Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention
Abstract Bacterial biofilms on the surfaces of indwelling biomedical devices can cause long‐term infection and patient morbidity and mortality. Wrinkled surface topographies have previously demonstrated promising antifouling properties. Here we report a bioinspired strategy in which the actuation of...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-03-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400684 |
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| author | Elizabeth Oguntade Luiza Owuor Changling Du Anthony Acierto Sadie Meyer Mary Beth Browning Monroe James H. Henderson |
| author_facet | Elizabeth Oguntade Luiza Owuor Changling Du Anthony Acierto Sadie Meyer Mary Beth Browning Monroe James H. Henderson |
| author_sort | Elizabeth Oguntade |
| collection | DOAJ |
| description | Abstract Bacterial biofilms on the surfaces of indwelling biomedical devices can cause long‐term infection and patient morbidity and mortality. Wrinkled surface topographies have previously demonstrated promising antifouling properties. Here we report a bioinspired strategy in which the actuation of silk fibroin produces tunable, wrinkled surface topographies on 2D shape memory polymer (SMP) substrates and investigate the influence of these topographies on biofilm formation. To mimic biofilm‐associated infections related to the geometries of indwelling medical devices, silk wrinkles are produced on complex, 3D SMP architectures, and biofilm formation is evaluated. Using common biofilm‐causing agents, smaller silk wrinkle wavelengths and amplitudes are found to significantly reduce biofilm formation, resulting in primarily isolated, single‐cell bacteria on the 2D wrinkled surfaces. These single‐cell bacteria are nearly completely eradicated by treatment with antibiotics, which are ineffective against control surfaces. Antibiotics are also physically incorporated into the 2D wrinkled surfaces, which resulted in a further significant reduction in bacterial adhesion. Lastly, silk wrinkled topographies are successfully applied on 3D architectures, and the wrinkled surfaces display a significant reduction in biofilm coverage compared to controls. The findings demonstrate the potential for biopolymer wrinkles on biomaterials to be used as antifouling surfaces for biofilm prevention. |
| format | Article |
| id | doaj-art-adf4a0a6dd904928a7e5acb9fc137bdc |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-adf4a0a6dd904928a7e5acb9fc137bdc2025-08-20T01:49:11ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-03-01126n/an/a10.1002/admi.202400684Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm PreventionElizabeth Oguntade0Luiza Owuor1Changling Du2Anthony Acierto3Sadie Meyer4Mary Beth Browning Monroe5James H. Henderson6Department of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USADepartment of Biomedical & Chemical Engineering Syracuse University Syracuse NY 13244 USAAbstract Bacterial biofilms on the surfaces of indwelling biomedical devices can cause long‐term infection and patient morbidity and mortality. Wrinkled surface topographies have previously demonstrated promising antifouling properties. Here we report a bioinspired strategy in which the actuation of silk fibroin produces tunable, wrinkled surface topographies on 2D shape memory polymer (SMP) substrates and investigate the influence of these topographies on biofilm formation. To mimic biofilm‐associated infections related to the geometries of indwelling medical devices, silk wrinkles are produced on complex, 3D SMP architectures, and biofilm formation is evaluated. Using common biofilm‐causing agents, smaller silk wrinkle wavelengths and amplitudes are found to significantly reduce biofilm formation, resulting in primarily isolated, single‐cell bacteria on the 2D wrinkled surfaces. These single‐cell bacteria are nearly completely eradicated by treatment with antibiotics, which are ineffective against control surfaces. Antibiotics are also physically incorporated into the 2D wrinkled surfaces, which resulted in a further significant reduction in bacterial adhesion. Lastly, silk wrinkled topographies are successfully applied on 3D architectures, and the wrinkled surfaces display a significant reduction in biofilm coverage compared to controls. The findings demonstrate the potential for biopolymer wrinkles on biomaterials to be used as antifouling surfaces for biofilm prevention.https://doi.org/10.1002/admi.202400684antifouling surfacesbiofilm inhibitionshape memory polymersilk fibroinsurface topographies |
| spellingShingle | Elizabeth Oguntade Luiza Owuor Changling Du Anthony Acierto Sadie Meyer Mary Beth Browning Monroe James H. Henderson Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention Advanced Materials Interfaces antifouling surfaces biofilm inhibition shape memory polymer silk fibroin surface topographies |
| title | Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention |
| title_full | Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention |
| title_fullStr | Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention |
| title_full_unstemmed | Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention |
| title_short | Bacterial Response to Shape‐Memory Actuated Silk Wrinkled Surface Topographies as a Strategy for Biofilm Prevention |
| title_sort | bacterial response to shape memory actuated silk wrinkled surface topographies as a strategy for biofilm prevention |
| topic | antifouling surfaces biofilm inhibition shape memory polymer silk fibroin surface topographies |
| url | https://doi.org/10.1002/admi.202400684 |
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