Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor
The presence of Hg2+ causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg2+ in plant tissues is crucial for the management of Hg pollution;...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-01-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0570 |
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| author | Sumeera Asghar Zhenyang Yu Zheng Zhu Dengyue Zheng Zimo Zhao Yuming Xu Xiao Liu Chao Yuan Yan Li Wei Wang Jianfeng Xu Huailong Teng Jun Li Wen-Chao Yang Chunli Chen |
| author_facet | Sumeera Asghar Zhenyang Yu Zheng Zhu Dengyue Zheng Zimo Zhao Yuming Xu Xiao Liu Chao Yuan Yan Li Wei Wang Jianfeng Xu Huailong Teng Jun Li Wen-Chao Yang Chunli Chen |
| author_sort | Sumeera Asghar |
| collection | DOAJ |
| description | The presence of Hg2+ causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg2+ in plant tissues is crucial for the management of Hg pollution; however, the related research is still at its early stage. Herein, a small-molecule amphiphilic fluorescent probe (termed LJTP2) was developed for the specific detection of Hg2+ with a high sensitivity (~16 nM). Fluorescent imaging applications with LJTP2 not only detected the dynamic distribution of Hg2+ within plant cells at the subcellular level but also enabled the understanding of cell membrane health under Hg2+ stress. This study introduces a valuable imaging tool for elucidating the molecular mechanism of Hg2+ stress in plants, demonstrating the potential of the application of small-molecule fluorescent probes in plant science. |
| format | Article |
| id | doaj-art-ae13822d699f4d9f85e964d004d58d59 |
| institution | Kabale University |
| issn | 2639-5274 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj-art-ae13822d699f4d9f85e964d004d58d592025-01-07T08:00:37ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742025-01-01810.34133/research.0570Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent SensorSumeera Asghar0Zhenyang Yu1Zheng Zhu2Dengyue Zheng3Zimo Zhao4Yuming Xu5Xiao Liu6Chao Yuan7Yan Li8Wei Wang9Jianfeng Xu10Huailong Teng11Jun Li12Wen-Chao Yang13Chunli Chen14State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.The Key Laboratory of Plant Resources Conservation Germplasm Innovation in Mountainous Region, College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China.State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.The presence of Hg2+ causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg2+ in plant tissues is crucial for the management of Hg pollution; however, the related research is still at its early stage. Herein, a small-molecule amphiphilic fluorescent probe (termed LJTP2) was developed for the specific detection of Hg2+ with a high sensitivity (~16 nM). Fluorescent imaging applications with LJTP2 not only detected the dynamic distribution of Hg2+ within plant cells at the subcellular level but also enabled the understanding of cell membrane health under Hg2+ stress. This study introduces a valuable imaging tool for elucidating the molecular mechanism of Hg2+ stress in plants, demonstrating the potential of the application of small-molecule fluorescent probes in plant science.https://spj.science.org/doi/10.34133/research.0570 |
| spellingShingle | Sumeera Asghar Zhenyang Yu Zheng Zhu Dengyue Zheng Zimo Zhao Yuming Xu Xiao Liu Chao Yuan Yan Li Wei Wang Jianfeng Xu Huailong Teng Jun Li Wen-Chao Yang Chunli Chen Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor Research |
| title | Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor |
| title_full | Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor |
| title_fullStr | Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor |
| title_full_unstemmed | Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor |
| title_short | Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor |
| title_sort | visualization of hg2 stress on plant health at the subcellular level revealed by a highly sensitive fluorescent sensor |
| url | https://spj.science.org/doi/10.34133/research.0570 |
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