Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing
The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Electrochemistry Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248125001390 |
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| author | Teodora Lupoi Bogdan Feier Florence Geneste Cecilia Cristea Yann R. Leroux |
| author_facet | Teodora Lupoi Bogdan Feier Florence Geneste Cecilia Cristea Yann R. Leroux |
| author_sort | Teodora Lupoi |
| collection | DOAJ |
| description | The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with ethynyl groups protected by trimethylsilyl (TMS) or triisopropylsilyl (TIPS) moieties. After deprotection, an azide-modified aptamer (APT) specific to diclofenac (DCF) was immobilized via copper-catalyzed azide–alkyne cycloaddition (CuAAC). Although the TMS and TIPS groups differ in size by only 1.7 Å, this small variation significantly influenced APT spacing and sensor performance. The TIPS-based sensor displayed a nearly fourfold increase in signal response compared to the TMS-based counterpart, achieving a limit of detection of 17.95 μM. These results underscore the importance of nanoscale molecular design in optimizing label-free aptasensor sensitivity. |
| format | Article |
| id | doaj-art-adfaec58249b4d99bc2d6d663f3d2d81 |
| institution | Kabale University |
| issn | 1388-2481 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj-art-adfaec58249b4d99bc2d6d663f3d2d812025-08-20T03:46:45ZengElsevierElectrochemistry Communications1388-24812025-09-0117810800010.1016/j.elecom.2025.108000Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensingTeodora Lupoi0Bogdan Feier1Florence Geneste2Cecilia Cristea3Yann R. Leroux4Univ Rennes, CNRS, ISCR, UMR 6226, F-35000 Rennes, France; “Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Analytical Chemistry, 400349 Cluj-Napoca, Romania“Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Analytical Chemistry, 400349 Cluj-Napoca, RomaniaUniv Rennes, CNRS, ISCR, UMR 6226, F-35000 Rennes, France“Iuliu Hatieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Analytical Chemistry, 400349 Cluj-Napoca, RomaniaUniv Rennes, CNRS, ISCR, UMR 6226, F-35000 Rennes, France; Corresponding author.The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with ethynyl groups protected by trimethylsilyl (TMS) or triisopropylsilyl (TIPS) moieties. After deprotection, an azide-modified aptamer (APT) specific to diclofenac (DCF) was immobilized via copper-catalyzed azide–alkyne cycloaddition (CuAAC). Although the TMS and TIPS groups differ in size by only 1.7 Å, this small variation significantly influenced APT spacing and sensor performance. The TIPS-based sensor displayed a nearly fourfold increase in signal response compared to the TMS-based counterpart, achieving a limit of detection of 17.95 μM. These results underscore the importance of nanoscale molecular design in optimizing label-free aptasensor sensitivity.http://www.sciencedirect.com/science/article/pii/S1388248125001390Controlled immobilizationClick-chemistryDiazonium graftingElectrochemical sensor |
| spellingShingle | Teodora Lupoi Bogdan Feier Florence Geneste Cecilia Cristea Yann R. Leroux Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing Electrochemistry Communications Controlled immobilization Click-chemistry Diazonium grafting Electrochemical sensor |
| title | Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing |
| title_full | Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing |
| title_fullStr | Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing |
| title_full_unstemmed | Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing |
| title_short | Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing |
| title_sort | surface organization of aptamers via diazonium grafting a key parameter in label free electrochemical sensing |
| topic | Controlled immobilization Click-chemistry Diazonium grafting Electrochemical sensor |
| url | http://www.sciencedirect.com/science/article/pii/S1388248125001390 |
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