Towards a quality control framework for cerebral cortical organoids
Abstract Cerebral organoids offer significant potential for neuroscience research as complex in vitro models that mimic human brain development. However, challenges related to their quality and reproducibility hinder their reliability. Discrepancies in morphology, size, cellular composition, and cyt...
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Nature Portfolio
2025-08-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-14425-x |
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| author | Héloïse Castiglione Lucie Madrange Camille Baquerre Benoît Guy Christian Maisonneuve Thomas Lemonnier Jean-Philippe Deslys Frank Yates Thibault Honegger Jessica Rontard Pierre-Antoine Vigneron |
| author_facet | Héloïse Castiglione Lucie Madrange Camille Baquerre Benoît Guy Christian Maisonneuve Thomas Lemonnier Jean-Philippe Deslys Frank Yates Thibault Honegger Jessica Rontard Pierre-Antoine Vigneron |
| author_sort | Héloïse Castiglione |
| collection | DOAJ |
| description | Abstract Cerebral organoids offer significant potential for neuroscience research as complex in vitro models that mimic human brain development. However, challenges related to their quality and reproducibility hinder their reliability. Discrepancies in morphology, size, cellular composition, and cytoarchitectural organization limit their applications, particularly in disease modeling, drug screening, and neurotoxicity testing. Critically, current methods for organoid characterization often lack standardization, restricting their broader applicability. To address the need for standardized quality assessment of cerebral organoids, we developed a Quality Control (QC) methodology for 60-day cortical organoids, evaluating five key criteria using a scoring system: morphology, size and growth profile, cellular composition, cytoarchitectural organization, and cytotoxicity. We implemented a hierarchical approach, beginning with non-invasive assessments to exclude low-quality organoids, while reserving in-depth analyses for those that passed the initial evaluation. To validate this framework, we exposed 60-day cortical organoids to graded doses of hydrogen peroxide (H2O2), inducing a range of quality outcomes. The QC system demonstrated its robustness by accurately discriminating organoid qualities. Our proposed QC framework is designed to be user-friendly, flexible, and broadly applicable, making it suitable for routine assessment of cerebral organoid quality. Additionally, its scalability enables industrial applications, offering a valuable tool for advancing both fundamental and pre-clinical research. |
| format | Article |
| id | doaj-art-ae01854be7f44848bcce293ffe9c9d14 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ae01854be7f44848bcce293ffe9c9d142025-08-20T03:42:49ZengNature PortfolioScientific Reports2045-23222025-08-0115111710.1038/s41598-025-14425-xTowards a quality control framework for cerebral cortical organoidsHéloïse Castiglione0Lucie Madrange1Camille Baquerre2Benoît Guy Christian Maisonneuve3Thomas Lemonnier4Jean-Philippe Deslys5Frank Yates6Thibault Honegger7Jessica Rontard8Pierre-Antoine Vigneron9SupBiotech, Ecole d’Ingénieurs en BiotechnologiesSupBiotech, Ecole d’Ingénieurs en BiotechnologiesNETRINETRISupBiotech, Ecole d’Ingénieurs en BiotechnologiesCommissariat à l’Energie Atomique et aux Energies Alternatives (CEA), Service d’Etude des Prions et des Infections Atypiques (SEPIA), Université Paris-SaclaySupBiotech, Ecole d’Ingénieurs en BiotechnologiesNETRINETRISupBiotech, Ecole d’Ingénieurs en BiotechnologiesAbstract Cerebral organoids offer significant potential for neuroscience research as complex in vitro models that mimic human brain development. However, challenges related to their quality and reproducibility hinder their reliability. Discrepancies in morphology, size, cellular composition, and cytoarchitectural organization limit their applications, particularly in disease modeling, drug screening, and neurotoxicity testing. Critically, current methods for organoid characterization often lack standardization, restricting their broader applicability. To address the need for standardized quality assessment of cerebral organoids, we developed a Quality Control (QC) methodology for 60-day cortical organoids, evaluating five key criteria using a scoring system: morphology, size and growth profile, cellular composition, cytoarchitectural organization, and cytotoxicity. We implemented a hierarchical approach, beginning with non-invasive assessments to exclude low-quality organoids, while reserving in-depth analyses for those that passed the initial evaluation. To validate this framework, we exposed 60-day cortical organoids to graded doses of hydrogen peroxide (H2O2), inducing a range of quality outcomes. The QC system demonstrated its robustness by accurately discriminating organoid qualities. Our proposed QC framework is designed to be user-friendly, flexible, and broadly applicable, making it suitable for routine assessment of cerebral organoid quality. Additionally, its scalability enables industrial applications, offering a valuable tool for advancing both fundamental and pre-clinical research.https://doi.org/10.1038/s41598-025-14425-xCerebral organoidsQuality controlHierarchical scoring methodologyReproducibilityStandardization. |
| spellingShingle | Héloïse Castiglione Lucie Madrange Camille Baquerre Benoît Guy Christian Maisonneuve Thomas Lemonnier Jean-Philippe Deslys Frank Yates Thibault Honegger Jessica Rontard Pierre-Antoine Vigneron Towards a quality control framework for cerebral cortical organoids Scientific Reports Cerebral organoids Quality control Hierarchical scoring methodology Reproducibility Standardization. |
| title | Towards a quality control framework for cerebral cortical organoids |
| title_full | Towards a quality control framework for cerebral cortical organoids |
| title_fullStr | Towards a quality control framework for cerebral cortical organoids |
| title_full_unstemmed | Towards a quality control framework for cerebral cortical organoids |
| title_short | Towards a quality control framework for cerebral cortical organoids |
| title_sort | towards a quality control framework for cerebral cortical organoids |
| topic | Cerebral organoids Quality control Hierarchical scoring methodology Reproducibility Standardization. |
| url | https://doi.org/10.1038/s41598-025-14425-x |
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