Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis

Abstract Acute kidney injury (AKI) is a significant global health issue, which is often caused by cisplatin therapy and characterized by mitochondrial dysfunction. Restoring mitochondrial homeostasis in tubular cells could exert therapeutic effects. Here, we investigated Slc25a21, a mitochondrial ca...

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Main Authors: Xin Su, Mi Bai, Yaqiong Shang, Yang Du, Shuang Xu, Xiuli Lin, Yunzhi Xiao, Yue Zhang, Huimei Chen, Aihua Zhang
Format: Article
Language:English
Published: Nature Publishing Group 2024-12-01
Series:Cell Death and Disease
Online Access:https://doi.org/10.1038/s41419-024-07231-2
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author Xin Su
Mi Bai
Yaqiong Shang
Yang Du
Shuang Xu
Xiuli Lin
Yunzhi Xiao
Yue Zhang
Huimei Chen
Aihua Zhang
author_facet Xin Su
Mi Bai
Yaqiong Shang
Yang Du
Shuang Xu
Xiuli Lin
Yunzhi Xiao
Yue Zhang
Huimei Chen
Aihua Zhang
author_sort Xin Su
collection DOAJ
description Abstract Acute kidney injury (AKI) is a significant global health issue, which is often caused by cisplatin therapy and characterized by mitochondrial dysfunction. Restoring mitochondrial homeostasis in tubular cells could exert therapeutic effects. Here, we investigated Slc25a21, a mitochondrial carrier, as a potential target for AKI intervention. Renal Slc25a21 expression is negatively associated with kidney function in both AKI patients and cisplatin-induced murine models. Sustaining renal expression of Slc25a21 slowed down AKI progression by reducing cellular apoptosis, necroptosis, and the inflammatory response, likely through its regulation of 2-oxoadipate conversion. Slc25a21 is highly expressed in proximal tubular epithelial cells, and its down-regulation contributes to compromised mitochondrial biogenesis and integrity, as well as impaired oxidative phosphorylation. Mechanistically, reduced Slc25a21 in AKI disrupts mitochondrial 2-oxoadipate transport, affecting related metabolites influx and the tricarboxylic acid cycle. These findings demonstrate a previously unappreciated metabolic function of Slc25a21 in tubular cells, and suggest that targeting mitochondrial metabolic homeostasis by sustaining Slc25a21 expression could be a potential novel therapeutic strategy for AKI.
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publisher Nature Publishing Group
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series Cell Death and Disease
spelling doaj-art-adeb9d11c4614b16bedc4e2a39ef56fb2025-08-20T01:57:13ZengNature Publishing GroupCell Death and Disease2041-48892024-12-01151211610.1038/s41419-024-07231-2Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasisXin Su0Mi Bai1Yaqiong Shang2Yang Du3Shuang Xu4Xiuli Lin5Yunzhi Xiao6Yue Zhang7Huimei Chen8Aihua Zhang9Department of Nephrology, Children’s Hospital of Nanjing Medical UniversityDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityCentre for Computational Biology and Programme in Cardiovascular and Metabolic Disorders, Duke-NUS Medical SchoolDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityCentre for Computational Biology and Programme in Cardiovascular and Metabolic Disorders, Duke-NUS Medical SchoolDepartment of Nephrology, Children’s Hospital of Nanjing Medical UniversityAbstract Acute kidney injury (AKI) is a significant global health issue, which is often caused by cisplatin therapy and characterized by mitochondrial dysfunction. Restoring mitochondrial homeostasis in tubular cells could exert therapeutic effects. Here, we investigated Slc25a21, a mitochondrial carrier, as a potential target for AKI intervention. Renal Slc25a21 expression is negatively associated with kidney function in both AKI patients and cisplatin-induced murine models. Sustaining renal expression of Slc25a21 slowed down AKI progression by reducing cellular apoptosis, necroptosis, and the inflammatory response, likely through its regulation of 2-oxoadipate conversion. Slc25a21 is highly expressed in proximal tubular epithelial cells, and its down-regulation contributes to compromised mitochondrial biogenesis and integrity, as well as impaired oxidative phosphorylation. Mechanistically, reduced Slc25a21 in AKI disrupts mitochondrial 2-oxoadipate transport, affecting related metabolites influx and the tricarboxylic acid cycle. These findings demonstrate a previously unappreciated metabolic function of Slc25a21 in tubular cells, and suggest that targeting mitochondrial metabolic homeostasis by sustaining Slc25a21 expression could be a potential novel therapeutic strategy for AKI.https://doi.org/10.1038/s41419-024-07231-2
spellingShingle Xin Su
Mi Bai
Yaqiong Shang
Yang Du
Shuang Xu
Xiuli Lin
Yunzhi Xiao
Yue Zhang
Huimei Chen
Aihua Zhang
Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
Cell Death and Disease
title Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
title_full Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
title_fullStr Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
title_full_unstemmed Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
title_short Slc25a21 in cisplatin-induced acute kidney injury: a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
title_sort slc25a21 in cisplatin induced acute kidney injury a new target for renal tubular epithelial protection by regulating mitochondrial metabolic homeostasis
url https://doi.org/10.1038/s41419-024-07231-2
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