Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm
IntroductionThe livestock industry has witnessed a notable increase in the proportion of male calves born following artificial insemination with cryopreserved semen, hinting at a possible association between cryotolerance and inherent structural or functional disparities between X- and Y-chromosome...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1582961/full |
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| author | Jieru Wang Jieru Wang Ruchun Li Fei Huang Peng Niu Yanling Zheng Dongfang Yuan Yan Du Congcong Li Mingcai Yang Jiajia Suo Jiajia Suo Qinghua Gao Qinghua Gao |
| author_facet | Jieru Wang Jieru Wang Ruchun Li Fei Huang Peng Niu Yanling Zheng Dongfang Yuan Yan Du Congcong Li Mingcai Yang Jiajia Suo Jiajia Suo Qinghua Gao Qinghua Gao |
| author_sort | Jieru Wang |
| collection | DOAJ |
| description | IntroductionThe livestock industry has witnessed a notable increase in the proportion of male calves born following artificial insemination with cryopreserved semen, hinting at a possible association between cryotolerance and inherent structural or functional disparities between X- and Y-chromosome bearing sperm. To delve into this phenomenon, we conducted a comprehensive proteomic analysis on bovine sex-sorted semen.MethodsSperm samples were meticulously categorized based on stringent quality parameters. Concurrent sorting of X- and Y-sperm from identical ejaculates was performed, followed by protein extraction for subsequent analysis. Quantitative isobaric tags for relative and absolute quantification (iTRAQ) were employed to identify differentially expressed proteins.ResultsiTRAQ pinpointed 20 proteins with differential expression between X- and Y-spermatozoa, including 12 upregulated and 8 downregulated proteins in Y-sperm. Aquaporin 7 (AQP7), significantly upregulated in Y-sperm, was identified as a key candidate. Western blot analysis corroborated elevated AQP7 expression in Y-sperm compared to X-sperm, while Aquaporin 3 (AQP3) showed no significant difference. Immunofluorescence revealed AQP7 localization to the post-acrosomal region, midpiece, and principal piece of Y-sperm with higher fluorescence intensity, whereas AQP3 distribution was comparable between groups.DiscussionThese findings imply a potential role for AQP7 in augmenting Y-sperm cryotolerance, providing molecular insights into sex-specific cryopreservation effects on fertility outcomes. Future research should elucidate AQP7’s functional mechanisms and explore implications for livestock breeding technologies. |
| format | Article |
| id | doaj-art-ae281b27ea92427e83337381e9ef95ca |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-ae281b27ea92427e83337381e9ef95ca2025-08-20T03:49:46ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-05-011310.3389/fcell.2025.15829611582961Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine spermJieru Wang0Jieru Wang1Ruchun Li2Fei Huang3Peng Niu4Yanling Zheng5Dongfang Yuan6Yan Du7Congcong Li8Mingcai Yang9Jiajia Suo10Jiajia Suo11Qinghua Gao12Qinghua Gao13Department of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaCollege of Life Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaCollege of Life Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaCollege of Life Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaDepartment of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, ChinaCollege of Life Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaCollege of Life Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaCollege of Animal Sciences and Technology, Tarim University, Alar, Xinjiang, ChinaIntroductionThe livestock industry has witnessed a notable increase in the proportion of male calves born following artificial insemination with cryopreserved semen, hinting at a possible association between cryotolerance and inherent structural or functional disparities between X- and Y-chromosome bearing sperm. To delve into this phenomenon, we conducted a comprehensive proteomic analysis on bovine sex-sorted semen.MethodsSperm samples were meticulously categorized based on stringent quality parameters. Concurrent sorting of X- and Y-sperm from identical ejaculates was performed, followed by protein extraction for subsequent analysis. Quantitative isobaric tags for relative and absolute quantification (iTRAQ) were employed to identify differentially expressed proteins.ResultsiTRAQ pinpointed 20 proteins with differential expression between X- and Y-spermatozoa, including 12 upregulated and 8 downregulated proteins in Y-sperm. Aquaporin 7 (AQP7), significantly upregulated in Y-sperm, was identified as a key candidate. Western blot analysis corroborated elevated AQP7 expression in Y-sperm compared to X-sperm, while Aquaporin 3 (AQP3) showed no significant difference. Immunofluorescence revealed AQP7 localization to the post-acrosomal region, midpiece, and principal piece of Y-sperm with higher fluorescence intensity, whereas AQP3 distribution was comparable between groups.DiscussionThese findings imply a potential role for AQP7 in augmenting Y-sperm cryotolerance, providing molecular insights into sex-specific cryopreservation effects on fertility outcomes. Future research should elucidate AQP7’s functional mechanisms and explore implications for livestock breeding technologies.https://www.frontiersin.org/articles/10.3389/fcell.2025.1582961/fullAQP7AQP3cryotolerance of spermX-spermY-sperm |
| spellingShingle | Jieru Wang Jieru Wang Ruchun Li Fei Huang Peng Niu Yanling Zheng Dongfang Yuan Yan Du Congcong Li Mingcai Yang Jiajia Suo Jiajia Suo Qinghua Gao Qinghua Gao Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm Frontiers in Cell and Developmental Biology AQP7 AQP3 cryotolerance of sperm X-sperm Y-sperm |
| title | Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm |
| title_full | Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm |
| title_fullStr | Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm |
| title_full_unstemmed | Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm |
| title_short | Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm |
| title_sort | comparative analysis of aqp7 expression and cryotolerance in x and y chromosome bearing bovine sperm |
| topic | AQP7 AQP3 cryotolerance of sperm X-sperm Y-sperm |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1582961/full |
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