Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements
ABSTRACT: Objective: Infections due to carbapenemase-producing Enterobacterales harbouring more than one carbapenemase-encoding gene spread mainly by plasmid and transposon mobilisation. The objective of this study was to analyse the mobile genetic elements carrying blaKPC and blaNDM of Klebsiella...
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Elsevier
2025-05-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716525000542 |
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| author | Mayana Kieling Hernandez Berdichevski Rafaela R. Guerra Dariane C. Pereira Camila M. Wilhelm Patricia O. Barth Melise C. Silveira Fabiana C.Z. Volpato Claudio Rocha-de-Souza Richard M. Carrassai Ana Paula Carvalho-Assef Andreza F. Martins Afonso Luís Barth |
| author_facet | Mayana Kieling Hernandez Berdichevski Rafaela R. Guerra Dariane C. Pereira Camila M. Wilhelm Patricia O. Barth Melise C. Silveira Fabiana C.Z. Volpato Claudio Rocha-de-Souza Richard M. Carrassai Ana Paula Carvalho-Assef Andreza F. Martins Afonso Luís Barth |
| author_sort | Mayana Kieling Hernandez Berdichevski |
| collection | DOAJ |
| description | ABSTRACT: Objective: Infections due to carbapenemase-producing Enterobacterales harbouring more than one carbapenemase-encoding gene spread mainly by plasmid and transposon mobilisation. The objective of this study was to analyse the mobile genetic elements carrying blaKPC and blaNDM of Klebsiella pneumoniae carbapenemase co-producers (KpKN). Methods: K. pneumoniae isolates with reduced susceptibility to carbapenems were obtained between 2016 and 2023. To evaluate the genetic environment of KpKN, 22 isolates were selected for antimicrobial susceptibility testing and whole-genome sequencing. Results: The blaKPC-2 gene was carried mainly by IncN/IncFIB, a novel co-integrated plasmid in the Tn4401b transposon. blaNDM-1 was disseminated in the only two KpKN isolates recovered prior to 2020 by the IncHI1B/IncFIB plasmid type within the Tn3000 transposon. Significantly, isolates obtained since 2020 showed the blaNDM-1 gene carried by IncA/C in an IS26-flanked pseudo-composite transposon containing ISCR1, which also had genes that conferred resistance to sulfonamides, aminoglycosides, macrolides, quinolones, amphenicols, tetracyclines, rifampicin, sulfonamide, and trimethoprim. The isolates belonged mainly to ST11 and ST16. Conclusions: Plasmid and transposon changes during different periods could be related to higher dissemination of blaNDM-1, and the large number of resistance genes present in the IS26-flanked transposon may have increased co-selection of this plasmid through the wide use of antimicrobials during the pandemic. |
| format | Article |
| id | doaj-art-addc7f359fcb43b0866d240d83d505a7 |
| institution | DOAJ |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-addc7f359fcb43b0866d240d83d505a72025-08-20T02:57:18ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-05-014221422110.1016/j.jgar.2025.02.020Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elementsMayana Kieling Hernandez Berdichevski0Rafaela R. Guerra1Dariane C. Pereira2Camila M. Wilhelm3Patricia O. Barth4Melise C. Silveira5Fabiana C.Z. Volpato6Claudio Rocha-de-Souza7Richard M. Carrassai8Ana Paula Carvalho-Assef9Andreza F. Martins10Afonso Luís Barth11Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil; Corresponding author. Mailing address: Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Hospital de Clínicas de Porto Alegre (HCPA), Ramiro Barcelos 2350, Porto Alegre, RS, Brazil.Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil; Núcleo de Bioinformática, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilLaboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilLaboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilLaboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilInstituto Oswaldo Cruz, Fiocruz, Laboratório de Bacteriologia Aplicada à Saúde Única e Resistência Antimicrobiana, Rio de Janeiro, RJ, BrazilLaboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilInstituto Oswaldo Cruz, Fiocruz, Laboratório de Bacteriologia Aplicada à Saúde Única e Resistência Antimicrobiana, Rio de Janeiro, RJ, BrazilLaboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilInstituto Oswaldo Cruz, Fiocruz, Laboratório de Bacteriologia Aplicada à Saúde Única e Resistência Antimicrobiana, Rio de Janeiro, RJ, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil; Núcleo de Bioinformática, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, BrazilABSTRACT: Objective: Infections due to carbapenemase-producing Enterobacterales harbouring more than one carbapenemase-encoding gene spread mainly by plasmid and transposon mobilisation. The objective of this study was to analyse the mobile genetic elements carrying blaKPC and blaNDM of Klebsiella pneumoniae carbapenemase co-producers (KpKN). Methods: K. pneumoniae isolates with reduced susceptibility to carbapenems were obtained between 2016 and 2023. To evaluate the genetic environment of KpKN, 22 isolates were selected for antimicrobial susceptibility testing and whole-genome sequencing. Results: The blaKPC-2 gene was carried mainly by IncN/IncFIB, a novel co-integrated plasmid in the Tn4401b transposon. blaNDM-1 was disseminated in the only two KpKN isolates recovered prior to 2020 by the IncHI1B/IncFIB plasmid type within the Tn3000 transposon. Significantly, isolates obtained since 2020 showed the blaNDM-1 gene carried by IncA/C in an IS26-flanked pseudo-composite transposon containing ISCR1, which also had genes that conferred resistance to sulfonamides, aminoglycosides, macrolides, quinolones, amphenicols, tetracyclines, rifampicin, sulfonamide, and trimethoprim. The isolates belonged mainly to ST11 and ST16. Conclusions: Plasmid and transposon changes during different periods could be related to higher dissemination of blaNDM-1, and the large number of resistance genes present in the IS26-flanked transposon may have increased co-selection of this plasmid through the wide use of antimicrobials during the pandemic.http://www.sciencedirect.com/science/article/pii/S2213716525000542Co-integrated plasmidsBlaKPC-2BlaNDM-1K. pneumoniae co-harbouringBioinformaticsSpread of blaNDM-1 |
| spellingShingle | Mayana Kieling Hernandez Berdichevski Rafaela R. Guerra Dariane C. Pereira Camila M. Wilhelm Patricia O. Barth Melise C. Silveira Fabiana C.Z. Volpato Claudio Rocha-de-Souza Richard M. Carrassai Ana Paula Carvalho-Assef Andreza F. Martins Afonso Luís Barth Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements Journal of Global Antimicrobial Resistance Co-integrated plasmids BlaKPC-2 BlaNDM-1 K. pneumoniae co-harbouring Bioinformatics Spread of blaNDM-1 |
| title | Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements |
| title_full | Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements |
| title_fullStr | Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements |
| title_full_unstemmed | Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements |
| title_short | Plasmidome analyses of Klebsiella pneumoniae co-producing blaKPC-2 and blaNDM-1 in Southern Brazil: characterisation of mobile genetic elements |
| title_sort | plasmidome analyses of klebsiella pneumoniae co producing blakpc 2 and blandm 1 in southern brazil characterisation of mobile genetic elements |
| topic | Co-integrated plasmids BlaKPC-2 BlaNDM-1 K. pneumoniae co-harbouring Bioinformatics Spread of blaNDM-1 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716525000542 |
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