Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling
The suppressor of cytokine signaling (SOCS) family of proteins were named after their defining role as negative feedback regulators of signaling initiated by numerous cytokine receptors. However, multiple members of the SOCS family likely function outside of this paradigm, including SOCS4. Zebrafish...
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MDPI AG
2024-08-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/9/1063 |
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| author | Monique Trengove Parisa Rasighaemi Clifford Liongue Alister C. Ward |
| author_facet | Monique Trengove Parisa Rasighaemi Clifford Liongue Alister C. Ward |
| author_sort | Monique Trengove |
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| description | The suppressor of cytokine signaling (SOCS) family of proteins were named after their defining role as negative feedback regulators of signaling initiated by numerous cytokine receptors. However, multiple members of the SOCS family likely function outside of this paradigm, including SOCS4. Zebrafish possess two <i>SOCS4</i> paralogues, with <i>socs4a</i> previously shown to participate in central nervous system development and function. This study examined the role of the other paralogue, <i>socs4b</i>, through expression analysis and functional investigations in vivo and in vitro. This revealed maternal deposition of <i>socs4b</i> mRNA, specific zygotic expression during late embryogenesis, including in the brain, eye and intestine, and broad adult expression that was highest in the brain. A mutant allele, <i>socs4bΔ18</i>, was generated by genome editing, in which the start codon was deleted. Fish homozygous for this likely hypomorphic allele showed no overt developmental phenotypes. However, in vitro studies suggested the Socs4b protein may be able to regulate EGFR signaling. |
| format | Article |
| id | doaj-art-adf0f106a46e42e8bf25c9e1ccde1391 |
| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-adf0f106a46e42e8bf25c9e1ccde13912025-08-20T01:56:00ZengMDPI AGBiomolecules2218-273X2024-08-01149106310.3390/biom14091063Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor SignalingMonique Trengove0Parisa Rasighaemi1Clifford Liongue2Alister C. Ward3School of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaThe suppressor of cytokine signaling (SOCS) family of proteins were named after their defining role as negative feedback regulators of signaling initiated by numerous cytokine receptors. However, multiple members of the SOCS family likely function outside of this paradigm, including SOCS4. Zebrafish possess two <i>SOCS4</i> paralogues, with <i>socs4a</i> previously shown to participate in central nervous system development and function. This study examined the role of the other paralogue, <i>socs4b</i>, through expression analysis and functional investigations in vivo and in vitro. This revealed maternal deposition of <i>socs4b</i> mRNA, specific zygotic expression during late embryogenesis, including in the brain, eye and intestine, and broad adult expression that was highest in the brain. A mutant allele, <i>socs4bΔ18</i>, was generated by genome editing, in which the start codon was deleted. Fish homozygous for this likely hypomorphic allele showed no overt developmental phenotypes. However, in vitro studies suggested the Socs4b protein may be able to regulate EGFR signaling.https://www.mdpi.com/2218-273X/14/9/1063cytokineEGFRSOCSSOCS4 |
| spellingShingle | Monique Trengove Parisa Rasighaemi Clifford Liongue Alister C. Ward Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling Biomolecules cytokine EGFR SOCS SOCS4 |
| title | Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling |
| title_full | Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling |
| title_fullStr | Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling |
| title_full_unstemmed | Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling |
| title_short | Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling |
| title_sort | zebrafish suppressor of cytokine signaling 4b socs4b is dispensable for development but may regulate epidermal growth factor receptor signaling |
| topic | cytokine EGFR SOCS SOCS4 |
| url | https://www.mdpi.com/2218-273X/14/9/1063 |
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