Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles
Inconsistent fluctuation of quinolizidine alkaloid (QA) content in lupin seeds is often observed, sometimes surpassing safe thresholds (>200 mg/kg) yet lacks a clear explanation. This study aimed to investigate the intra-plant variability of QA content in three narrow-leaf lupin genotypes: Tanjil...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025007479 |
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| author | Tawfeek Khedr Ling-Ling Gao Lars G. Kamphuis Angéla Juhász Michelle L. Colgrave |
| author_facet | Tawfeek Khedr Ling-Ling Gao Lars G. Kamphuis Angéla Juhász Michelle L. Colgrave |
| author_sort | Tawfeek Khedr |
| collection | DOAJ |
| description | Inconsistent fluctuation of quinolizidine alkaloid (QA) content in lupin seeds is often observed, sometimes surpassing safe thresholds (>200 mg/kg) yet lacks a clear explanation. This study aimed to investigate the intra-plant variability of QA content in three narrow-leaf lupin genotypes: Tanjil, Quillinock, and P22660 using LC-MS/MS. QA levels in seeds from the same pods showed <30 % variation, while those from different pods in the same plant sometimes surpassed inter-plant variations (up to 94 %). The average measured QA concentrations under speed breeding conditions in Quillinock, Tanjil, and P22660 were 36, 103, and 27,000 mg/kg, respectively. The impact of longer daylight hours (16h day/8h night), a practice used in speed breeding, on QA content in the cultivar Tanjil was also studied. Longer daylight hours yielded elevated QA levels in Tanjil seeds (103 mg/kg) compared to those grown in a greenhouse (10–13h daylight, 32 mg/kg). |
| format | Article |
| id | doaj-art-add68b9cccab47ba8aa7e5f4da5e6428 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-add68b9cccab47ba8aa7e5f4da5e64282025-02-05T04:32:19ZengElsevierHeliyon2405-84402025-02-01113e42367Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cyclesTawfeek Khedr0Ling-Ling Gao1Lars G. Kamphuis2Angéla Juhász3Michelle L. Colgrave4School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, 6027, AustraliaCSIRO Agriculture and Food, Underwood Avenue, Floreat, WA, 6014, AustraliaCSIRO Agriculture and Food, Underwood Avenue, Floreat, WA, 6014, Australia; Centre for Crop and Disease Management, School of Molecular and Life Science, Curtin University, Bentley, WA, 6102, AustraliaSchool of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, 6027, AustraliaSchool of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, 6027, Australia; CSIRO Agriculture and Food, Underwood Avenue, Floreat, WA, 6014, Australia; Corresponding author. School of Science, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, 6027, Australia.Inconsistent fluctuation of quinolizidine alkaloid (QA) content in lupin seeds is often observed, sometimes surpassing safe thresholds (>200 mg/kg) yet lacks a clear explanation. This study aimed to investigate the intra-plant variability of QA content in three narrow-leaf lupin genotypes: Tanjil, Quillinock, and P22660 using LC-MS/MS. QA levels in seeds from the same pods showed <30 % variation, while those from different pods in the same plant sometimes surpassed inter-plant variations (up to 94 %). The average measured QA concentrations under speed breeding conditions in Quillinock, Tanjil, and P22660 were 36, 103, and 27,000 mg/kg, respectively. The impact of longer daylight hours (16h day/8h night), a practice used in speed breeding, on QA content in the cultivar Tanjil was also studied. Longer daylight hours yielded elevated QA levels in Tanjil seeds (103 mg/kg) compared to those grown in a greenhouse (10–13h daylight, 32 mg/kg).http://www.sciencedirect.com/science/article/pii/S2405844025007479Secondary metabolitesLupinus speciesAbiotic stressSpeed breeding |
| spellingShingle | Tawfeek Khedr Ling-Ling Gao Lars G. Kamphuis Angéla Juhász Michelle L. Colgrave Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles Heliyon Secondary metabolites Lupinus species Abiotic stress Speed breeding |
| title | Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles |
| title_full | Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles |
| title_fullStr | Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles |
| title_full_unstemmed | Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles |
| title_short | Intra- and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles |
| title_sort | intra and inter plant variability of quinolizidine alkaloids in narrow leaf lupin seeds grown under different day night cycles |
| topic | Secondary metabolites Lupinus species Abiotic stress Speed breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025007479 |
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