Expression regulation of bacterial lipase genes: a review

Microbial lipases constitute the primary source of commercialized and industrial lipases, and they are extensively utilized across numerous industrial sectors. Compared to fungal lipases, bacterial lipases catalyze a broader spectrum of reactions with higher activity, enhanced stability, and improve...

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Main Authors: Dai-ming Zha, Yun-jun Yan
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1592059/full
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author Dai-ming Zha
Yun-jun Yan
author_facet Dai-ming Zha
Yun-jun Yan
author_sort Dai-ming Zha
collection DOAJ
description Microbial lipases constitute the primary source of commercialized and industrial lipases, and they are extensively utilized across numerous industrial sectors. Compared to fungal lipases, bacterial lipases catalyze a broader spectrum of reactions with higher activity, enhanced stability, and improved stress resistance. Among them, lipases from Pseudomonas and Burkholderia cepacia are among the most widely employed microbial lipases. Furthermore, bacterial extracellular lipases act as crucial virulence factors, playing a significant role in the pathogenesis of bacteria. However, the production of bacterial lipases is typically low, rendering them expensive in the market and insufficient to meet the substantial demand for industrial production. To achieve large-scale production of bacterial lipases, stable and efficient homologous expression has proven to be an effective strategy. However, elucidating how bacterial lipase genes are regulated is the initial step for developing stable and efficient homologous expression, and a pressing scientific challenge. To date, the regulatory mechanisms governing the expression of bacterial lipase genes remain unclear, significantly impeding the construction of robust and high-yield homologous expression systems. Concurrently, understanding these regulatory mechanisms can facilitate early diagnosis of lipase-related pathogenic bacterial infections, and aid in the development of novel antibacterial drugs. In this review, we summarized the advancements in understanding the expression regulation of bacterial lipase genes, including direct regulators, the quorum sensing (QS) system, the Gac/Rsm system and its related regulators, as well as other regulators. Additionally, based on our ongoing research, we also discussed potential research directions in this field, aiming to provide valuable insights for the construction of homologous expression systems with high-yield lipases.
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spelling doaj-art-adfbf69702504f25b46e1ec7cf9228842025-08-20T01:55:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15920591592059Expression regulation of bacterial lipase genes: a reviewDai-ming Zha0Yun-jun Yan1School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, ChinaKey Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaMicrobial lipases constitute the primary source of commercialized and industrial lipases, and they are extensively utilized across numerous industrial sectors. Compared to fungal lipases, bacterial lipases catalyze a broader spectrum of reactions with higher activity, enhanced stability, and improved stress resistance. Among them, lipases from Pseudomonas and Burkholderia cepacia are among the most widely employed microbial lipases. Furthermore, bacterial extracellular lipases act as crucial virulence factors, playing a significant role in the pathogenesis of bacteria. However, the production of bacterial lipases is typically low, rendering them expensive in the market and insufficient to meet the substantial demand for industrial production. To achieve large-scale production of bacterial lipases, stable and efficient homologous expression has proven to be an effective strategy. However, elucidating how bacterial lipase genes are regulated is the initial step for developing stable and efficient homologous expression, and a pressing scientific challenge. To date, the regulatory mechanisms governing the expression of bacterial lipase genes remain unclear, significantly impeding the construction of robust and high-yield homologous expression systems. Concurrently, understanding these regulatory mechanisms can facilitate early diagnosis of lipase-related pathogenic bacterial infections, and aid in the development of novel antibacterial drugs. In this review, we summarized the advancements in understanding the expression regulation of bacterial lipase genes, including direct regulators, the quorum sensing (QS) system, the Gac/Rsm system and its related regulators, as well as other regulators. Additionally, based on our ongoing research, we also discussed potential research directions in this field, aiming to provide valuable insights for the construction of homologous expression systems with high-yield lipases.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1592059/fulllipaseexpression regulationGac/Rsm systemQS systemtwo-component system
spellingShingle Dai-ming Zha
Yun-jun Yan
Expression regulation of bacterial lipase genes: a review
Frontiers in Microbiology
lipase
expression regulation
Gac/Rsm system
QS system
two-component system
title Expression regulation of bacterial lipase genes: a review
title_full Expression regulation of bacterial lipase genes: a review
title_fullStr Expression regulation of bacterial lipase genes: a review
title_full_unstemmed Expression regulation of bacterial lipase genes: a review
title_short Expression regulation of bacterial lipase genes: a review
title_sort expression regulation of bacterial lipase genes a review
topic lipase
expression regulation
Gac/Rsm system
QS system
two-component system
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1592059/full
work_keys_str_mv AT daimingzha expressionregulationofbacteriallipasegenesareview
AT yunjunyan expressionregulationofbacteriallipasegenesareview