Mechanism of virulence polymorphism in CR-hvKP strains from the same source
ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a major public health issue worldwide due to its high case fatality rate and transmissible nature. The plasmid containing drug-resistant and virulence genes obtained by CRKP can evolve into carbapenem-resistant hypervirulent Klebs...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2025-07-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02464-24 |
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| author | Jianhua Fang Hongyi Lai Miao Deng Yanfang Mei Dehua Chen Tieying Hou Tianxin Xiang |
| author_facet | Jianhua Fang Hongyi Lai Miao Deng Yanfang Mei Dehua Chen Tieying Hou Tianxin Xiang |
| author_sort | Jianhua Fang |
| collection | DOAJ |
| description | ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a major public health issue worldwide due to its high case fatality rate and transmissible nature. The plasmid containing drug-resistant and virulence genes obtained by CRKP can evolve into carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP), thus forming a new generation of superbacteria. However, systematic documentation of the mechanism of plasmid evolution that carries both drug resistance and virulence genes remains lacking. Fifty-four strains of CR-hvKP were collected here. The clinical data and bioinformatics were analyzed to explore the genomics and virulence plasmid evolution mechanism from the same source in the strains PFGE1-ST11-K64, PFGE2-ST11-K64, PFGE-ST11-K2, and PFGE-ST11-K47. The four groups from the same source exhibited virulent polymorphism, and mutations in the VirB11 gene may account for virulence variations across the strains of the same source.IMPORTANCEThis study emphasizes that in the process of clinical anti-infective treatment, attention should be paid not only to the strain itself but also to the external environment of the strain, especially the targeted therapeutic dose and course of host antibacterial drugs, which provides the possibility of diversity for the evolution of bacterial virulence and is conducive to the spread and survival of the CR-hvKP strain. |
| format | Article |
| id | doaj-art-adea623a7c294b3398a0a62425ecb83c |
| institution | OA Journals |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-adea623a7c294b3398a0a62425ecb83c2025-08-20T02:38:10ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-07-0113710.1128/spectrum.02464-24Mechanism of virulence polymorphism in CR-hvKP strains from the same sourceJianhua Fang0Hongyi Lai1Miao Deng2Yanfang Mei3Dehua Chen4Tieying Hou5Tianxin Xiang6Jiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaThe First Clinical Medical College, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaInfectious Diseases Department,The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaLaboratory Department,The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaMedical Experimental Center, Shenzhen Nanshan People's Hospital, The Sixth Affiliated Hospital of Shenzhen University Medical School, Shenzhen, Guangdong, ChinaJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, ChinaABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a major public health issue worldwide due to its high case fatality rate and transmissible nature. The plasmid containing drug-resistant and virulence genes obtained by CRKP can evolve into carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP), thus forming a new generation of superbacteria. However, systematic documentation of the mechanism of plasmid evolution that carries both drug resistance and virulence genes remains lacking. Fifty-four strains of CR-hvKP were collected here. The clinical data and bioinformatics were analyzed to explore the genomics and virulence plasmid evolution mechanism from the same source in the strains PFGE1-ST11-K64, PFGE2-ST11-K64, PFGE-ST11-K2, and PFGE-ST11-K47. The four groups from the same source exhibited virulent polymorphism, and mutations in the VirB11 gene may account for virulence variations across the strains of the same source.IMPORTANCEThis study emphasizes that in the process of clinical anti-infective treatment, attention should be paid not only to the strain itself but also to the external environment of the strain, especially the targeted therapeutic dose and course of host antibacterial drugs, which provides the possibility of diversity for the evolution of bacterial virulence and is conducive to the spread and survival of the CR-hvKP strain.https://journals.asm.org/doi/10.1128/spectrum.02464-24virulent polymorphismCR-hvKPsame source |
| spellingShingle | Jianhua Fang Hongyi Lai Miao Deng Yanfang Mei Dehua Chen Tieying Hou Tianxin Xiang Mechanism of virulence polymorphism in CR-hvKP strains from the same source Microbiology Spectrum virulent polymorphism CR-hvKP same source |
| title | Mechanism of virulence polymorphism in CR-hvKP strains from the same source |
| title_full | Mechanism of virulence polymorphism in CR-hvKP strains from the same source |
| title_fullStr | Mechanism of virulence polymorphism in CR-hvKP strains from the same source |
| title_full_unstemmed | Mechanism of virulence polymorphism in CR-hvKP strains from the same source |
| title_short | Mechanism of virulence polymorphism in CR-hvKP strains from the same source |
| title_sort | mechanism of virulence polymorphism in cr hvkp strains from the same source |
| topic | virulent polymorphism CR-hvKP same source |
| url | https://journals.asm.org/doi/10.1128/spectrum.02464-24 |
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