Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics.
Evidence of the biological impacts of weak magnetic fields have been reported for more than fifty years. However, research progress on such effects has been hampered by a lack of systematics in most experiments. Efforts to increase the systematics in such cell biology experiments must include the ca...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0321133 |
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| author | Abasalt Bahrami Leonardo Y Tanaka Ricardo C Massucatto Francisco R M Laurindo Clarice D Aiello |
| author_facet | Abasalt Bahrami Leonardo Y Tanaka Ricardo C Massucatto Francisco R M Laurindo Clarice D Aiello |
| author_sort | Abasalt Bahrami |
| collection | DOAJ |
| description | Evidence of the biological impacts of weak magnetic fields have been reported for more than fifty years. However, research progress on such effects has been hampered by a lack of systematics in most experiments. Efforts to increase the systematics in such cell biology experiments must include the capability of producing fields that can be automatically adjusted and that are stable throughout an experiment's duration, usually operating inside an incubator. Here, we report on the design of a fully automated 1D Helmholtz coil setup that is internally water cooled, thus eliminating any confounding effects caused by temperature fluctuations. The coils also allow cells to be exposed to magnetic fields from multiple directions through automated controlled rotation. Preliminary data, acquired with the coils placed inside an incubator and on a rat vascular smooth muscle cell line, confirm previous reports that both microtubule and actin polymerization and dynamics are altered by weak magnetic fields. |
| format | Article |
| id | doaj-art-adf99968b2ae4e708c51cbfa2d52d3b1 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-adf99968b2ae4e708c51cbfa2d52d3b12025-08-20T02:55:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01208e032113310.1371/journal.pone.0321133Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics.Abasalt BahramiLeonardo Y TanakaRicardo C MassucattoFrancisco R M LaurindoClarice D AielloEvidence of the biological impacts of weak magnetic fields have been reported for more than fifty years. However, research progress on such effects has been hampered by a lack of systematics in most experiments. Efforts to increase the systematics in such cell biology experiments must include the capability of producing fields that can be automatically adjusted and that are stable throughout an experiment's duration, usually operating inside an incubator. Here, we report on the design of a fully automated 1D Helmholtz coil setup that is internally water cooled, thus eliminating any confounding effects caused by temperature fluctuations. The coils also allow cells to be exposed to magnetic fields from multiple directions through automated controlled rotation. Preliminary data, acquired with the coils placed inside an incubator and on a rat vascular smooth muscle cell line, confirm previous reports that both microtubule and actin polymerization and dynamics are altered by weak magnetic fields.https://doi.org/10.1371/journal.pone.0321133 |
| spellingShingle | Abasalt Bahrami Leonardo Y Tanaka Ricardo C Massucatto Francisco R M Laurindo Clarice D Aiello Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. PLoS ONE |
| title | Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. |
| title_full | Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. |
| title_fullStr | Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. |
| title_full_unstemmed | Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. |
| title_short | Automated 1D Helmholtz coil design for cell biology: Weak magnetic fields alter cytoskeleton dynamics. |
| title_sort | automated 1d helmholtz coil design for cell biology weak magnetic fields alter cytoskeleton dynamics |
| url | https://doi.org/10.1371/journal.pone.0321133 |
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