Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway
Drought poses a significant challenge to global potato production. Grafting, a classical horticultural technique, has the potential to enhance resistance to both biotic and abiotic stresses. However, the use of grafting to improve drought resistance in potatoes, along with the underlying genetic and...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X2400318X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850249401219416064 |
|---|---|
| author | Yinqiao Jian Chunyan Gao Yangyang Shang Junhong Qin Shaoguang Duan Chunsong Bian Guangcun Li |
| author_facet | Yinqiao Jian Chunyan Gao Yangyang Shang Junhong Qin Shaoguang Duan Chunsong Bian Guangcun Li |
| author_sort | Yinqiao Jian |
| collection | DOAJ |
| description | Drought poses a significant challenge to global potato production. Grafting, a classical horticultural technique, has the potential to enhance resistance to both biotic and abiotic stresses. However, the use of grafting to improve drought resistance in potatoes, along with the underlying genetic and regulatory changes remains inadequately documented. In this study, we investigated the drought phenotypes, as well as the metabolomic and transcriptomic profiles of leaves and roots in self-grafted (drought-sensitive scion/ drought-sensitive rootstock, SS) and hetero-grafted (drought-sensitive scion/drought-tolerant rootstock, ST) potatoes. After 40 days, hetero-grafts exhibited greater drought resistance as well as lower dry matter content and higher soluble sugar content compared to self-grafts, indicating that grafting with drought-tolerant rootstocks can enhance the drought resistance of the scion and revealed physiological process. Metabolomic analysis revealed a significant enrichment of flavonoids, particularly in comparisons between SS-leaf vs. ST-leaf and SS-root vs. ST-root. Transcriptomic analysis further supported these findings, showing an enrichment in the biosynthesis of plant secondary metabolites in the same comparisons, aligning with metabolomic data. These differentially accumulated metabolites and expressed genes, particularly in SS-leaf vs. ST-leaf comparison, suggest a mechanism involving long-distance metabolites and mRNA in grafting-mediated drought resistance. Weighted Gene Co-expression Network Analysis identified the yellow module, which correlated with drought, and highlighted MYB or MYB-related genes as hub genes. Our results reveal global metabolomic and transcriptomic features associated with drought tolerance in potatoes, demonstrating that grafting can alter the composition and accumulation of genes and metabolites, leading to enhanced drought resistance. The significant role of flavonoids as modulators of drought resistance, supported by comprehensive transcriptomic and metabolomic analyses, underscores the pivotal regulatory function of the MYB-WD40-bHLH transcription factor complex in orchestrating the stress response. |
| format | Article |
| id | doaj-art-ae083ab2cb8f4558b7a9fdea611b43e4 |
| institution | OA Journals |
| issn | 2667-064X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-ae083ab2cb8f4558b7a9fdea611b43e42025-08-20T01:58:30ZengElsevierPlant Stress2667-064X2024-12-011410066510.1016/j.stress.2024.100665Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathwayYinqiao Jian0Chunyan Gao1Yangyang Shang2Junhong Qin3Shaoguang Duan4Chunsong Bian5Guangcun Li6State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaState Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaState Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaState Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaState Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaState Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaCorresponding author at: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12# Zhongguancun Nandajie, Haidian District Beijing, 100081.; State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR ChinaDrought poses a significant challenge to global potato production. Grafting, a classical horticultural technique, has the potential to enhance resistance to both biotic and abiotic stresses. However, the use of grafting to improve drought resistance in potatoes, along with the underlying genetic and regulatory changes remains inadequately documented. In this study, we investigated the drought phenotypes, as well as the metabolomic and transcriptomic profiles of leaves and roots in self-grafted (drought-sensitive scion/ drought-sensitive rootstock, SS) and hetero-grafted (drought-sensitive scion/drought-tolerant rootstock, ST) potatoes. After 40 days, hetero-grafts exhibited greater drought resistance as well as lower dry matter content and higher soluble sugar content compared to self-grafts, indicating that grafting with drought-tolerant rootstocks can enhance the drought resistance of the scion and revealed physiological process. Metabolomic analysis revealed a significant enrichment of flavonoids, particularly in comparisons between SS-leaf vs. ST-leaf and SS-root vs. ST-root. Transcriptomic analysis further supported these findings, showing an enrichment in the biosynthesis of plant secondary metabolites in the same comparisons, aligning with metabolomic data. These differentially accumulated metabolites and expressed genes, particularly in SS-leaf vs. ST-leaf comparison, suggest a mechanism involving long-distance metabolites and mRNA in grafting-mediated drought resistance. Weighted Gene Co-expression Network Analysis identified the yellow module, which correlated with drought, and highlighted MYB or MYB-related genes as hub genes. Our results reveal global metabolomic and transcriptomic features associated with drought tolerance in potatoes, demonstrating that grafting can alter the composition and accumulation of genes and metabolites, leading to enhanced drought resistance. The significant role of flavonoids as modulators of drought resistance, supported by comprehensive transcriptomic and metabolomic analyses, underscores the pivotal regulatory function of the MYB-WD40-bHLH transcription factor complex in orchestrating the stress response.http://www.sciencedirect.com/science/article/pii/S2667064X2400318XPotatoDroughtGraftMetabolomicTranscriptomicFlavonoid |
| spellingShingle | Yinqiao Jian Chunyan Gao Yangyang Shang Junhong Qin Shaoguang Duan Chunsong Bian Guangcun Li Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway Plant Stress Potato Drought Graft Metabolomic Transcriptomic Flavonoid |
| title | Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| title_full | Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| title_fullStr | Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| title_full_unstemmed | Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| title_short | Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| title_sort | metabolomic and transcriptomic analyses reveal myb related genes involved in drought resistance in grafted potatoes via the flavonoid pathway |
| topic | Potato Drought Graft Metabolomic Transcriptomic Flavonoid |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X2400318X |
| work_keys_str_mv | AT yinqiaojian metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT chunyangao metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT yangyangshang metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT junhongqin metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT shaoguangduan metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT chunsongbian metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway AT guangcunli metabolomicandtranscriptomicanalysesrevealmybrelatedgenesinvolvedindroughtresistanceingraftedpotatoesviatheflavonoidpathway |