Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods
Aldehydes and ketones are volatile organic compounds (VOC) emitted into the atmosphere by a large number of natural and anthropogenic sources. Carbonyl compounds (CC) are atmospheric pollutants with known damaging effects for the human’s health. In this work, the separation of 31 carbonyl compounds...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2015/890836 |
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| author | Soraya de M. Ochs Maíra Fasciotti Annibal D. Pereira Netto |
| author_facet | Soraya de M. Ochs Maíra Fasciotti Annibal D. Pereira Netto |
| author_sort | Soraya de M. Ochs |
| collection | DOAJ |
| description | Aldehydes and ketones are volatile organic compounds (VOC) emitted into the atmosphere by a large number of natural and anthropogenic sources. Carbonyl compounds (CC) are atmospheric pollutants with known damaging effects for the human’s health. In this work, the separation of 31 carbonyl compounds (CC) in their 2,4-dinitrophenylhydrazones form was optimized by rapid resolution liquid chromatography in 9 minutes and simultaneously detected by ultraviolet and mass spectrometry with an APCI(−) as ionization source. The mass spectra of hydrazones presented the [M-H]− ions as base peak, but the MS/MS spectra showed fragments related to different structural classes of aldehydes and ketones, representing an important tool to assist structure elucidation of unknown CC in real samples. Multiple reactions monitoring (MRM) improved the sensitivity and selectivity for the quantitation method. Analytical parameters using both UV and MS (linearity, determination coefficients, detection limits, and sensitivity) were compared. The detection methods are complementary and a powerful analytical tool for the detection and quantitation of CC in complex environmental samples. |
| format | Article |
| id | doaj-art-ade7c96d45144eb9935971faf64df2cb |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-ade7c96d45144eb9935971faf64df2cb2025-02-03T07:25:05ZengWileyJournal of Spectroscopy2314-49202314-49392015-01-01201510.1155/2015/890836890836Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of MethodsSoraya de M. Ochs0Maíra Fasciotti1Annibal D. Pereira Netto2Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista, s/n, Centro, 24020-141 Niterói, RJ, BrazilInstituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO), Avenida Nossa Senhora das Graças 50, 25250-200 Duque de Caxias, RJ, BrazilPrograma de Pós-Graduação em Química, Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista, s/n, Centro, 24020-141 Niterói, RJ, BrazilAldehydes and ketones are volatile organic compounds (VOC) emitted into the atmosphere by a large number of natural and anthropogenic sources. Carbonyl compounds (CC) are atmospheric pollutants with known damaging effects for the human’s health. In this work, the separation of 31 carbonyl compounds (CC) in their 2,4-dinitrophenylhydrazones form was optimized by rapid resolution liquid chromatography in 9 minutes and simultaneously detected by ultraviolet and mass spectrometry with an APCI(−) as ionization source. The mass spectra of hydrazones presented the [M-H]− ions as base peak, but the MS/MS spectra showed fragments related to different structural classes of aldehydes and ketones, representing an important tool to assist structure elucidation of unknown CC in real samples. Multiple reactions monitoring (MRM) improved the sensitivity and selectivity for the quantitation method. Analytical parameters using both UV and MS (linearity, determination coefficients, detection limits, and sensitivity) were compared. The detection methods are complementary and a powerful analytical tool for the detection and quantitation of CC in complex environmental samples.http://dx.doi.org/10.1155/2015/890836 |
| spellingShingle | Soraya de M. Ochs Maíra Fasciotti Annibal D. Pereira Netto Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods Journal of Spectroscopy |
| title | Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods |
| title_full | Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods |
| title_fullStr | Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods |
| title_full_unstemmed | Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods |
| title_short | Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods |
| title_sort | analysis of 31 hydrazones of carbonyl compounds by rrlc uv and rrlc ms ms a comparison of methods |
| url | http://dx.doi.org/10.1155/2015/890836 |
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