Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses
Superoxide dismutases (SODs) serve as the first line of defense against reactive oxygen species. Copper-zinc superoxide dismutase (Cu/Zn-SOD) is an enzyme whose activity depends on copper availability. Cu/Zn-SOD-1 induction is shown to be involved in the antioxidative and antiviral activity of acety...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1561731/full |
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| author | Haijuan Wang Jidan Zhang Zhuo Meng Zhenqi Sun Dongyang Liu Bin Li Fangfang Yan Chongyi Jia Hongyou Zhou Hongyou Zhou Mingmin Zhao Mingmin Zhao |
| author_facet | Haijuan Wang Jidan Zhang Zhuo Meng Zhenqi Sun Dongyang Liu Bin Li Fangfang Yan Chongyi Jia Hongyou Zhou Hongyou Zhou Mingmin Zhao Mingmin Zhao |
| author_sort | Haijuan Wang |
| collection | DOAJ |
| description | Superoxide dismutases (SODs) serve as the first line of defense against reactive oxygen species. Copper-zinc superoxide dismutase (Cu/Zn-SOD) is an enzyme whose activity depends on copper availability. Cu/Zn-SOD-1 induction is shown to be involved in the antioxidative and antiviral activity of acetylsalicylic acid in hepatitis C virus (HCV)-expressing cells. Here, RNA sequencing (RNA-seq) analysis identified SOD homologs (three NbCu/Zn-SOD, four NbFe-SOD, and two NbMn-SOD) that were differentially expressed in Nicotiana benthamiana during tobacco vein mottling virus (TVMV) infection. NbCu/Zn-SOD-1 was cloned from N. benthamiana and subsequently characterized. Encoding sequence and structural analyses of the NbCu/Zn-SOD-1 protein confirmed a conserved SOD enzyme domain, GFHLHEfGDtT, indicating that it is SOD-dependent and phylogenetically related to Cu/Zn-SOD in Nicotiana tabacum (XP 016486719.1). NbCu/Zn-SOD-1 was primarily localized in the cytoplasm. The transient expression of NbCu/Zn-SOD-1 led to a reduced accumulation of TVMV and PVY-Rosea1 (PVY-Ros1). In summary, our results suggest that NbCu/Zn-SOD-1 homologs participate in plant antiviral responses. |
| format | Article |
| id | doaj-art-adffc83eb6a245fca5517372de155b53 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-adffc83eb6a245fca5517372de155b532025-08-20T03:12:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15617311561731Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responsesHaijuan Wang0Jidan Zhang1Zhuo Meng2Zhenqi Sun3Dongyang Liu4Bin Li5Fangfang Yan6Chongyi Jia7Hongyou Zhou8Hongyou Zhou9Mingmin Zhao10Mingmin Zhao11College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaSichuan Province Company of Tobacco Corporation in China, Chengdu, ChinaSichuan Province Company of Tobacco Corporation in China, Chengdu, ChinaSichuan Province Company of Tobacco Corporation in China, Chengdu, ChinaAgriculture and Animal Husbandry Bureau of Bayannaoer City, Inner Mongolia (Rural Vitalization Bureau), Bayannaoer, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of the Development and Resource Utilization of Biological Pesticide in Inner Mongolia, Hohhot, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of the Development and Resource Utilization of Biological Pesticide in Inner Mongolia, Hohhot, ChinaSuperoxide dismutases (SODs) serve as the first line of defense against reactive oxygen species. Copper-zinc superoxide dismutase (Cu/Zn-SOD) is an enzyme whose activity depends on copper availability. Cu/Zn-SOD-1 induction is shown to be involved in the antioxidative and antiviral activity of acetylsalicylic acid in hepatitis C virus (HCV)-expressing cells. Here, RNA sequencing (RNA-seq) analysis identified SOD homologs (three NbCu/Zn-SOD, four NbFe-SOD, and two NbMn-SOD) that were differentially expressed in Nicotiana benthamiana during tobacco vein mottling virus (TVMV) infection. NbCu/Zn-SOD-1 was cloned from N. benthamiana and subsequently characterized. Encoding sequence and structural analyses of the NbCu/Zn-SOD-1 protein confirmed a conserved SOD enzyme domain, GFHLHEfGDtT, indicating that it is SOD-dependent and phylogenetically related to Cu/Zn-SOD in Nicotiana tabacum (XP 016486719.1). NbCu/Zn-SOD-1 was primarily localized in the cytoplasm. The transient expression of NbCu/Zn-SOD-1 led to a reduced accumulation of TVMV and PVY-Rosea1 (PVY-Ros1). In summary, our results suggest that NbCu/Zn-SOD-1 homologs participate in plant antiviral responses.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1561731/fullsuperoxide dismutasesNbCu/Zn-SOD-1Nicotiana benthamianatobacco vein mottling virusantiviral resistance |
| spellingShingle | Haijuan Wang Jidan Zhang Zhuo Meng Zhenqi Sun Dongyang Liu Bin Li Fangfang Yan Chongyi Jia Hongyou Zhou Hongyou Zhou Mingmin Zhao Mingmin Zhao Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses Frontiers in Microbiology superoxide dismutases NbCu/Zn-SOD-1 Nicotiana benthamiana tobacco vein mottling virus antiviral resistance |
| title | Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses |
| title_full | Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses |
| title_fullStr | Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses |
| title_full_unstemmed | Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses |
| title_short | Cu/Zn superoxide dismutase homologs participate in Nicotiana benthamiana antiviral responses |
| title_sort | cu zn superoxide dismutase homologs participate in nicotiana benthamiana antiviral responses |
| topic | superoxide dismutases NbCu/Zn-SOD-1 Nicotiana benthamiana tobacco vein mottling virus antiviral resistance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1561731/full |
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