Characterisation of Clostridium difficile biofilm formation, a role for Spo0A.
Clostridium difficile is a Gram-positive anaerobic, spore-forming bacillus that is the leading cause of nosocomial diarrhoea worldwide. We demonstrate that C. difficile aggregates and forms biofilms in vitro on abiotic surfaces. These polymicrobial aggregates are attached to each other and to an abi...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050527&type=printable |
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| author | Lisa F Dawson Esmeralda Valiente Alexandra Faulds-Pain Elizabeth H Donahue Brendan W Wren |
| author_facet | Lisa F Dawson Esmeralda Valiente Alexandra Faulds-Pain Elizabeth H Donahue Brendan W Wren |
| author_sort | Lisa F Dawson |
| collection | DOAJ |
| description | Clostridium difficile is a Gram-positive anaerobic, spore-forming bacillus that is the leading cause of nosocomial diarrhoea worldwide. We demonstrate that C. difficile aggregates and forms biofilms in vitro on abiotic surfaces. These polymicrobial aggregates are attached to each other and to an abiotic surface by an extracellular polymeric substance (EPS). The EPS matrix provides the scaffold bonding together vegetative cells and spores, as well as forming a protective barrier for vegetative cells against oxygen stress. The master regulator of sporulation, Spo0A, may play a key role in biofilm formation, as genetic inactivation of spo0A in strain R20291 exhibits decreased biofilm formation. Our findings highlight an important attribute of C. difficile pathogenesis, which may have significant implications for infection, treatment and relapse. |
| format | Article |
| id | doaj-art-ae4c8b1a56774994b7f70f2baf58de48 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ae4c8b1a56774994b7f70f2baf58de482025-08-20T03:26:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5052710.1371/journal.pone.0050527Characterisation of Clostridium difficile biofilm formation, a role for Spo0A.Lisa F DawsonEsmeralda ValienteAlexandra Faulds-PainElizabeth H DonahueBrendan W WrenClostridium difficile is a Gram-positive anaerobic, spore-forming bacillus that is the leading cause of nosocomial diarrhoea worldwide. We demonstrate that C. difficile aggregates and forms biofilms in vitro on abiotic surfaces. These polymicrobial aggregates are attached to each other and to an abiotic surface by an extracellular polymeric substance (EPS). The EPS matrix provides the scaffold bonding together vegetative cells and spores, as well as forming a protective barrier for vegetative cells against oxygen stress. The master regulator of sporulation, Spo0A, may play a key role in biofilm formation, as genetic inactivation of spo0A in strain R20291 exhibits decreased biofilm formation. Our findings highlight an important attribute of C. difficile pathogenesis, which may have significant implications for infection, treatment and relapse.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050527&type=printable |
| spellingShingle | Lisa F Dawson Esmeralda Valiente Alexandra Faulds-Pain Elizabeth H Donahue Brendan W Wren Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. PLoS ONE |
| title | Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. |
| title_full | Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. |
| title_fullStr | Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. |
| title_full_unstemmed | Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. |
| title_short | Characterisation of Clostridium difficile biofilm formation, a role for Spo0A. |
| title_sort | characterisation of clostridium difficile biofilm formation a role for spo0a |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0050527&type=printable |
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