | A comprehensive analytical method was developed for the determination of diclazuril and toltrazuril residue makers in eggs by ultra-high performance liquid chromatography-atmospheric pressure chemical ionization-triple quadrupole mass spectrometry (UPLC-APCI-MS/MS). Samples were extracted with acetonitrile and purified using a PRiME hydrophilic-lipophilic balance (HLB) column. Chromatographic separation was achieved on a ACQUITY BEH C18 column (2.1 mm × 100 mm, 1.7 µm) by gradient elution with a mobile phase composed of acetonitrile and 0.1% formic acid solution. An APCI interface was used as the ion source and the analysis was performed using negative ionization in multiple reaction monitoring (MRM) mode. An internal standard calibration curve method was employed for quantification. The calibration curves were linear in the range of 0.5–200 ng/mL with determination coefficients of more than 0.999 0. The limits of detection (LODs) were 0.5 μg/kg, and the limits of quantization (LOQs) were 2 μg/kg. The average recoveries of the analytes range from 72.8% to 106.5%, with relative standard deviations (RSDs) of 3.2%–8.5%. The developed method is simple, accurate, sensitive, and useful for the rapid detection and confirmation of diclazuride and toltrazuril sulfone residues in eggs. |
| format | Article |
| id | doaj-art-ae5f3f0ab20747f7bd4d26ede3c2e21c |
| institution | OA Journals |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-ae5f3f0ab20747f7bd4d26ede3c2e21c2025-08-20T02:12:18ZengChina Food Publishing CompanyShipin Kexue1002-66302025-04-0146833033910.7506/spkx1002-6630-20241113-099Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass SpectrometryZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru0(Shijiazhuang Customs Technology Center, Shijiazhuang 050051, China)A comprehensive analytical method was developed for the determination of diclazuril and toltrazuril residue makers in eggs by ultra-high performance liquid chromatography-atmospheric pressure chemical ionization-triple quadrupole mass spectrometry (UPLC-APCI-MS/MS). Samples were extracted with acetonitrile and purified using a PRiME hydrophilic-lipophilic balance (HLB) column. Chromatographic separation was achieved on a ACQUITY BEH C18 column (2.1 mm × 100 mm, 1.7 µm) by gradient elution with a mobile phase composed of acetonitrile and 0.1% formic acid solution. An APCI interface was used as the ion source and the analysis was performed using negative ionization in multiple reaction monitoring (MRM) mode. An internal standard calibration curve method was employed for quantification. The calibration curves were linear in the range of 0.5–200 ng/mL with determination coefficients of more than 0.999 0. The limits of detection (LODs) were 0.5 μg/kg, and the limits of quantization (LOQs) were 2 μg/kg. The average recoveries of the analytes range from 72.8% to 106.5%, with relative standard deviations (RSDs) of 3.2%–8.5%. The developed method is simple, accurate, sensitive, and useful for the rapid detection and confirmation of diclazuride and toltrazuril sulfone residues in eggs.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-8-035.pdfultra-high performance liquid chromatography-triple quadrupole mass spectrometry; atmospheric pressure chemical ionization; eggs; diclazuril; toltrazuril sulfone |
| spellingShingle | ZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry Shipin Kexue ultra-high performance liquid chromatography-triple quadrupole mass spectrometry; atmospheric pressure chemical ionization; eggs; diclazuril; toltrazuril sulfone |
| title | Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry |
| title_full | Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry |
| title_fullStr | Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry |
| title_full_unstemmed | Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry |
| title_short | Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry |
| title_sort | fast determination of diclazuril and toltrazuril residue makers in eggs by ultra high performance liquid chromatography atmospheric pressure chemical ionization triple quadrupole mass spectrometry |
| topic | ultra-high performance liquid chromatography-triple quadrupole mass spectrometry; atmospheric pressure chemical ionization; eggs; diclazuril; toltrazuril sulfone |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-8-035.pdf |
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