DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation
Purpose: To evaluate DNA damage in lymphocytes and cognitive deficits in a nursing team occupationally exposed to ionizing radiation in a university hospital in southern Brazil. Method: Cross-sectional study, case-control design, included 79 women working in a nursing team in Hemodynamics, Radiology...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)
2015-06-01
|
| Series: | Brazilian Journal of Radiation Sciences |
| Online Access: | https://bjrs.org.br/revista/index.php/REVISTA/article/view/82 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849317900639272960 |
|---|---|
| author | Iranez Bortolotto Ana Paula S. Brum Larissa M. de Souza Cristiano Trindade Temenouga N. Guecheva Fabiano M Luiz Ana Ligia L de Paula-Ramos Angelica R. Consiglio |
| author_facet | Iranez Bortolotto Ana Paula S. Brum Larissa M. de Souza Cristiano Trindade Temenouga N. Guecheva Fabiano M Luiz Ana Ligia L de Paula-Ramos Angelica R. Consiglio |
| author_sort | Iranez Bortolotto |
| collection | DOAJ |
| description | Purpose: To evaluate DNA damage in lymphocytes and cognitive deficits in a nursing team occupationally exposed to ionizing radiation in a university hospital in southern Brazil. Method: Cross-sectional study, case-control design, included 79 women working in a nursing team in Hemodynamics, Radiology, Ambulatory and Midwifery Units. They were classified in two groups: 1_Exposed to ionizing radiation, n=38 (Hemodynamics and Radiology Units) and 2_Unexposed to ionizing radiation, n=41 (Ambulatory and Midwifery Unit). Blood was collected and DNA damage in lymphocytes was analyzed using the comet assay and micronucleus test (MN). The cells were classified according to the damage frequency (DF) and index (DI) based on the comet tail size. Cognition was also evaluated according to the scores obtained in Stroop test, the digit and word span. Results: No difference was found for the variables DI, DF, MN, Stroop_word, Stroop_color, Stroop_color/word between the groups. However, when the groups were analyzed independently of their exposure condition, the damage index in the Hemodynamics was lower than in all other Units. The damage frequency in Midwifery Unit was higher than in Ambulatory and Hemodynamics. The frequency of micronucleus was not different among the employees from the four Units. Conclusions: DNA damage in lymphocytes did not differ between nursing teams occupationally exposed or not exposed to ionizing radiation. However, higher damage in a non exposed group may indicate other risks to the healthcare team. |
| format | Article |
| id | doaj-art-ade6fdbdb93b43d1801e94213288aa0c |
| institution | Kabale University |
| issn | 2319-0612 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Brazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR) |
| record_format | Article |
| series | Brazilian Journal of Radiation Sciences |
| spelling | doaj-art-ade6fdbdb93b43d1801e94213288aa0c2025-08-20T03:51:03ZengBrazilian Radiation Protection Society (Sociedade Brasileira de Proteção Radiológica, SBPR)Brazilian Journal of Radiation Sciences2319-06122015-06-013110.15392/bjrs.v3i1.8264DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing RadiationIranez Bortolotto0Ana Paula S. Brum1Larissa M. de Souza2Cristiano Trindade3Temenouga N. Guecheva4Fabiano M Luiz5Ana Ligia L de Paula-Ramos6Angelica R. Consiglio7UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica e PPG_EnfermagemUFRGS_Universidade Federal do Rio Grande do Sul, PPG_EnfermagemPPG Biologia Molecular e CelularPPG Biologia Molecular e GenéticaUFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de BiofísicaUFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de BiofísicaUFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de BiofísicaUFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica e PPG_EnfermagemPurpose: To evaluate DNA damage in lymphocytes and cognitive deficits in a nursing team occupationally exposed to ionizing radiation in a university hospital in southern Brazil. Method: Cross-sectional study, case-control design, included 79 women working in a nursing team in Hemodynamics, Radiology, Ambulatory and Midwifery Units. They were classified in two groups: 1_Exposed to ionizing radiation, n=38 (Hemodynamics and Radiology Units) and 2_Unexposed to ionizing radiation, n=41 (Ambulatory and Midwifery Unit). Blood was collected and DNA damage in lymphocytes was analyzed using the comet assay and micronucleus test (MN). The cells were classified according to the damage frequency (DF) and index (DI) based on the comet tail size. Cognition was also evaluated according to the scores obtained in Stroop test, the digit and word span. Results: No difference was found for the variables DI, DF, MN, Stroop_word, Stroop_color, Stroop_color/word between the groups. However, when the groups were analyzed independently of their exposure condition, the damage index in the Hemodynamics was lower than in all other Units. The damage frequency in Midwifery Unit was higher than in Ambulatory and Hemodynamics. The frequency of micronucleus was not different among the employees from the four Units. Conclusions: DNA damage in lymphocytes did not differ between nursing teams occupationally exposed or not exposed to ionizing radiation. However, higher damage in a non exposed group may indicate other risks to the healthcare team.https://bjrs.org.br/revista/index.php/REVISTA/article/view/82 |
| spellingShingle | Iranez Bortolotto Ana Paula S. Brum Larissa M. de Souza Cristiano Trindade Temenouga N. Guecheva Fabiano M Luiz Ana Ligia L de Paula-Ramos Angelica R. Consiglio DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation Brazilian Journal of Radiation Sciences |
| title | DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation |
| title_full | DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation |
| title_fullStr | DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation |
| title_full_unstemmed | DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation |
| title_short | DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation |
| title_sort | dna damage evaluation in a nursing team occupationally exposed to ionizing radiation |
| url | https://bjrs.org.br/revista/index.php/REVISTA/article/view/82 |
| work_keys_str_mv | AT iranezbortolotto dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT anapaulasbrum dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT larissamdesouza dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT cristianotrindade dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT temenouganguecheva dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT fabianomluiz dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT analigialdepaularamos dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation AT angelicarconsiglio dnadamageevaluationinanursingteamoccupationallyexposedtoionizingradiation |