Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System
Angiogenesis is a key factor in early stages of wound healing and is crucial for the repair of vascularized tissues such as the bone. However, supporting timely revascularization of the defect site still presents a clinical challenge. Tissue engineering approaches delivering endothelial cells or pre...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/1075975 |
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| _version_ | 1850177055876972544 |
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| author | Jessica Zahn Markus Loibl Christoph Sprecher Michael Nerlich Mauro Alini Sophie Verrier Marietta Herrmann |
| author_facet | Jessica Zahn Markus Loibl Christoph Sprecher Michael Nerlich Mauro Alini Sophie Verrier Marietta Herrmann |
| author_sort | Jessica Zahn |
| collection | DOAJ |
| description | Angiogenesis is a key factor in early stages of wound healing and is crucial for the repair of vascularized tissues such as the bone. However, supporting timely revascularization of the defect site still presents a clinical challenge. Tissue engineering approaches delivering endothelial cells or prevascularized constructs may overcome this problem. In the current study, we investigated platelet-rich plasma (PRP) gels as autologous, injectable cell delivery systems for prevascularized constructs. PRP was produced from human thrombocyte concentrates. GFP-expressing human umbilical vein endothelial cells (HUVECs) and human bone marrow-derived mesenchymal stem cells (MSCs) were encapsulated in PRP gels in different proportions. The formation of cellular networks was assessed over 14 days by time-lapse microscopy, gene expression analysis, and immunohistology. PRP gels presented a favorable environment for the formation of a three-dimensional (3D) cellular network. The formation of these networks was apparent as early as 3 days after seeding. Networks increased in complexity and branching over time but were only stable in HUVEC-MSC cocultures. The high cell viability together with the 3D capillary-like networks observed at early time points suggests that PRP can be used as an autologous and proangiogenic cell delivery system for the repair of vascularized tissues such as the bone. |
| format | Article |
| id | doaj-art-ae4669e9349a4feebdb97d36608c19b5 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ae4669e9349a4feebdb97d36608c19b52025-08-20T02:19:05ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/10759751075975Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery SystemJessica Zahn0Markus Loibl1Christoph Sprecher2Michael Nerlich3Mauro Alini4Sophie Verrier5Marietta Herrmann6AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandDepartment of Trauma Surgery, University Medical Center Regensburg, Regensburg, GermanyAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandDepartment of Trauma Surgery, University Medical Center Regensburg, Regensburg, GermanyAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandAngiogenesis is a key factor in early stages of wound healing and is crucial for the repair of vascularized tissues such as the bone. However, supporting timely revascularization of the defect site still presents a clinical challenge. Tissue engineering approaches delivering endothelial cells or prevascularized constructs may overcome this problem. In the current study, we investigated platelet-rich plasma (PRP) gels as autologous, injectable cell delivery systems for prevascularized constructs. PRP was produced from human thrombocyte concentrates. GFP-expressing human umbilical vein endothelial cells (HUVECs) and human bone marrow-derived mesenchymal stem cells (MSCs) were encapsulated in PRP gels in different proportions. The formation of cellular networks was assessed over 14 days by time-lapse microscopy, gene expression analysis, and immunohistology. PRP gels presented a favorable environment for the formation of a three-dimensional (3D) cellular network. The formation of these networks was apparent as early as 3 days after seeding. Networks increased in complexity and branching over time but were only stable in HUVEC-MSC cocultures. The high cell viability together with the 3D capillary-like networks observed at early time points suggests that PRP can be used as an autologous and proangiogenic cell delivery system for the repair of vascularized tissues such as the bone.http://dx.doi.org/10.1155/2017/1075975 |
| spellingShingle | Jessica Zahn Markus Loibl Christoph Sprecher Michael Nerlich Mauro Alini Sophie Verrier Marietta Herrmann Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System Mediators of Inflammation |
| title | Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System |
| title_full | Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System |
| title_fullStr | Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System |
| title_full_unstemmed | Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System |
| title_short | Platelet-Rich Plasma as an Autologous and Proangiogenic Cell Delivery System |
| title_sort | platelet rich plasma as an autologous and proangiogenic cell delivery system |
| url | http://dx.doi.org/10.1155/2017/1075975 |
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