Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study
Abstract Intravenous leiomyomatosis (IVL) is a histologically well differentiated smooth muscle tumor with aggressive behavior, capable of extending throughout the venous system. Understanding how IVL occurs and develops is really important for diagnosing and treating it. Unfortunately, because IVL...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-02-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-024-05919-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850045699993894912 |
|---|---|
| author | Sheng Yin Peipei Shi Jing Han Hua Li Aimin Ren Li Ma Wenbin Tang Wenxue Liu Sihui Yu Tingting Li Chunsheng Wang Yingyong Hou Jiarong Zhang |
| author_facet | Sheng Yin Peipei Shi Jing Han Hua Li Aimin Ren Li Ma Wenbin Tang Wenxue Liu Sihui Yu Tingting Li Chunsheng Wang Yingyong Hou Jiarong Zhang |
| author_sort | Sheng Yin |
| collection | DOAJ |
| description | Abstract Intravenous leiomyomatosis (IVL) is a histologically well differentiated smooth muscle tumor with aggressive behavior, capable of extending throughout the venous system. Understanding how IVL occurs and develops is really important for diagnosing and treating it. Unfortunately, because IVL is quite rare, there aren’t many comprehensive studies available. In our research, we carried out an extensive multi-omics study, gathering tissue samples from IVL cases, uterine fibroid, and normal myometrium. The single-cell RNA sequencing analysis revealed a notable difference in cell composition between IVL and uterine fibroid. Additionally, H&E staining demonstrated more frequent hydropic changes and hyalinization in IVL tissues, along with a reduced vascular density compared to both normal myometrium and uterine fibroid. In our proteomics analysis of eight paired samples of IVL and normal myometrium fresh frozen tissue, we identified proteins that were differentially expressed, mainly related to focal adhesions and regulation of the actin cytoskeleton. The most frequently involved chromosomes included deletions in 10q22.2, 10q24.32, 13q14, and 13q21-31. Correlation analyses highlighted chromosome 10q as the most frequent cytoband, with corresponding proteins involved in regulating focal adhesions and the cytoskeleton. Integrated analysis between pathological and clinical characteristics indicated that chromosome 10q deletion and vascular morphology in IVL could serve as important markers predicting aggressive behavior. Our study sheds light on the pathological and molecular changes linked to IVL, which could pave the way for new treatment approaches. |
| format | Article |
| id | doaj-art-ae2f549f4e404cbc8def24493cc4492d |
| institution | DOAJ |
| issn | 1479-5876 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj-art-ae2f549f4e404cbc8def24493cc4492d2025-08-20T02:54:37ZengBMCJournal of Translational Medicine1479-58762025-02-0123111410.1186/s12967-024-05919-9Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics studySheng Yin0Peipei Shi1Jing Han2Hua Li3Aimin Ren4Li Ma5Wenbin Tang6Wenxue Liu7Sihui Yu8Tingting Li9Chunsheng Wang10Yingyong Hou11Jiarong Zhang12Department of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Pathology, Zhongshan Hospital, Fudan UniversityDepartment of Cardiac Surgery, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityDepartment of Cardiac Surgery, Zhongshan Hospital, Fudan UniversityDepartment of Pathology, Zhongshan Hospital, Fudan UniversityDepartment of Obstetrics and Gynecology, Zhongshan Hospital, Fudan UniversityAbstract Intravenous leiomyomatosis (IVL) is a histologically well differentiated smooth muscle tumor with aggressive behavior, capable of extending throughout the venous system. Understanding how IVL occurs and develops is really important for diagnosing and treating it. Unfortunately, because IVL is quite rare, there aren’t many comprehensive studies available. In our research, we carried out an extensive multi-omics study, gathering tissue samples from IVL cases, uterine fibroid, and normal myometrium. The single-cell RNA sequencing analysis revealed a notable difference in cell composition between IVL and uterine fibroid. Additionally, H&E staining demonstrated more frequent hydropic changes and hyalinization in IVL tissues, along with a reduced vascular density compared to both normal myometrium and uterine fibroid. In our proteomics analysis of eight paired samples of IVL and normal myometrium fresh frozen tissue, we identified proteins that were differentially expressed, mainly related to focal adhesions and regulation of the actin cytoskeleton. The most frequently involved chromosomes included deletions in 10q22.2, 10q24.32, 13q14, and 13q21-31. Correlation analyses highlighted chromosome 10q as the most frequent cytoband, with corresponding proteins involved in regulating focal adhesions and the cytoskeleton. Integrated analysis between pathological and clinical characteristics indicated that chromosome 10q deletion and vascular morphology in IVL could serve as important markers predicting aggressive behavior. Our study sheds light on the pathological and molecular changes linked to IVL, which could pave the way for new treatment approaches.https://doi.org/10.1186/s12967-024-05919-9Intravenous leiomyomatosisMulti-omics analysisChromosome 10q deletionVascular morphologyAggressive behavior |
| spellingShingle | Sheng Yin Peipei Shi Jing Han Hua Li Aimin Ren Li Ma Wenbin Tang Wenxue Liu Sihui Yu Tingting Li Chunsheng Wang Yingyong Hou Jiarong Zhang Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study Journal of Translational Medicine Intravenous leiomyomatosis Multi-omics analysis Chromosome 10q deletion Vascular morphology Aggressive behavior |
| title | Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study |
| title_full | Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study |
| title_fullStr | Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study |
| title_full_unstemmed | Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study |
| title_short | Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study |
| title_sort | pathological and molecular insights into intravenous leiomyomatosis an integrative multi omics study |
| topic | Intravenous leiomyomatosis Multi-omics analysis Chromosome 10q deletion Vascular morphology Aggressive behavior |
| url | https://doi.org/10.1186/s12967-024-05919-9 |
| work_keys_str_mv | AT shengyin pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT peipeishi pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT jinghan pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT huali pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT aiminren pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT lima pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT wenbintang pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT wenxueliu pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT sihuiyu pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT tingtingli pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT chunshengwang pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT yingyonghou pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy AT jiarongzhang pathologicalandmolecularinsightsintointravenousleiomyomatosisanintegrativemultiomicsstudy |