Development of an ELISPOT assay for numerating IFN-γ-secreting T cells in chicken using novel monoclonal antibodies
Cellular immune responses play critical roles in the control of pathogenic infection. The measurement of antigen-specific IFN-γ-secreting T cells via an enzyme-linked immunospot assay (ELISPOT) is a valuable method for the evaluation of cellular immune responses. However, in chicken, few of monoclon...
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| Main Authors: | Zhou Zhou, Ji Wu, Huining Zhang, Xinjie Yuan, Ye Meng, Lina Chen, Yi Yang, Xiaoli Hao, Shaobin Shang |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125007679 |
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