An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA)
Bacterial infections continue to pose a significant global health threat, further intensified by the increasing prevalence of antibiotic resistance. Methicillin-resistant Staphylococcus aureus (MRSA) poses significant threats, especially in healthcare settings around the world. Conventional treatmen...
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Journal of Pure and Applied Microbiology
2024-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/an-in-vitro-investigation-of-the-potential-synergistic-effect-of-3-hydrazinoquinoxaline-2-thiol-and-thymoquinones-against-methicillin-resistant-staphylococcus-aureus-mrsa/ |
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| author | Karem Ibrahem Wafaa Alhazmi Hanouf A. Niyazi Hatoon A. Niyazi Bandar Saleh Tariq Ekhmimi Jawahir A. Mokhtar Dalya Attallah Mohammed Bazuhair Khalil Alkuwaity Ahmad Sait Mohammed Mufrrih Mazen Ismail Yousef Almoghrabi Hussam Daghistani Ohood Alharbi Hisham Altayb Abdelbagi Alfadil |
| author_facet | Karem Ibrahem Wafaa Alhazmi Hanouf A. Niyazi Hatoon A. Niyazi Bandar Saleh Tariq Ekhmimi Jawahir A. Mokhtar Dalya Attallah Mohammed Bazuhair Khalil Alkuwaity Ahmad Sait Mohammed Mufrrih Mazen Ismail Yousef Almoghrabi Hussam Daghistani Ohood Alharbi Hisham Altayb Abdelbagi Alfadil |
| author_sort | Karem Ibrahem |
| collection | DOAJ |
| description | Bacterial infections continue to pose a significant global health threat, further intensified by the increasing prevalence of antibiotic resistance. Methicillin-resistant Staphylococcus aureus (MRSA) poses significant threats, especially in healthcare settings around the world. Conventional treatments face some limitations due to development of antimicrobial resistance, emphasizing the urgent need to explore alternative treatment strategies. In this study, we propose the potential synergistic activity of thymoquinone and 3-hydrazinoquinoxaline-2-thiol (3HT) to combat MRSA infections. The minimum inhibitory concentrations (MICs) of both thymoquinone and 3HT were assessed across twenty-two clinical MRSA strains. Thymoquinone demonstrated the minimum inhibitory concentration (MIC) values range between 8 and 128 µg/ml., while 3HT exhibited MIC levels varying from 16 to 32 µg/ml. Moreover, the checkerboard-assay was utilized to evaluate the integration of the following antimicrobial agents; thymoquinone and 3HT. Thymoquinone and 3HT bind to PBP2a leading to reduction in MRSA antimicrobial resistance via significantly disrupting its structure and function. Our study reveals an in-vitro synergistic interaction between these compounds with a fractional inhibitory concentration index (FICI) less than 0.5 against different isolated MRSA strains. While further research is necessary, our findings offer a promising approach for developing new effective MRSA treatments. |
| format | Article |
| id | doaj-art-adde8585b583400a891da0b16eef1752 |
| institution | DOAJ |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-adde8585b583400a891da0b16eef17522025-08-20T02:48:46ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-12-0118428372849https://doi.org/10.22207/JPAM.18.4.55An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA)Karem IbrahemWafaa Alhazmihttps://orcid.org/0000-0001-5322-0953Hanouf A. NiyaziHatoon A. NiyaziBandar SalehTariq EkhmimiJawahir A. MokhtarDalya AttallahMohammed BazuhairKhalil AlkuwaityAhmad SaitMohammed MufrrihMazen IsmailYousef AlmoghrabiHussam DaghistaniOhood AlharbiHisham Altayb Abdelbagi AlfadilBacterial infections continue to pose a significant global health threat, further intensified by the increasing prevalence of antibiotic resistance. Methicillin-resistant Staphylococcus aureus (MRSA) poses significant threats, especially in healthcare settings around the world. Conventional treatments face some limitations due to development of antimicrobial resistance, emphasizing the urgent need to explore alternative treatment strategies. In this study, we propose the potential synergistic activity of thymoquinone and 3-hydrazinoquinoxaline-2-thiol (3HT) to combat MRSA infections. The minimum inhibitory concentrations (MICs) of both thymoquinone and 3HT were assessed across twenty-two clinical MRSA strains. Thymoquinone demonstrated the minimum inhibitory concentration (MIC) values range between 8 and 128 µg/ml., while 3HT exhibited MIC levels varying from 16 to 32 µg/ml. Moreover, the checkerboard-assay was utilized to evaluate the integration of the following antimicrobial agents; thymoquinone and 3HT. Thymoquinone and 3HT bind to PBP2a leading to reduction in MRSA antimicrobial resistance via significantly disrupting its structure and function. Our study reveals an in-vitro synergistic interaction between these compounds with a fractional inhibitory concentration index (FICI) less than 0.5 against different isolated MRSA strains. While further research is necessary, our findings offer a promising approach for developing new effective MRSA treatments.https://microbiologyjournal.org/an-in-vitro-investigation-of-the-potential-synergistic-effect-of-3-hydrazinoquinoxaline-2-thiol-and-thymoquinones-against-methicillin-resistant-staphylococcus-aureus-mrsa/mrsaamr3htthymoquinoneantimicrobial agentsfici |
| spellingShingle | Karem Ibrahem Wafaa Alhazmi Hanouf A. Niyazi Hatoon A. Niyazi Bandar Saleh Tariq Ekhmimi Jawahir A. Mokhtar Dalya Attallah Mohammed Bazuhair Khalil Alkuwaity Ahmad Sait Mohammed Mufrrih Mazen Ismail Yousef Almoghrabi Hussam Daghistani Ohood Alharbi Hisham Altayb Abdelbagi Alfadil An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) Journal of Pure and Applied Microbiology mrsa amr 3ht thymoquinone antimicrobial agents fici |
| title | An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_full | An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_fullStr | An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_full_unstemmed | An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_short | An In vitro Investigation of the Potential Synergistic Effect of 3-Hydrazinoquinoxaline-2-Thiol and Thymoquinone’s against Methicillin Resistant Staphylococcus aureus (MRSA) |
| title_sort | in vitro investigation of the potential synergistic effect of 3 hydrazinoquinoxaline 2 thiol and thymoquinone s against methicillin resistant staphylococcus aureus mrsa |
| topic | mrsa amr 3ht thymoquinone antimicrobial agents fici |
| url | https://microbiologyjournal.org/an-in-vitro-investigation-of-the-potential-synergistic-effect-of-3-hydrazinoquinoxaline-2-thiol-and-thymoquinones-against-methicillin-resistant-staphylococcus-aureus-mrsa/ |
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